(Circular) (Six-base) MAPSORT of: pFN21AB6547.seq Check: 9532 from: 1 to: 5815 pFN21AB6547 5815 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 3878 5262 5530 1632 Size: 1244 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1244 1002 268 AflII C'TTAA_G Cuts at: 820 1017 2993 820 Size: 197 1976 3642 Fragments arranged by size: 3642 1976 197 AflIII A'CryG_T Cuts at: 1203 2440 4714 5691 1203 Size: 1237 2274 977 1327 Fragments arranged by size: 2274 1327 1237 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 3558 5130 3558 Size: 1572 4243 ApaLI G'TGCA_C Cuts at: 2151 3872 5028 2151 Size: 1721 1156 2938 Fragments arranged by size: 2938 1721 1156 ApoI r'AATT_y Cuts at: 1493 2143 2240 2309 2323 2658 3124 3350 3502 Size: 650 97 69 14 335 466 226 152 Cuts at: 3502 5611 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 650 466 335 226 152 97 69 14 AseI AT'TA_AT Cuts at: 160 1032 2666 4369 160 Size: 872 1634 1703 1606 Fragments arranged by size: 1703 1634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2052 3140 1870 Size: 73 109 1088 4545 Fragments arranged by size: 4545 1088 109 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3136 4591 618 Size: 325 217 627 1349 1455 1842 Fragments arranged by size: 1842 1455 1349 627 325 217 BanII G_rGCy'C Cuts at: 1391 2947 3134 1391 Size: 1556 187 4072 Fragments arranged by size: 4072 1556 187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 2682 928 Size: 971 151 632 4061 Fragments arranged by size: 4061 971 632 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 2974 1548 Size: 1426 4389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4426 136 Size: 107 122 71 631 605 2754 1525 Fragments arranged by size: 2754 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2155 3876 5032 1790 Size: 365 1721 1156 2573 Fragments arranged by size: 2573 1721 1156 365 BmgBI CAC'GTC Cuts at: 1318 2068 1318 Size: 750 5065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2037 2199 2728 Size: 416 53 83 399 24 620 162 529 Cuts at: 2728 3546 4504 442 Size: 818 958 1753 Fragments arranged by size: 1753 958 818 620 529 416 399 162 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4475 1239 Size: 3236 2579 Bpu10I CC'TnA_GC Cuts at: 3003 3003 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2931 3940 4805 5103 2931 Size: 1009 865 298 3643 Fragments arranged by size: 3643 1009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2761 3606 493 Size: 711 1557 845 2702 Fragments arranged by size: 2702 1557 845 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3622 4004 4639 275 Size: 53 83 186 936 2089 382 635 1451 Fragments arranged by size: 2089 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2412 1186 Size: 1226 4589 BseYI C'CCAG_C Cuts at: 5018 5514 5018 Size: 496 5319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2155 2947 3876 3961 5032 2155 Size: 792 929 85 1071 2938 Fragments arranged by size: 2938 1071 929 792 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2947 3134 3876 3961 5032 1391 Size: 399 365 792 187 742 85 1071 2174 Fragments arranged by size: 2174 1071 792 742 399 365 187 85 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2527 3170 844 Size: 1683 643 3489 Fragments arranged by size: 3489 1683 643 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 2159 96 Size: 2063 3752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 3098 794 Size: 2304 3511 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5815 BstYI r'GATC_y Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810 Size: 1173 367 385 17 1452 86 12 346 Cuts at: 5810 1972 Size: 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2622 2622 Size: 5815 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 3331 4125 4145 3331 Size: 794 20 5001 Fragments arranged by size: 5001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 2643 3119 2643 Size: 476 5339 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 3262 4151 Size: 54 1425 50 275 18 622 810 889 Cuts at: 4151 5780 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 810 622 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2945 2945 Size: 5815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2876 3878 4475 5262 5530 1239 Size: 393 1244 1002 597 787 268 1524 Fragments arranged by size: 1524 1244 1002 787 597 393 268 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 EcoRV GAT'ATC Cuts at: 2122 2122 Size: 5815 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 4962 1678 Size: 3284 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373 Size: 32 135 32 2884 32 42 32 2626 Fragments arranged by size: 2884 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 2880 3414 2880 Size: 534 5281 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2441 4929 5113 1305 Size: 756 380 2488 184 2007 Fragments arranged by size: 2488 2007 756 380 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2454 10 Size: 54 1425 343 622 3371 Fragments arranged by size: 3371 1425 622 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 3773 4132 4291 518 Size: 648 1264 1343 359 159 2042 Fragments arranged by size: 2042 1343 1264 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3231 3908 5056 5301 1606 Size: 1625 677 1148 245 2120 Fragments arranged by size: 2120 1625 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5263 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2444 3173 4718 1132 Size: 97 1215 729 1545 2229 Fragments arranged by size: 2229 1545 1215 729 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 2440 4714 2440 Size: 2274 3541 PflMI CCAn_nnn'nTGG Cuts at: 1108 2591 1108 Size: 1483 4332 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspXI vC'TCGA_Gb Cuts at: 2052 2052 Size: 5815 PstI C_TGCA'G Cuts at: 830 3167 830 Size: 2337 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 SacI G_AGCT'C Cuts at: 2947 2947 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2814 3163 4298 4979 5170 826 Size: 219 1769 349 1135 681 191 1471 Fragments arranged by size: 1769 1471 1135 681 349 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2052 2946 2993 3919 4820 5082 Size: 197 926 109 894 47 926 901 262 Cuts at: 5082 820 Size: 1553 Fragments arranged by size: 1553 926 926 901 894 262 197 109 47 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 2580 2839 3739 5 Size: 47 2528 259 900 2081 Fragments arranged by size: 2528 2081 900 259 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 3254 513 Size: 552 2189 3074 Fragments arranged by size: 3074 2189 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 3859 4012 4197 4642 325 Size: 1255 2262 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2159 2830 4061 Size: 274 80 33 51 1443 182 671 1231 Cuts at: 4061 5773 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1231 671 274 182 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2398 2430 2460 2492 5775 5807 2398 Size: 32 30 32 3283 32 2406 Fragments arranged by size: 3283 2406 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 2052 1943 Size: 109 5706 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 2145 3944 2145 Size: 1799 4016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI