(Circular) (Six-base) MAPSORT of: pFN21AE3640.seq Check: 8215 from: 1 to: 7171 pFN21AE3640 7171 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4514 1937 Size: 2577 4594 Acc65I G'GTAC_C Cuts at: 4492 4492 Size: 7171 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2208 3450 5234 6618 6886 1632 Size: 564 12 1242 1784 1384 268 1917 Fragments arranged by size: 1917 1784 1384 1242 564 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6974 AflIII A'CryG_T Cuts at: 1203 2612 4399 6070 7047 1203 Size: 1409 1787 1671 977 1327 Fragments arranged by size: 1787 1671 1409 1327 977 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 5900 5900 Size: 7171 AleI CACnn'nnGTG Cuts at: 1166 4400 1166 Size: 3234 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4296 1184 Size: 3112 4059 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2019 Size: 32 7139 AlwNI CAG_nnn'CTG Cuts at: 2908 3845 4914 6486 2908 Size: 937 1069 1572 3593 Fragments arranged by size: 3593 1572 1069 937 ApaLI G'TGCA_C Cuts at: 5228 6384 5228 Size: 1156 6015 ApoI r'AATT_y Cuts at: 1493 2257 2563 3378 3667 3739 4480 4706 4858 Size: 764 306 815 289 72 741 226 152 Cuts at: 4858 6967 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 815 764 741 306 289 226 152 72 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4496 1870 Size: 73 2553 4545 Fragments arranged by size: 4545 2553 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4427 4460 4427 Size: 33 7138 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3677 4492 5947 618 Size: 325 217 627 1890 815 1455 1842 Fragments arranged by size: 1890 1842 1455 815 627 325 217 BanII G_rGCy'C Cuts at: 1391 4490 1391 Size: 3099 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 3438 928 Size: 971 1474 65 4661 Fragments arranged by size: 4661 1474 971 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 5362 5396 1903 Size: 34 2000 34 1391 34 3678 Fragments arranged by size: 3678 2000 1391 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 3099 3109 3175 1548 Size: 1551 10 66 5544 Fragments arranged by size: 5544 1551 66 10 BfrBI ATG'CAT Cuts at: 3514 3514 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2244 5782 136 Size: 107 122 71 631 605 572 3538 1525 Fragments arranged by size: 3538 1525 631 605 572 122 107 71 BglII A'GATC_T Cuts at: 2300 7166 2300 Size: 4866 2305 BlpI GC'TnA_GC Cuts at: 4588 4588 Size: 7171 Bme1580I G_kGCm'C Cuts at: 1790 5232 6388 1790 Size: 3442 1156 2573 Fragments arranged by size: 3442 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3158 4902 5860 Size: 416 53 83 399 24 1741 1744 958 Cuts at: 5860 442 Size: 1753 Fragments arranged by size: 1753 1744 1741 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2359 2745 3659 5831 1239 Size: 1120 386 914 2172 2579 Fragments arranged by size: 2579 2172 1120 914 386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 5296 6161 6459 2774 Size: 2522 865 298 3486 Fragments arranged by size: 3486 2522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2804 5834 882 Size: 617 1305 3030 2219 Fragments arranged by size: 3030 2219 1305 617 BsaAI yAC'GTr Cuts at: 493 1204 2356 4962 493 Size: 711 1152 2606 2702 Fragments arranged by size: 2702 2606 1152 711 BsaBI GATnn'nnATC Cuts at: 3207 3234 3633 7165 3207 Size: 27 399 3532 3213 Fragments arranged by size: 3532 3213 399 27 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2294 4978 5360 5995 Size: 53 83 186 936 761 2684 382 635 Cuts at: 5995 275 Size: 1451 Fragments arranged by size: 2684 1451 936 761 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4150 5604 6002 6276 6423 6834 4150 Size: 1454 398 274 147 411 4487 Fragments arranged by size: 4487 1454 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3640 3670 3970 4000 1178 Size: 30 165 30 2237 30 300 30 4349 Fragments arranged by size: 4349 2237 300 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4059 1186 Size: 2873 4298 BseYI C'CCAG_C Cuts at: 6374 6870 6374 Size: 496 6675 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2095 1163 Size: 573 359 6239 Fragments arranged by size: 6239 573 359 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4621 5382 6410 1540 Size: 275 186 2620 761 1028 2301 Fragments arranged by size: 2620 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5232 5317 6388 5232 Size: 85 1071 6015 Fragments arranged by size: 6015 1071 85 BsmI GAATG_Cn' Cuts at: 3751 4682 4775 3751 Size: 931 93 6147 Fragments arranged by size: 6147 931 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4490 5232 5317 6388 1391 Size: 399 2700 742 85 1071 2174 Fragments arranged by size: 2700 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 4150 6834 4150 Size: 2684 4487 BspHI T'CATG_A Cuts at: 3136 5060 6790 3136 Size: 1924 1730 3517 Fragments arranged by size: 3517 1924 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4526 844 Size: 3682 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5058 1394 Size: 429 3235 3507 Fragments arranged by size: 3507 3235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3706 5666 5840 65 Size: 1183 2458 1960 174 1396 Fragments arranged by size: 2458 1960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5815 6002 1700 Size: 110 4005 187 2869 Fragments arranged by size: 4005 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 4411 794 Size: 3617 3554 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2300 3835 4501 4868 5253 5270 6722 6808 Size: 328 1535 666 367 385 17 1452 86 Cuts at: 6808 6820 7166 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1535 1452 666 385 367 346 328 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5680 Fragments arranged by size: 5680 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 2874 4278 4382 4687 5481 5501 2874 Size: 1404 104 305 794 20 4544 Fragments arranged by size: 4544 1404 794 305 104 20 ClaI AT'CG_AT Cuts at: 1554 2043 1554 Size: 489 6682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2703 2738 529 Size: 35 2139 35 4962 Fragments arranged by size: 4962 2139 35 35 DraI TTT'AAA Cuts at: 2878 3402 3672 4092 4475 2878 Size: 524 270 420 383 5574 Fragments arranged by size: 5574 524 420 383 270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6178 809 Size: 507 4862 1802 Fragments arranged by size: 4862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 3550 4618 Size: 54 1425 50 275 18 406 1314 1068 Cuts at: 4618 5507 7136 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1314 1068 889 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3759 4089 5101 1353 Size: 2406 330 1012 3423 Fragments arranged by size: 3423 2406 1012 330 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5758 6132 6278 387 Size: 1064 138 4169 374 146 1280 Fragments arranged by size: 4169 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2208 2359 2745 3450 3659 5234 Size: 393 564 12 151 386 705 209 1575 Cuts at: 5234 5831 6618 6886 1239 Size: 597 787 268 1524 Fragments arranged by size: 1575 1524 787 705 597 564 393 386 268 209 151 12 EcoO109I rG'GnC_Cy Cuts at: 2765 2765 Size: 7171 EcoRI G'AATT_C Cuts at: 2563 4480 4858 2563 Size: 1917 378 4876 Fragments arranged by size: 4876 1917 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2987 2987 Size: 7171 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 6318 1678 Size: 4640 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 5812 5844 5886 Size: 32 135 32 464 32 3744 32 42 Cuts at: 5886 5918 1373 Size: 32 2626 Fragments arranged by size: 3744 2626 464 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3067 3532 4132 4515 4761 669 Size: 988 281 1129 465 600 383 246 3079 Fragments arranged by size: 3079 1129 988 600 465 383 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7171 HpaI GTT'AAC Cuts at: 4132 4761 4132 Size: 629 6542 KpnI G_GTAC'C Cuts at: 4496 4496 Size: 7171 MfeI C'AATT_G Cuts at: 2697 4770 2697 Size: 2073 5098 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2982 3510 3925 6285 6469 1305 Size: 1677 528 415 2360 184 2007 Fragments arranged by size: 2360 2007 1677 528 415 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 3552 10 Size: 54 1425 343 406 1314 3629 Fragments arranged by size: 3629 1425 1314 406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3402 3762 4400 5129 5488 5647 518 Size: 648 2236 360 638 729 359 159 2042 Fragments arranged by size: 2236 2042 729 648 638 360 359 159 MspA1I CmG'CkG Cuts at: 1606 4587 5264 6412 6657 1606 Size: 2981 677 1148 245 2120 Fragments arranged by size: 2981 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5680 Fragments arranged by size: 5680 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NsiI A_TGCA'T Cuts at: 3516 3516 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2276 2429 2616 4028 4403 4529 6074 Size: 97 1047 153 187 1412 375 126 1545 Cuts at: 6074 1132 Size: 2229 Fragments arranged by size: 2229 1545 1412 1047 375 187 153 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7171 PciI A'CATG_T Cuts at: 2612 4399 6070 2612 Size: 1787 1671 3713 Fragments arranged by size: 3713 1787 1671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7171 PfoI T'CCnGG_A Cuts at: 3802 3802 Size: 7171 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PstI C_TGCA'G Cuts at: 830 4523 830 Size: 3693 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SapI GCTCTTCn'nnn_ Cuts at: 3759 3759 Size: 7171 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 4428 5419 7131 4428 Size: 991 1712 4468 Fragments arranged by size: 4468 1712 991 SfcI C'TryA_G Cuts at: 826 1045 4519 5654 6335 6526 826 Size: 219 3474 1135 681 191 1471 Fragments arranged by size: 3474 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4498 1872 Size: 2626 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 5275 6176 6438 820 Size: 197 926 810 2522 901 262 1553 Fragments arranged by size: 2522 1553 926 901 810 262 197 SnaBI TAC'GTA Cuts at: 493 2356 4962 493 Size: 1863 2606 2702 Fragments arranged by size: 2702 2606 1863 SpeI A'CTAG_T Cuts at: 152 3092 152 Size: 2940 4231 SphI G_CATG'C Cuts at: 1132 2276 4529 1132 Size: 1144 2253 3774 Fragments arranged by size: 3774 2253 1144 SspI AAT'ATT Cuts at: 5 52 2470 3478 3524 5095 5 Size: 47 2418 1008 46 1571 2081 Fragments arranged by size: 2418 2081 1571 1008 47 46 StuI AGG'CCT Cuts at: 1517 1517 Size: 7171 StyI C'CwwG_G Cuts at: 513 1065 2004 3129 4290 4610 513 Size: 552 939 1125 1161 320 3074 Fragments arranged by size: 3074 1161 1125 939 552 320 SwaI ATTT'AAAT Cuts at: 3402 3672 3402 Size: 270 6901 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5198 5215 5368 5553 5998 325 Size: 1255 3618 17 153 185 445 1498 Fragments arranged by size: 3618 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3413 3854 4426 Size: 274 80 33 51 1443 1436 441 572 Cuts at: 4426 5417 7129 96 Size: 991 1712 138 Fragments arranged by size: 1712 1443 1436 991 572 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3798 3830 7131 7163 3798 Size: 32 3301 32 3806 Fragments arranged by size: 3806 3301 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7171 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2246 2246 Size: 7171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 4496 1870 Size: 2626 4545 XmnI GAAnn'nnTTC Cuts at: 5300 5300 Size: 7171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI