################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 2220 GCGG CCGC(-3/-1) AhdI 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1762 ATTAAT AT^TAAT AsnI 1762 ATTAAT AT^TAAT AspEI 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2213 CCCGAG C^[CT]CG[GA]G AvrII 469 CCTAGG C^CTAGG BcgI 2162 GCAAGTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 469 CCTAGG C^CTAGG BlpI 124 GCTAAGC GC^T[ACGT]AGC Bpu1102I 124 GCTAAGC GC^T[ACGT]AGC BsgI 1416 GTGCAG GTGCAG(16/14) BsoBI 2213 CCCGAG C^[CT]CG[GA]G Bsp1407I 1102 TGTACA T^GTACA BspHI 1116 TCATGA T^CATGA BsrFI 2252 GCCGGC [GA]^CCGG[CT] BsrGI 1102 TGTACA T^GTACA BstAPI 764 GCAAGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 124 GCTAAGC GC^T[ACGT]AGC Cfr10I 2252 GCCGGC [GA]^CCGG[CT] DraI 1894 TTTAAA TTT^AAA DraII 627 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1556 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2134 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1518 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 627 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 372 GAATTC G^AATTC HgaI 298 GACGC GACGC(5/10) MaeII 205 ACGT A^CGT NaeI 2252 GCCGGC GCC^GGC NgoMIV 2252 GCCGGC G^CCGGC PflFI 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 627 GGGACCC [GA]G^G[AT]CC[CT] PshBI 1762 ATTAAT AT^TAAT PstI 390 CTGCAG CTGCA^G RcaI 1116 TCATGA T^CATGA SapI 187 GCTCTTC GCTCTTC(1/4) SexAI 1754 ACCAGGT A^CC[AT]GGT SfcI 390 CTGCAG C^T[GA][CT]AG SmlI 1342 CTCAAG C^T[CT][GA]AG SspBI 1102 TGTACA T^GTACA TaqI 1968 TCGA T^CGA Tth111I 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1968 TCGA T^CGA XcmI 239 CCAAGTTCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 1970 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2071 GAATTTCTTC AspHI 610 GAGCAC G[AT]GC[AT]^C 2149 GTGCTC BmyI 610 GAGCAC G[AGT]GC[ACT]^C 2149 GTGCTC BsaI 829 GAGACC GGTCTC(1/5) 1940 GGTCTC BsiHKAI 610 GAGCAC G[AT]GC[AT]^C 2149 GTGCTC Bsp1286I 610 GAGCAC G[AGT]GC[ACT]^C 2149 GTGCTC BstEII 552 GGTCACC G^GT[ACGT]ACC 1842 GGTAACC BstPI 552 GGTCACC G^GT[ACGT]ACC 1842 GGTAACC BstYI 739 AGATCC [GA]^GATC[CT] 1336 GGATCT Cac8I 773 GCAAGC GC[ACGT]^[ACGT]GC 2252 GCCGGC EarI 188 CTCTTC CTCTTC(1/4) 2223 GAAGAG EcoO65I 552 GGTCACC G^GT[ACGT]ACC 1842 GGTAACC HapII 607 CCGG C^CGG 2253 CCGG HpaII 607 CCGG C^CGG 2253 CCGG Ksp632I 188 CTCTTC CTCTTC(1/4) 2223 GAAGAG MflI 739 AGATCC [GA]^GATC[CT] 1336 GGATCT MspA1I 1492 CAGCTG C[AC]G^C[GT]G 1837 CAGCTG MspI 607 CCGG C^CGG 2253 CCGG NspI 1700 ACATGC [GA]CATG^[CT] 2207 ACATGC PvuII 1492 CAGCTG CAG^CTG 1837 CAGCTG ScaI 268 AGTACT AGT^ACT 2156 AGTACT SfaNI 1789 GATGC GCATC(5/9) 906 GCATC XhoII 739 AGATCC [GA]^GATC[CT] 1336 GGATCT XmnI 1970 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2071 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI BsrI BssKI BstF5I BstNI BstXI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI