(Linear) (Six-base) MAPSORT of: KIEE4515.seq Check: 7969 from: 1 to: 1641 KIEE4515.orf With 182 enzymes: SgfI * March 22, 2012 18:15 .. AccI GT'mk_AC Cuts at: 0 104 430 1641 Size: 104 326 1211 Fragments arranged by size: 1211 326 104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 668 692 721 1231 1641 Size: 26 642 24 29 510 410 Fragments arranged by size: 642 510 410 29 26 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1032 1641 Size: 1032 609 AlwNI CAG_nnn'CTG Cuts at: 0 699 1641 Size: 699 942 ApoI r'AATT_y Cuts at: 0 399 1060 1141 1641 Size: 399 661 81 500 Fragments arranged by size: 661 500 399 81 AvaI C'yCGr_G Cuts at: 0 835 1641 Size: 835 806 BamHI G'GATC_C Cuts at: 0 751 1641 Size: 751 890 BanI G'GyrC_C Cuts at: 0 117 1349 1622 1641 Size: 117 1232 273 19 Fragments arranged by size: 1232 273 117 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 837 1527 1641 Size: 837 690 114 Fragments arranged by size: 837 690 114 BbvCI CC'TCA_GC Cuts at: 0 1552 1641 Size: 1552 89 BfrBI ATG'CAT Cuts at: 0 1243 1641 Size: 1243 398 BglI GCCn_nnn'nGGC Cuts at: 0 703 1495 1641 Size: 703 792 146 Fragments arranged by size: 792 703 146 BlpI GC'TnA_GC Cuts at: 0 1093 1641 Size: 1093 548 Bme1580I G_kGCm'C Cuts at: 0 1352 1641 Size: 1352 289 BmrI ACTGGGnnnn_n' Cuts at: 0 750 1641 Size: 750 891 BmtI G_CTAG'C Cuts at: 0 817 1641 Size: 817 824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 579 728 1641 Size: 46 533 149 913 Fragments arranged by size: 913 533 149 46 Bpu10I CC'TnA_GC Cuts at: 0 1552 1641 Size: 1552 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 19 162 1641 Size: 19 143 1479 Fragments arranged by size: 1479 143 19 BsaAI yAC'GTr Cuts at: 0 189 1304 1641 Size: 189 1115 337 Fragments arranged by size: 1115 337 189 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1232 1641 Size: 1232 409 BsiHKAI G_wGCw'C Cuts at: 0 148 1099 1641 Size: 148 951 542 Fragments arranged by size: 951 542 148 BsmI GAATG_Cn' Cuts at: 0 379 1116 1243 1641 Size: 379 737 127 398 Fragments arranged by size: 737 398 379 127 Bsp1286I G_dGCh'C Cuts at: 0 148 1099 1352 1641 Size: 148 951 253 289 Fragments arranged by size: 951 289 253 148 BspHI T'CATG_A Cuts at: 0 597 1641 Size: 597 1044 BsrDI GCAATG_nn' Cuts at: 0 665 824 1641 Size: 665 159 817 Fragments arranged by size: 817 665 159 BsrGI T'GTAC_A Cuts at: 0 797 1375 1641 Size: 797 578 266 Fragments arranged by size: 797 578 266 BstAPI GCAn_nnn'nTGC Cuts at: 0 823 1641 Size: 823 818 BstXI CCAn_nnnn'nTGG Cuts at: 0 792 1641 Size: 792 849 BstYI r'GATC_y Cuts at: 0 751 1641 Size: 751 890 BstZ17I GTA'TAC Cuts at: 0 431 1641 Size: 431 1210 Bsu36I CC'TnA_GG Cuts at: 0 1070 1641 Size: 1070 571 BtgI C'CryG_G Cuts at: 0 14 1641 Size: 14 1627 BtsI GCAGTG_nn' Cuts at: 0 860 1641 Size: 860 781 DrdI GACnn_nn'nnGTC Cuts at: 0 787 1641 Size: 787 854 EaeI y'GGCC_r Cuts at: 0 11 1641 Size: 11 1630 EarI CTCTTCn'nnn_ Cuts at: 0 339 644 1207 1261 1641 Size: 339 305 563 54 380 Fragments arranged by size: 563 380 339 305 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 46 579 668 692 721 728 1231 Size: 26 20 533 89 24 29 7 503 Cuts at: 1231 1641 Size: 410 Fragments arranged by size: 533 503 410 89 29 26 24 20 7 EcoO109I rG'GnC_Cy Cuts at: 0 696 1641 Size: 696 945 EcoRI G'AATT_C Cuts at: 0 1141 1641 Size: 1141 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 1048 1063 1080 1095 1317 1349 Size: 316 32 700 15 17 15 222 32 Cuts at: 1349 1641 Size: 292 Fragments arranged by size: 700 316 292 222 32 32 17 15 15 HincII GTy'rAC Cuts at: 0 324 1641 Size: 324 1317 HindIII A'AGCT_T Cuts at: 0 993 1182 1544 1641 Size: 993 189 362 97 Fragments arranged by size: 993 362 189 97 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 362 1641 Size: 362 1279 MslI CAynn'nnrTG Cuts at: 0 1489 1641 Size: 1489 152 MspA1I CmG'CkG Cuts at: 0 1027 1641 Size: 1027 614 NdeI CA'TA_TG Cuts at: 0 951 1239 1641 Size: 951 288 402 Fragments arranged by size: 951 402 288 NheI G'CTAG_C Cuts at: 0 813 1641 Size: 813 828 NsiI A_TGCA'T Cuts at: 0 1245 1641 Size: 1245 396 NspI r_CATG'y Cuts at: 0 159 1641 Size: 159 1482 PflMI CCAn_nnn'nTGG Cuts at: 0 1619 1641 Size: 1619 22 PfoI T'CCnGG_A Cuts at: 0 1517 1641 Size: 1517 124 PstI C_TGCA'G Cuts at: 0 594 1641 Size: 594 1047 PvuII CAG'CTG Cuts at: 0 1027 1641 Size: 1027 614 SfcI C'TryA_G Cuts at: 0 590 1641 Size: 590 1051 SmlI C'TyrA_G Cuts at: 0 34 141 1641 Size: 34 107 1500 Fragments arranged by size: 1500 107 34 StyI C'CwwG_G Cuts at: 0 605 1337 1641 Size: 605 732 304 Fragments arranged by size: 732 605 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 426 1406 1641 Size: 426 980 235 Fragments arranged by size: 980 426 235 TatI w'GTAC_w Cuts at: 0 797 1375 1641 Size: 797 578 266 Fragments arranged by size: 797 578 266 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1499 1531 1641 Size: 1499 32 110 Fragments arranged by size: 1499 110 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 21 1616 1641 Size: 21 1595 25 Fragments arranged by size: 1595 25 21 XmnI GAAnn'nnTTC Cuts at: 0 883 932 1641 Size: 883 49 709 Fragments arranged by size: 883 709 49 Enzymes that do cut: AccI AcuI AlfI AlwNI ApoI AvaI BamHI BanI BbsI BbvCI BfrBI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaAI BseRI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI MspA1I NdeI NheI NsiI NspI PflMI PfoI PstI PvuII SfcI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BcgI BciVI BclI BglII BmgBI BplI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NotI NruI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI