(Circular) (Six-base) MAPSORT of: pFN21AE6716.seq Check: 7360 from: 1 to: 6970 pFN21AE6716 6970 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4780 278 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4313 1937 Size: 2376 4594 Acc65I G'GTAC_C Cuts at: 4291 4291 Size: 6970 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2740 4067 4071 5033 6417 6685 1632 Size: 1108 1327 4 962 1384 268 1917 Fragments arranged by size: 1917 1384 1327 1108 962 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6773 AflIII A'CryG_T Cuts at: 1203 5869 6846 1203 Size: 4666 977 1327 Fragments arranged by size: 4666 1327 977 AgeI A'CCGG_T Cuts at: 5801 5801 Size: 6970 AhdI GACnn_n'nnGTC Cuts at: 3195 5699 3195 Size: 2504 4466 AleI CACnn'nnGTG Cuts at: 1166 2232 2552 1166 Size: 1066 320 5584 Fragments arranged by size: 5584 1066 320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 2426 3173 3251 4140 4713 6285 2426 Size: 747 78 889 573 1572 3111 Fragments arranged by size: 3111 1572 889 747 573 78 ApaLI G'TGCA_C Cuts at: 5027 6183 5027 Size: 1156 5814 ApoI r'AATT_y Cuts at: 1493 2022 2377 4076 4279 4505 4657 6766 1493 Size: 529 355 1699 203 226 152 2109 1697 Fragments arranged by size: 2109 1699 1697 529 355 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 3768 5524 160 Size: 872 2736 1756 1606 Fragments arranged by size: 2736 1756 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 4218 4295 1870 Size: 73 2275 77 4545 Fragments arranged by size: 4545 2275 77 73 AvrII C'CTAG_G Cuts at: 2474 2474 Size: 6970 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3252 3285 3670 3703 3252 Size: 33 385 33 6519 Fragments arranged by size: 6519 385 33 33 BamHI G'GATC_C Cuts at: 4300 4667 4300 Size: 367 6603 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4291 5746 618 Size: 325 217 627 2504 1455 1842 Fragments arranged by size: 2504 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4289 1391 Size: 2898 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3615 3902 4215 928 Size: 971 1716 287 313 3683 Fragments arranged by size: 3683 1716 971 313 287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 5161 5195 1903 Size: 34 2219 34 971 34 3678 Fragments arranged by size: 3678 2219 971 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4862 6072 4862 Size: 1210 5760 BclI T'GATC_A Cuts at: 1548 1548 Size: 6970 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5581 136 Size: 107 122 71 631 605 3909 1525 Fragments arranged by size: 3909 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6965 6965 Size: 6970 BlpI GC'TnA_GC Cuts at: 2130 4387 2130 Size: 2257 4713 Bme1580I G_kGCm'C Cuts at: 1790 5031 6187 1790 Size: 3241 1156 2573 Fragments arranged by size: 3241 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6970 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2082 2829 4701 Size: 416 53 83 399 24 665 747 1872 Cuts at: 4701 5659 442 Size: 958 1753 Fragments arranged by size: 1872 1753 958 747 665 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6970 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3273 3425 3465 4142 5630 1239 Size: 2034 152 40 677 1488 2579 Fragments arranged by size: 2579 2034 1488 677 152 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3332 5095 5960 6258 3332 Size: 1763 865 298 4044 Fragments arranged by size: 4044 1763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2828 3951 5633 882 Size: 617 1329 1123 1682 2219 Fragments arranged by size: 2219 1682 1329 1123 617 BsaAI yAC'GTr Cuts at: 493 1204 4761 493 Size: 711 3557 2702 Fragments arranged by size: 3557 2702 711 BsaBI GATnn'nnATC Cuts at: 6964 6964 Size: 6970 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4777 5159 5794 275 Size: 53 83 186 936 3244 382 635 1451 Fragments arranged by size: 3244 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5403 5801 6075 6222 6633 5403 Size: 398 274 147 411 5740 Fragments arranged by size: 5740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4137 4167 1178 Size: 30 165 30 2734 30 3981 Fragments arranged by size: 3981 2734 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2679 3347 1186 Size: 1128 365 668 4809 Fragments arranged by size: 4809 1128 668 365 BseYI C'CCAG_C Cuts at: 2134 2748 2793 2802 6173 6669 2134 Size: 614 45 9 3371 496 2435 Fragments arranged by size: 3371 2435 614 496 45 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3442 1163 Size: 573 1706 4691 Fragments arranged by size: 4691 1706 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4420 5181 6209 1540 Size: 275 186 2419 761 1028 2301 Fragments arranged by size: 2419 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 4158 5031 5116 6187 2619 Size: 1539 873 85 1071 3402 Fragments arranged by size: 3402 1539 1071 873 85 BsmI GAATG_Cn' Cuts at: 2118 3032 3357 4002 4481 4574 2118 Size: 914 325 645 479 93 4514 Fragments arranged by size: 4514 914 645 479 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2619 4158 4289 5031 5116 6187 1391 Size: 399 829 1539 131 742 85 1071 2174 Fragments arranged by size: 2174 1539 1071 829 742 399 131 85 BspEI T'CCGG_A Cuts at: 6633 6633 Size: 6970 BspHI T'CATG_A Cuts at: 3121 4859 6589 3121 Size: 1738 1730 3502 Fragments arranged by size: 3502 1738 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4325 844 Size: 3481 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4857 1394 Size: 429 3034 3507 Fragments arranged by size: 3507 3034 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5465 5639 65 Size: 1183 4217 174 1396 Fragments arranged by size: 4217 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4257 5614 5801 1700 Size: 110 2447 1357 187 2869 Fragments arranged by size: 2869 2447 1357 187 110 BsrGI T'GTAC_A Cuts at: 96 3107 96 Size: 3011 3959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 5030 6042 5030 Size: 1012 5958 BstAPI GCAn_nnn'nTGC Cuts at: 794 2775 794 Size: 1981 4989 BstBI TT'CG_AA Cuts at: 5774 5774 Size: 6970 BstEII G'GTnAC_C Cuts at: 1184 2557 3847 1184 Size: 1373 1290 4307 Fragments arranged by size: 4307 1373 1290 BstXI CCAn_nnnn'nTGG Cuts at: 2151 2627 3050 2151 Size: 476 423 6071 Fragments arranged by size: 6071 476 423 BstYI r'GATC_y Cuts at: 1972 2744 3341 4300 4667 5052 5069 6521 6607 Size: 772 597 959 367 385 17 1452 86 Cuts at: 6607 6619 6965 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 959 772 597 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6418 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6231 Fragments arranged by size: 6231 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3061 3430 4486 5280 5300 2947 Size: 114 369 1056 794 20 4617 Fragments arranged by size: 4617 1056 794 369 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6970 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 3901 4274 3901 Size: 373 6597 DraIII CAC_nnn'GTG Cuts at: 3566 3566 Size: 6970 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4145 5977 809 Size: 507 2829 1832 1802 Fragments arranged by size: 2829 1832 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4417 5306 6935 Size: 54 1425 50 275 18 2587 889 1629 Cuts at: 6935 8 Size: 43 Fragments arranged by size: 2587 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4417 1537 Size: 275 2605 4090 Fragments arranged by size: 4090 2605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 4223 4900 1353 Size: 846 2024 677 3423 Fragments arranged by size: 3423 2024 846 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5557 5931 6077 387 Size: 1064 138 3968 374 146 1280 Fragments arranged by size: 3968 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2740 3273 3425 3465 4067 4071 4142 Size: 393 1108 533 152 40 602 4 71 Cuts at: 4142 5033 5630 6417 6685 1239 Size: 891 597 787 268 1524 Fragments arranged by size: 1524 1108 891 787 602 597 533 393 268 152 71 40 4 EcoNI CCTnn'n_nnAGG Cuts at: 3527 3527 Size: 6970 EcoO109I rG'GnC_Cy Cuts at: 2633 2633 Size: 6970 EcoRI G'AATT_C Cuts at: 2377 4279 4657 2377 Size: 1902 378 4690 Fragments arranged by size: 4690 1902 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3596 3596 Size: 6970 FspI TGC'GCA Cuts at: 5476 5476 Size: 6970 HaeII r_GCGC'y Cuts at: 1678 6117 1678 Size: 4439 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 2515 2547 2696 Size: 32 135 32 848 32 63 32 149 Cuts at: 2696 2728 2950 2982 3065 3097 3181 3213 3326 Size: 32 222 32 83 32 84 32 113 Cuts at: 3326 3358 5611 5643 5685 5717 1373 Size: 32 2253 32 42 32 2626 Fragments arranged by size: 2626 2253 848 222 149 135 113 84 83 63 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4314 4560 669 Size: 988 281 2376 246 3079 Fragments arranged by size: 3079 2376 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6970 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KpnI G_GTAC'C Cuts at: 4295 4295 Size: 6970 MfeI C'AATT_G Cuts at: 4569 4569 Size: 6970 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2680 6084 6268 1305 Size: 1375 3404 184 2007 Fragments arranged by size: 3404 2007 1375 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5148 Fragments arranged by size: 5148 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 2552 2625 3770 3956 4928 5287 Size: 648 1066 320 73 1145 186 972 359 Cuts at: 5287 5446 518 Size: 159 2042 Fragments arranged by size: 2042 1145 1066 972 648 359 320 186 159 73 MspA1I CmG'CkG Cuts at: 1606 3499 3844 4386 5063 6211 6456 1606 Size: 1893 345 542 677 1148 245 2120 Fragments arranged by size: 2120 1893 1148 677 542 345 245 NaeI GCC'GGC Cuts at: 4259 4259 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6418 NdeI CA'TA_TG Cuts at: 387 387 Size: 6970 NgoMIV G'CCGG_C Cuts at: 4257 4257 Size: 6970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6970 NotI GC'GGCC_GC Cuts at: 4417 4417 Size: 6970 NspI r_CATG'y Cuts at: 1132 1229 3709 4216 4328 5873 1132 Size: 97 2480 507 112 1545 2229 Fragments arranged by size: 2480 2229 1545 507 112 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6970 PciI A'CATG_T Cuts at: 5869 5869 Size: 6970 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6970 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2633 2633 Size: 6970 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6970 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PstI C_TGCA'G Cuts at: 830 2399 4322 830 Size: 1569 1923 3478 Fragments arranged by size: 3478 1923 1569 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 3499 3844 3499 Size: 345 6625 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SanDI GG'GwC_CC Cuts at: 2633 2633 Size: 6970 SapI GCTCTTCn'nnn_ Cuts at: 2199 2199 Size: 6970 SbfI CC_TGCA'GG Cuts at: 4322 4322 Size: 6970 ScaI AGT'ACT Cuts at: 2275 4163 5218 6930 2275 Size: 1888 1055 1712 2315 Fragments arranged by size: 2315 1888 1712 1055 SexAI A'CCwGG_T Cuts at: 3759 3759 Size: 6970 SfcI C'TryA_G Cuts at: 826 1045 2395 4318 5453 6134 6325 826 Size: 219 1350 1923 1135 681 191 1471 Fragments arranged by size: 1923 1471 1350 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 4297 1872 Size: 2425 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3347 5074 5975 6237 820 Size: 197 926 1404 1727 901 262 1553 Fragments arranged by size: 1727 1553 1404 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4761 493 Size: 4268 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SspI AAT'ATT Cuts at: 5 52 4894 5 Size: 47 4842 2081 Fragments arranged by size: 4842 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6970 StyI C'CwwG_G Cuts at: 513 1065 2474 2605 3414 3580 3997 4409 513 Size: 552 1409 131 809 166 417 412 3074 Fragments arranged by size: 3074 1409 809 552 417 412 166 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4997 5014 5167 5352 5797 325 Size: 1255 3417 17 153 185 445 1498 Fragments arranged by size: 3417 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 3107 4161 Size: 274 80 33 51 1443 296 834 1054 Cuts at: 4161 5216 6928 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 1054 834 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2303 2335 4137 4169 6930 6962 2303 Size: 32 1802 32 2761 32 2311 Fragments arranged by size: 2761 2311 1802 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3761 1316 Size: 2445 4525 XbaI T'CTAG_A Cuts at: 4306 4306 Size: 6970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2251 Size: 6970 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6970 XmaI C'CCGG_G Cuts at: 1870 4295 1870 Size: 2425 4545 XmnI GAAnn'nnTTC Cuts at: 3979 4080 5099 3979 Size: 101 1019 5850 Fragments arranged by size: 5850 1019 101 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4778 276 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI