Restriction map for
pFN21AE3640
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BcnI BfrI BlnI BlpI BmyI
Bpu1102I BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII HhaI HinP1I HindIII
KasI KpnI KspI MluI MspA1I
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI Sse8387I SspBI
StuI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 2145 TCCGGA T^CCGGA
AcyI 288 GACGCC G[GA]^CG[CT]C
AlwI 1831 GATCC GGATC(4/5)
BanI 1672 GGTGCC G^G[CT][GA]CC
BcgI 1943 CGAATAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 233 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 295 AGATCT A^GATCT
BsaAI 349 TACGTA [CT]AC^GT[GA]
BsaHI 288 GACGCC G[GA]^CG[CT]C
BsaI 805 GAGACC GGTCTC(1/5)
BsaWI 2145 TCCGGA [AT]^CCGG[AT]
BseAI 2145 TCCGGA T^CCGGA
BseRI 2063 CTCCTC GAGGAG(10/8)
BsgI 69 GTGCAG GTGCAG(16/14)
BsiYI 809 CCAAAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 809 CCAAAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1152 GTCCC GGGAC(10/14)
BsmI 1740 GAATGC GAATGC(1/-1)
BspDI 37 ATCGAT AT^CGAT
BspEI 2145 TCCGGA T^CCGGA
BspHI 1131 TCATGA T^CATGA
BsrDI 1694 GCAATG GCAATG(2/0)
BstAPI 2400 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 37 ATCGAT AT^CGAT
DraII 759 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 759 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 558 GAATTC G^AATTC
EcoT22I 1507 ATGCAT ATGCA^T
HapII 2146 CCGG C^CGG
HgaI 288 GACGC GACGC(5/10)
Hin1I 288 GACGCC G[GA]^CG[CT]C
HpaI 2125 GTTAAC GTT^AAC
HpaII 2146 CCGG C^CGG
MaeII 350 ACGT A^CGT
MfeI 692 CAATTG C^AATTG
MroI 2145 TCCGGA T^CCGGA
MspI 2146 CCGG C^CGG
MunI 692 CAATTG C^AATTG
NsiI 1507 ATGCAT ATGCA^T
PleI 587 GACTC GAGTC(4/5)
RcaI 1131 TCATGA T^CATGA
SapI 1747 GCTCTTC GCTCTTC(1/4)
ScaI 2421 AGTACT AGT^ACT
SmlI 748 CTTGAG C^T[CT][GA]AG
SnaBI 349 TACGTA TAC^GTA
SpeI 1087 ACTAGT A^CTAGT
SphI 267 GCATGC GCATG^C
XcmI 234 CCATCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 607 ACATGT A^C[GA][CT]GT
2394 ACATGT
AlwNI 898 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1835 CAGAAACTG
AvaII 366 GGTCC G^G[AT]CC
1987 GGACC
BalI 231 TGGCCA TGG^CCA
1545 TGGCCA
BbsI 1361 GAAGAC GAAGAC(2/6)
1426 GAAGAC
BfaI 1088 CTAG C^TAG
1658 CTAG
BpuAI 1361 GAAGAC GAAGAC(2/6)
1426 GAAGAC
BsaJI 1124 CCTTGG C^C[ACGT][ACGT]GG
2285 CCAAGG
BspLU11I 607 ACATGT A^CATGT
2394 ACATGT
BstYI 295 AGATCT [GA]^GATC[CT]
1830 AGATCC
EaeI 231 TGGCCA [CT]^GGCC[GA]
1545 TGGCCA
EarI 1748 CTCTTC CTCTTC(1/4)
2078 CTCTTC
EcoT14I 1124 CCTTGG C^C[AT][AT]GG
2285 CCAAGG
Ksp632I 1748 CTCTTC CTCTTC(1/4)
2078 CTCTTC
MaeI 1088 CTAG C^TAG
1658 CTAG
MflI 295 AGATCT [GA]^GATC[CT]
1830 AGATCC
MluNI 231 TGGCCA TGG^CCA
1545 TGGCCA
MscI 231 TGGCCA TGG^CCA
1545 TGGCCA
NlaIV 1672 GGTGCC GG[ACGT]^[ACGT]CC
2163 GGCTCC
StyI 1124 CCTTGG C^C[AT][AT]GG
2285 CCAAGG
SwaI 1394 ATTTAAAT ATTT^AAAT
1664 ATTTAAAT
XhoII 295 AGATCT [GA]^GATC[CT]
1830 AGATCC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BclI BpmI BsaBI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeIII
MamI MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I