(Linear) (Six-base) MAPSORT of: KIEE6716.seq Check: 3389 from: 1 to: 2265 KIEE6716.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 735 2062 2066 2265 Size: 735 1327 4 199 Fragments arranged by size: 1327 735 199 4 AhdI GACnn_n'nnGTC Cuts at: 0 1190 2265 Size: 1190 1075 AleI CACnn'nnGTG Cuts at: 0 227 547 2265 Size: 227 320 1718 Fragments arranged by size: 1718 320 227 AlwNI CAG_nnn'CTG Cuts at: 0 421 1168 1246 2135 2265 Size: 421 747 78 889 130 Fragments arranged by size: 889 747 421 130 78 ApoI r'AATT_y Cuts at: 0 17 372 2071 2265 Size: 17 355 1699 194 Fragments arranged by size: 1699 355 194 17 AseI AT'TA_AT Cuts at: 0 1763 2265 Size: 1763 502 AvaI C'yCGr_G Cuts at: 0 2213 2265 Size: 2213 52 AvrII C'CTAG_G Cuts at: 0 469 2265 Size: 469 1796 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1247 1280 1665 1698 2265 Size: 1247 33 385 33 567 Fragments arranged by size: 1247 567 385 33 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 1610 1897 2210 2265 Size: 1610 287 313 55 Fragments arranged by size: 1610 313 287 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2151 2185 2265 Size: 2151 34 80 Fragments arranged by size: 2151 80 34 BlpI GC'TnA_GC Cuts at: 0 125 2265 Size: 125 2140 BmrI ACTGGGnnnn_n' Cuts at: 0 77 824 2265 Size: 77 747 1441 Fragments arranged by size: 1441 747 77 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1268 1420 1460 2137 2265 Size: 1268 152 40 677 128 Fragments arranged by size: 1268 677 152 128 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1327 2265 Size: 1327 938 BsaI GGTCTCn'nnnn_ Cuts at: 0 823 1946 2265 Size: 823 1123 319 Fragments arranged by size: 1123 823 319 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2132 2162 2265 Size: 2132 30 103 Fragments arranged by size: 2132 103 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 309 674 1342 2265 Size: 309 365 668 923 Fragments arranged by size: 923 668 365 309 BseYI C'CCAG_C Cuts at: 0 129 743 788 797 2265 Size: 129 614 45 9 1468 Fragments arranged by size: 1468 614 129 45 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1437 2265 Size: 1437 828 BsiHKAI G_wGCw'C Cuts at: 0 614 2153 2265 Size: 614 1539 112 Fragments arranged by size: 1539 614 112 BsmI GAATG_Cn' Cuts at: 0 113 1027 1352 1997 2265 Size: 113 914 325 645 268 Fragments arranged by size: 914 645 325 268 113 Bsp1286I G_dGCh'C Cuts at: 0 614 2153 2265 Size: 614 1539 112 Fragments arranged by size: 1539 614 112 BspHI T'CATG_A Cuts at: 0 1116 2265 Size: 1116 1149 BsrFI r'CCGG_y Cuts at: 0 2252 2265 Size: 2252 13 BsrGI T'GTAC_A Cuts at: 0 1102 2265 Size: 1102 1163 BstAPI GCAn_nnn'nTGC Cuts at: 0 770 2265 Size: 770 1495 BstEII G'GTnAC_C Cuts at: 0 552 1842 2265 Size: 552 1290 423 Fragments arranged by size: 1290 552 423 BstXI CCAn_nnnn'nTGG Cuts at: 0 146 622 1045 2265 Size: 146 476 423 1220 Fragments arranged by size: 1220 476 423 146 BstYI r'GATC_y Cuts at: 0 739 1336 2265 Size: 739 597 929 Fragments arranged by size: 929 739 597 BtsI GCAGTG_nn' Cuts at: 0 942 1056 1425 2265 Size: 942 114 369 840 Fragments arranged by size: 942 840 369 114 DraI TTT'AAA Cuts at: 0 1896 2265 Size: 1896 369 DraIII CAC_nnn'GTG Cuts at: 0 1561 2265 Size: 1561 704 DrdI GACnn_nn'nnGTC Cuts at: 0 2140 2265 Size: 2140 125 EarI CTCTTCn'nnn_ Cuts at: 0 194 2218 2265 Size: 194 2024 47 Fragments arranged by size: 2024 194 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 735 1268 1420 1460 2062 2066 2137 2265 Size: 735 533 152 40 602 4 71 128 Fragments arranged by size: 735 602 533 152 128 71 40 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1522 2265 Size: 1522 743 EcoO109I rG'GnC_Cy Cuts at: 0 628 2265 Size: 628 1637 EcoRI G'AATT_C Cuts at: 0 372 2265 Size: 372 1893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1591 2265 Size: 1591 674 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 415 447 510 542 691 723 945 977 Size: 415 32 63 32 149 32 222 32 Cuts at: 977 1060 1092 1176 1208 1321 1353 2265 Size: 83 32 84 32 113 32 912 Fragments arranged by size: 912 415 222 149 113 84 83 63 32 32 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 675 2265 Size: 675 1590 MslI CAynn'nnrTG Cuts at: 0 227 547 620 1765 1951 2265 Size: 227 320 73 1145 186 314 Fragments arranged by size: 1145 320 314 227 186 73 MspA1I CmG'CkG Cuts at: 0 1494 1839 2265 Size: 1494 345 426 Fragments arranged by size: 1494 426 345 NaeI GCC'GGC Cuts at: 0 2254 2265 Size: 2254 11 NgoMIV G'CCGG_C Cuts at: 0 2252 2265 Size: 2252 13 NspI r_CATG'y Cuts at: 0 1704 2211 2265 Size: 1704 507 54 Fragments arranged by size: 1704 507 54 PpuMI rG'GwC_Cy Cuts at: 0 628 2265 Size: 628 1637 PstI C_TGCA'G Cuts at: 0 394 2265 Size: 394 1871 PvuII CAG'CTG Cuts at: 0 1494 1839 2265 Size: 1494 345 426 Fragments arranged by size: 1494 426 345 SanDI GG'GwC_CC Cuts at: 0 628 2265 Size: 628 1637 SapI GCTCTTCn'nnn_ Cuts at: 0 194 2265 Size: 194 2071 ScaI AGT'ACT Cuts at: 0 270 2158 2265 Size: 270 1888 107 Fragments arranged by size: 1888 270 107 SexAI A'CCwGG_T Cuts at: 0 1754 2265 Size: 1754 511 SfcI C'TryA_G Cuts at: 0 390 2265 Size: 390 1875 SmlI C'TyrA_G Cuts at: 0 1342 2265 Size: 1342 923 StyI C'CwwG_G Cuts at: 0 469 600 1409 1575 1992 2265 Size: 469 131 809 166 417 273 Fragments arranged by size: 809 469 417 273 166 131 TatI w'GTAC_w Cuts at: 0 268 1102 2156 2265 Size: 268 834 1054 109 Fragments arranged by size: 1054 834 268 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 298 330 2132 2164 2265 Size: 298 32 1802 32 101 Fragments arranged by size: 1802 298 101 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1756 2265 Size: 1756 509 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 246 2265 Size: 246 2019 XmnI GAAnn'nnTTC Cuts at: 0 1974 2075 2265 Size: 1974 101 190 Fragments arranged by size: 1974 190 101 Enzymes that do cut: AcuI AhdI AleI AlwNI ApoI AseI AvaI AvrII BaeI BbsI BcgI BlpI BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtsI DraI DraIII DrdI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MmeI MslI MspA1I NaeI NgoMIV NspI PpuMI PstI PvuII SanDI SapI ScaI SexAI SfcI SmlI StyI TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AsiSI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI EaeI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI ZraI