(Linear) (Six-base) MAPSORT of: KIEE2266.seq Check: 4386 from: 1 to: 2556 KIEE2266.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 612 671 1205 1334 1948 2556 Size: 592 20 59 534 129 614 608 Fragments arranged by size: 614 608 592 534 129 59 20 AflIII A'CryG_T Cuts at: 0 276 874 1207 2281 2556 Size: 276 598 333 1074 275 Fragments arranged by size: 1074 598 333 276 275 AleI CACnn'nnGTG Cuts at: 0 58 1006 2263 2556 Size: 58 948 1257 293 Fragments arranged by size: 1257 948 293 58 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2325 2357 2556 Size: 2325 32 199 Fragments arranged by size: 2325 199 32 AlwNI CAG_nnn'CTG Cuts at: 0 1239 2133 2556 Size: 1239 894 423 Fragments arranged by size: 1239 894 423 ApoI r'AATT_y Cuts at: 0 701 823 2556 Size: 701 122 1733 Fragments arranged by size: 1733 701 122 AseI AT'TA_AT Cuts at: 0 1363 2556 Size: 1363 1193 AvaI C'yCGr_G Cuts at: 0 14 2556 Size: 14 2542 BanI G'GyrC_C Cuts at: 0 475 991 2556 Size: 475 516 1565 Fragments arranged by size: 1565 516 475 BanII G_rGCy'C Cuts at: 0 15 899 1136 1702 2556 Size: 15 884 237 566 854 Fragments arranged by size: 884 854 566 237 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 606 1672 2556 Size: 606 1066 884 Fragments arranged by size: 1066 884 606 BciVI GTATCCnnnnn_n' Cuts at: 0 1969 2556 Size: 1969 587 BclI T'GATC_A Cuts at: 0 2309 2556 Size: 2309 247 BglII A'GATC_T Cuts at: 0 91 2556 Size: 91 2465 BlpI GC'TnA_GC Cuts at: 0 1109 2134 2556 Size: 1109 1025 422 Fragments arranged by size: 1109 1025 422 BmgBI CAC'GTC Cuts at: 0 875 1210 1896 2556 Size: 875 335 686 660 Fragments arranged by size: 875 686 660 335 BmrI ACTGGGnnnn_n' Cuts at: 0 41 1705 2516 2556 Size: 41 1664 811 40 Fragments arranged by size: 1664 811 41 40 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2033 2556 Size: 2033 523 Bpu10I CC'TnA_GC Cuts at: 0 1130 2556 Size: 1130 1426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1536 2154 2556 Size: 1536 618 402 Fragments arranged by size: 1536 618 402 BsaI GGTCTCn'nnnn_ Cuts at: 0 1288 2556 Size: 1288 1268 BsaAI yAC'GTr Cuts at: 0 2284 2556 Size: 2284 272 BsaBI GATnn'nnATC Cuts at: 0 562 1768 2556 Size: 562 1206 788 Fragments arranged by size: 1206 788 562 BsaWI w'CCGG_w Cuts at: 0 803 2556 Size: 803 1753 BseYI C'CCAG_C Cuts at: 0 707 2457 2556 Size: 707 1750 99 Fragments arranged by size: 1750 707 99 BsiHKAI G_wGCw'C Cuts at: 0 1136 1702 2019 2556 Size: 1136 566 317 537 Fragments arranged by size: 1136 566 537 317 BsmI GAATG_Cn' Cuts at: 0 466 1646 2507 2556 Size: 466 1180 861 49 Fragments arranged by size: 1180 861 466 49 BsmBI CGTCTCn'nnnn_ Cuts at: 0 805 1216 2556 Size: 805 411 1340 Fragments arranged by size: 1340 805 411 Bsp1286I G_dGCh'C Cuts at: 0 15 899 1136 1702 2019 2556 Size: 15 884 237 566 317 537 Fragments arranged by size: 884 566 537 317 237 15 BspEI T'CCGG_A Cuts at: 0 803 2556 Size: 803 1753 BspHI T'CATG_A Cuts at: 0 827 2006 2043 2556 Size: 827 1179 37 513 Fragments arranged by size: 1179 827 513 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1519 2556 Size: 1519 1037 BsrDI GCAATG_nn' Cuts at: 0 82 135 2556 Size: 82 53 2421 Fragments arranged by size: 2421 82 53 BsrFI r'CCGG_y Cuts at: 0 1226 2556 Size: 1226 1330 BsrGI T'GTAC_A Cuts at: 0 2238 2556 Size: 2238 318 BstBI TT'CG_AA Cuts at: 0 1419 2556 Size: 1419 1137 BstXI CCAn_nnnn'nTGG Cuts at: 0 60 1251 2556 Size: 60 1191 1305 Fragments arranged by size: 1305 1191 60 BstYI r'GATC_y Cuts at: 0 91 152 635 2556 Size: 91 61 483 1921 Fragments arranged by size: 1921 483 91 61 BtgI C'CryG_G Cuts at: 0 1535 2556 Size: 1535 1021 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 18 2556 Size: 18 2538 BtsI GCAGTG_nn' Cuts at: 0 2145 2556 Size: 2145 411 DraI TTT'AAA Cuts at: 0 1436 1841 2556 Size: 1436 405 715 Fragments arranged by size: 1436 715 405 DraIII CAC_nnn'GTG Cuts at: 0 1943 2556 Size: 1943 613 DrdI GACnn_nn'nnGTC Cuts at: 0 1711 2556 Size: 1711 845 EaeI y'GGCC_r Cuts at: 0 1241 1335 2437 2556 Size: 1241 94 1102 119 Fragments arranged by size: 1241 1102 119 94 EarI CTCTTCn'nnn_ Cuts at: 0 3 568 1081 1515 2551 2556 Size: 3 565 513 434 1036 5 Fragments arranged by size: 1036 565 513 434 5 3 EcoICRI GAG'CTC Cuts at: 0 1134 1700 2556 Size: 1134 566 856 Fragments arranged by size: 1134 856 566 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 612 671 1205 1334 1948 2033 2556 Size: 592 20 59 534 129 614 85 523 Fragments arranged by size: 614 592 534 523 129 85 59 20 EcoRI G'AATT_C Cuts at: 0 823 2556 Size: 823 1733 EcoRV GAT'ATC Cuts at: 0 179 1325 2556 Size: 179 1146 1231 Fragments arranged by size: 1231 1146 179 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2505 2556 Size: 2505 51 HaeII r_GCGC'y Cuts at: 0 1239 2556 Size: 1239 1317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 388 420 571 603 2556 Size: 388 32 151 32 1953 Fragments arranged by size: 1953 388 151 32 32 HincII GTy'rAC Cuts at: 0 1384 1926 2556 Size: 1384 542 630 Fragments arranged by size: 1384 630 542 HpaI GTT'AAC Cuts at: 0 1926 2556 Size: 1926 630 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 476 1887 2556 Size: 476 1411 669 Fragments arranged by size: 1411 669 476 MscI TGG'CCA Cuts at: 0 1243 2556 Size: 1243 1313 MslI CAynn'nnrTG Cuts at: 0 58 745 1006 1762 2263 2556 Size: 58 687 261 756 501 293 Fragments arranged by size: 756 687 501 293 261 58 MspA1I CmG'CkG Cuts at: 0 1182 1588 1635 1955 2097 2130 2556 Size: 1182 406 47 320 142 33 426 Fragments arranged by size: 1182 426 406 320 142 47 33 NaeI GCC'GGC Cuts at: 0 1228 2556 Size: 1228 1328 NcoI C'CATG_G Cuts at: 0 1535 2556 Size: 1535 1021 NdeI CA'TA_TG Cuts at: 0 1378 2393 2556 Size: 1378 1015 163 Fragments arranged by size: 1378 1015 163 NgoMIV G'CCGG_C Cuts at: 0 1226 2556 Size: 1226 1330 NspI r_CATG'y Cuts at: 0 280 2208 2556 Size: 280 1928 348 Fragments arranged by size: 1928 348 280 PciI A'CATG_T Cuts at: 0 276 2556 Size: 276 2280 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 282 314 2556 Size: 282 32 2242 Fragments arranged by size: 2242 282 32 PvuII CAG'CTG Cuts at: 0 1182 1588 1635 2097 2130 2556 Size: 1182 406 47 462 33 426 Fragments arranged by size: 1182 462 426 406 47 33 SacI G_AGCT'C Cuts at: 0 1136 1702 2556 Size: 1136 566 854 Fragments arranged by size: 1136 854 566 SapI GCTCTTCn'nnn_ Cuts at: 0 3 2556 Size: 3 2553 SexAI A'CCwGG_T Cuts at: 0 1456 1939 2556 Size: 1456 483 617 Fragments arranged by size: 1456 617 483 SmlI C'TyrA_G Cuts at: 0 1515 2169 2556 Size: 1515 654 387 Fragments arranged by size: 1515 654 387 SphI G_CATG'C Cuts at: 0 2208 2556 Size: 2208 348 SspI AAT'ATT Cuts at: 0 1361 1693 2556 Size: 1361 332 863 Fragments arranged by size: 1361 863 332 StyI C'CwwG_G Cuts at: 0 1409 1535 2556 Size: 1409 126 1021 Fragments arranged by size: 1409 1021 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 883 2556 Size: 883 1673 TatI w'GTAC_w Cuts at: 0 1572 2036 2238 2426 2556 Size: 1572 464 202 188 130 Fragments arranged by size: 1572 464 202 188 130 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 299 331 703 735 1532 1564 2556 Size: 299 32 372 32 797 32 992 Fragments arranged by size: 992 797 372 299 32 32 32 XbaI T'CTAG_A Cuts at: 0 406 2556 Size: 406 2150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 267 2556 Size: 267 2289 XmnI GAAnn'nnTTC Cuts at: 0 2002 2556 Size: 2002 554 Enzymes that do cut: AcuI AflIII AleI AloI AlwNI ApoI AseI AvaI BanI BanII BbsI BciVI BclI BglII BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoRI EcoRV FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PciI PsrI PvuII SacI SapI SexAI SmlI SphI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI Bme1580I BmtI BplI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsrBI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI EagI EciI EcoNI EcoO109I FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI ZraI