(Circular) (Six-base) MAPSORT of: pFN21AE9665.seq Check: 6197 from: 1 to: 6721 pFN21AE9665 6721 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3702 3714 4784 6168 6436 1632 Size: 896 1174 12 1070 1384 268 1917 Fragments arranged by size: 1917 1384 1174 1070 896 268 12 AfeI AGC'GCT Cuts at: 3708 3708 Size: 6721 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6524 AflIII A'CryG_T Cuts at: 1203 5620 6597 1203 Size: 4417 977 1327 Fragments arranged by size: 4417 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 2369 2399 3002 3272 1166 Size: 1203 30 603 270 4615 Fragments arranged by size: 4615 1203 603 270 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3693 1184 Size: 2509 4212 AlwNI CAG_nnn'CTG Cuts at: 2089 2536 4464 6036 2089 Size: 447 1928 1572 2774 Fragments arranged by size: 2774 1928 1572 447 ApaLI G'TGCA_C Cuts at: 3502 4778 5934 3502 Size: 1276 1156 4289 Fragments arranged by size: 4289 1276 1156 ApoI r'AATT_y Cuts at: 1493 2211 2303 2783 2950 3090 3314 3714 4030 Size: 718 92 480 167 140 224 400 316 Cuts at: 4030 4256 4408 6517 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 718 480 400 316 226 224 167 152 140 92 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 4046 1870 Size: 73 2103 4545 Fragments arranged by size: 4545 2103 73 BamHI G'GATC_C Cuts at: 3522 3543 4051 4418 3522 Size: 21 508 367 5825 Fragments arranged by size: 5825 508 367 21 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3461 4042 5497 618 Size: 325 217 627 266 1408 581 1455 1842 Fragments arranged by size: 1842 1455 1408 627 581 325 266 217 BanII G_rGCy'C Cuts at: 1391 2499 4040 1391 Size: 1108 1541 4072 Fragments arranged by size: 4072 1541 1108 BbeI G_GCGC'C Cuts at: 2057 3465 2057 Size: 1408 5313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2718 928 Size: 971 819 4931 Fragments arranged by size: 4931 971 819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5823 4613 Size: 1210 5511 BclI T'GATC_A Cuts at: 1548 1548 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 5332 136 Size: 107 122 71 631 605 748 2912 1525 Fragments arranged by size: 2912 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 6716 6716 Size: 6721 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 3506 4782 5938 1790 Size: 1716 1276 1156 2573 Fragments arranged by size: 2573 1716 1276 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3920 4452 5410 Size: 416 53 83 399 24 2503 532 958 Cuts at: 5410 442 Size: 1753 Fragments arranged by size: 2503 1753 958 532 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2120 5381 1239 Size: 818 63 3261 2579 Fragments arranged by size: 3261 2579 818 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 2445 4846 5711 6009 2354 Size: 91 2401 865 298 3066 Fragments arranged by size: 3066 2401 865 298 91 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3013 3035 5384 882 Size: 617 1514 22 2349 2219 Fragments arranged by size: 2349 2219 1514 617 22 BsaAI yAC'GTr Cuts at: 493 1204 4512 493 Size: 711 3308 2702 Fragments arranged by size: 3308 2702 711 BsaBI GATnn'nnATC Cuts at: 6715 6715 Size: 6721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2093 3462 4528 Size: 53 83 186 936 521 39 1369 1066 Cuts at: 4528 4910 5545 275 Size: 382 635 1451 Fragments arranged by size: 1451 1369 1066 936 635 521 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 3404 5154 5552 5826 5973 6384 3404 Size: 1750 398 274 147 411 3741 Fragments arranged by size: 3741 1750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2504 2534 3399 Size: 30 165 30 675 30 396 30 865 Cuts at: 3399 3429 1178 Size: 30 4470 Fragments arranged by size: 4470 865 675 396 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2638 2657 2783 3001 1186 Size: 1452 19 126 218 4906 Fragments arranged by size: 4906 1452 218 126 19 BseYI C'CCAG_C Cuts at: 2070 5924 6420 2070 Size: 3854 496 2371 Fragments arranged by size: 3854 2371 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3265 1163 Size: 573 1529 4619 Fragments arranged by size: 4619 1529 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3405 4171 4932 5960 1540 Size: 275 186 1404 766 761 1028 2301 Fragments arranged by size: 2301 1404 1028 766 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2471 2499 3506 4782 4867 5938 2471 Size: 28 1007 1276 85 1071 3254 Fragments arranged by size: 3254 1276 1071 1007 85 28 BsmI GAATG_Cn' Cuts at: 4232 4325 4232 Size: 93 6628 BsmBI CGTCTCn'nnnn_ Cuts at: 2100 3392 2100 Size: 1292 5429 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2471 2499 3506 4040 4782 4867 5938 Size: 399 681 28 1007 534 742 85 1071 Cuts at: 5938 1391 Size: 2174 Fragments arranged by size: 2174 1071 1007 742 681 534 399 85 28 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 4610 6340 4610 Size: 1730 4991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3675 4076 844 Size: 2831 401 3489 Fragments arranged by size: 3489 2831 401 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3192 5216 5390 65 Size: 1183 1944 2024 174 1396 Fragments arranged by size: 2024 1944 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 2521 794 Size: 1727 4994 BstBI TT'CG_AA Cuts at: 3135 5525 3135 Size: 2390 4331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 2346 3022 3071 3211 2346 Size: 676 49 140 5856 Fragments arranged by size: 5856 676 140 49 BstYI r'GATC_y Cuts at: 1972 3522 3543 3754 4051 4418 4803 4820 6272 Size: 1550 21 211 297 367 385 17 1452 Cuts at: 6272 6358 6370 6716 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1550 1452 385 367 346 297 211 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2478 2847 3358 3594 513 Size: 552 939 45 429 369 511 236 3640 Fragments arranged by size: 3640 939 552 511 429 369 236 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 2140 2430 3398 3529 4237 5031 5051 2140 Size: 290 968 131 708 794 20 3810 Fragments arranged by size: 3810 968 794 708 290 131 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 2790 4025 2790 Size: 1235 5486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3163 4168 Size: 54 1425 50 275 18 216 1117 1005 Cuts at: 4168 5057 6686 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1117 1005 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 3756 4651 1353 Size: 1281 1122 895 3423 Fragments arranged by size: 3423 1281 1122 895 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5308 5682 5828 387 Size: 1064 138 3719 374 146 1280 Fragments arranged by size: 3719 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2497 2497 Size: 6721 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2120 2528 3702 3714 4784 5381 Size: 393 425 63 408 1174 12 1070 597 Cuts at: 5381 6168 6436 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1174 1070 787 597 425 408 393 268 63 12 EcoNI CCTnn'n_nnAGG Cuts at: 3551 3551 Size: 6721 EcoO109I rG'GnC_Cy Cuts at: 2329 2329 Size: 6721 EcoRI G'AATT_C Cuts at: 2303 2950 4030 4408 2303 Size: 647 1080 378 4616 Fragments arranged by size: 4616 1080 647 378 EcoRV GAT'ATC Cuts at: 3591 3591 Size: 6721 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3577 3577 Size: 6721 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 2057 3465 3710 5868 1678 Size: 379 1408 245 2158 2531 Fragments arranged by size: 2531 2158 1408 379 245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 3013 3045 3098 Size: 32 135 32 506 32 903 32 53 Cuts at: 3098 3130 5362 5394 5436 5468 1373 Size: 32 2232 32 42 32 2626 Fragments arranged by size: 2626 2232 903 506 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2740 4065 4311 669 Size: 988 281 802 1325 246 3079 Fragments arranged by size: 3079 1325 988 802 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KasI G'GCGC_C Cuts at: 2053 3461 2053 Size: 1408 5313 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 2586 4320 2586 Size: 1734 4987 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3192 5835 6019 1305 Size: 1887 2643 184 2007 Fragments arranged by size: 2643 2007 1887 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3165 10 Size: 54 1425 343 1333 3566 Fragments arranged by size: 3566 1425 1333 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2344 2367 2369 2399 3002 3020 3272 Size: 648 1178 23 2 30 603 18 252 Cuts at: 3272 3618 4679 5038 5197 518 Size: 346 1061 359 159 2042 Fragments arranged by size: 2042 1178 1061 648 603 359 346 252 159 30 23 18 2 MspA1I CmG'CkG Cuts at: 1606 2045 2312 2985 4137 4814 5962 6207 1606 Size: 439 267 673 1152 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 677 673 439 267 245 NarI GG'CG_CC Cuts at: 2054 3462 2054 Size: 1408 5313 NcoI C'CATG_G Cuts at: 513 1065 2004 2049 2478 2847 3594 513 Size: 552 939 45 429 369 747 3640 Fragments arranged by size: 3640 939 747 552 429 369 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6721 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 2597 4079 5624 1132 Size: 97 1368 1482 1545 2229 Fragments arranged by size: 2229 1545 1482 1368 97 PasI CC'CwG_GG Cuts at: 1348 3491 1348 Size: 2143 4578 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 3172 1108 Size: 2064 4657 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2433 2465 4790 4822 6303 6335 2433 Size: 32 2325 32 1481 32 2819 Fragments arranged by size: 2819 2325 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 2329 Size: 6721 PshAI GACnn'nnGTC Cuts at: 1613 3407 1613 Size: 1794 4927 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PstI C_TGCA'G Cuts at: 830 2532 3393 4073 830 Size: 1702 861 680 3478 Fragments arranged by size: 3478 1702 861 680 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 2312 2312 Size: 6721 SacI G_AGCT'C Cuts at: 2499 2499 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SapI GCTCTTCn'nnn_ Cuts at: 3756 3756 Size: 6721 SbfI CC_TGCA'GG Cuts at: 2532 4073 2532 Size: 1541 5180 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SexAI A'CCwGG_T Cuts at: 2158 2158 Size: 6721 SfcI C'TryA_G Cuts at: 826 1045 2528 3333 3389 4069 5204 5885 6076 Size: 219 1483 805 56 680 1135 681 191 Cuts at: 6076 826 Size: 1471 Fragments arranged by size: 1483 1471 1135 805 681 680 219 191 56 SfoI GGC'GCC Cuts at: 2055 3463 2055 Size: 1408 5313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 4048 1872 Size: 2176 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 2460 4825 5726 5988 820 Size: 197 926 390 127 2365 901 262 1553 Fragments arranged by size: 2365 1553 926 901 390 262 197 127 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 4079 1132 Size: 2947 3774 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 2288 1517 Size: 771 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2478 2847 3465 3594 4160 Size: 552 939 45 429 369 618 129 566 Cuts at: 4160 513 Size: 3074 Fragments arranged by size: 3074 939 618 566 552 429 369 129 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2530 2730 4748 4765 4918 5103 5548 Size: 1255 950 200 2018 17 153 185 445 Cuts at: 5548 325 Size: 1498 Fragments arranged by size: 2018 1498 1255 950 445 200 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4967 6679 96 Size: 274 80 33 51 1443 2990 1712 138 Fragments arranged by size: 2990 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3399 3431 6681 6713 3399 Size: 32 3250 32 3407 Fragments arranged by size: 3407 3250 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 3757 4057 3757 Size: 300 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3592 3592 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 4046 1870 Size: 2176 4545 XmnI GAAnn'nnTTC Cuts at: 3977 4850 3977 Size: 873 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI