################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI Ksp632I KspI MamI MboI MflI MluI MroI MvnI NarI NciI NdeII NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 548 GTCTAC GT^[AC][GT]AC AciI 389 CCGC CCGC(-3/-1) AflII 471 CTTAAG C^TTAAG Alw44I 1100 GTGCAC G^TGCAC ApaLI 1100 GTGCAC G^TGCAC AseI 903 ATTAAT AT^TAAT AsnI 903 ATTAAT AT^TAAT AspHI 1100 GTGCAC G[AT]GC[AT]^C AvrII 774 CCTAGG C^CTAGG BalI 446 TGGCCA TGG^CCA BbsI 1329 GAAGAC GAAGAC(2/6) BfrI 471 CTTAAG C^TTAAG BglI 562 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 774 CCTAGG C^CTAGG BpuAI 1329 GAAGAC GAAGAC(2/6) BsaI 585 GAGACC GGTCTC(1/5) BseRI 1169 CTCCTC GAGGAG(10/8) BsiEI 256 CGGCCG CG[GA][CT]^CG BsiHKAI 1100 GTGCAC G[AT]GC[AT]^C BsmAI 585 GAGAC GTCTC(1/5) Bsp1407I 1217 TGTACA T^GTACA BspHI 581 TCATGA T^CATGA BsrDI 1271 GCAATG GCAATG(2/0) BsrGI 1217 TGTACA T^GTACA BssKI 1017 CCTGG ^CC[ACGT]GG BssSI 1093 CTCGTG CACGAG(-5/-1) BstNI 1017 CCTGG CC^[AT]GG BstXI 188 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 216 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1400 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 256 CGGCCG C^GGCCG EclXI 256 CGGCCG C^GGCCG Eco52I 256 CGGCCG C^GGCCG EcoO109I 216 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 935 GAATTC G^AATTC EcoRII 1017 CCTGG ^CC[AT]GG EcoT22I 101 ATGCAT ATGCA^T FauI 388 CCCGC CCCGC(4/6) HincII 1378 GTTAAC GT[CT]^[GA]AC HindII 1378 GTTAAC GT[CT]^[GA]AC HindIII 528 AAGCTT A^AGCTT HpaI 1378 GTTAAC GTT^AAC MaeII 1382 ACGT A^CGT MfeI 1136 CAATTG C^AATTG MluNI 446 TGGCCA TGG^CCA MscI 446 TGGCCA TGG^CCA MspA1I 331 CAGCTG C[AC]G^C[GT]G MunI 1136 CAATTG C^AATTG MvaI 1017 CCTGG CC^[AT]GG NaeI 254 GCCGGC GCC^GGC NdeI 809 CATATG CA^TATG NgoMIV 254 GCCGGC G^CCGGC NsiI 101 ATGCAT ATGCA^T PpuMI 216 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 903 ATTAAT AT^TAAT PvuII 331 CAGCTG CAG^CTG RcaI 581 TCATGA T^CATGA ScrFI 1017 CCTGG CC^[ACGT]GG SfaNI 30 GCATC GCATC(5/9) SspBI 1217 TGTACA T^GTACA SspI 147 AATATT AAT^ATT StuI 889 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 137 AAATTC [GA]^AATT[CT] 935 GAATTC AflIII 1214 ACATGT A^C[GA][CT]GT 1220 ACATGT ApoI 137 AAATTC [GA]^AATT[CT] 935 GAATTC BfaI 775 CTAG C^TAG 887 CTAG BpmI 38 CTCCAG CTGGAG(16/14) 1199 CTGGAG BsmI 558 GAATGC GAATGC(1/-1) 103 GCATTC BspLU11I 1214 ACATGT A^CATGT 1220 ACATGT BspMI 380 ACCTGC ACCTGC(4/8) 1177 GCAGGT BsrFI 254 GCCGGC [GA]^CCGG[CT] 258 GCCGGT Cfr10I 254 GCCGGC [GA]^CCGG[CT] 258 GCCGGT DdeI 614 CTAAG C^T[ACGT]AG 1413 CTCAG DsaI 431 CCATGG C^C[GA][CT]GG 449 CCATGG EaeI 256 CGGCCG [CT]^GGCC[GA] 446 TGGCCA EcoNI 892 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1171 CCTCTTGCAGG HapII 255 CCGG C^CGG 259 CCGG HpaII 255 CCGG C^CGG 259 CCGG MaeI 775 CTAG C^TAG 887 CTAG MslI 1134 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1215 CATGTACATG MspI 255 CCGG C^CGG 259 CCGG NcoI 431 CCATGG C^CATGG 449 CCATGG NspI 1214 ACATGT [GA]CATG^[CT] 1220 ACATGT ScaI 836 AGTACT AGT^ACT 941 AGTACT SmlI 471 CTTAAG C^T[CT][GA]AG 1350 CTTGAG TaqI 927 TCGA T^CGA 933 TCGA TthHB8I 927 TCGA T^CGA 933 TCGA XcmI 40 CCAGATGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 791 CCATTATATTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BstF5I Cac8I Cfr13I EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MwoI NlaIII NlaIV RsaI Sau96I SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI