(Circular) (Six-base) MAPSORT of: pFN21AE6414.seq Check: 3287 from: 1 to: 6139 pFN21AE6414 6139 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2554 3482 1937 Size: 617 928 4594 Fragments arranged by size: 4594 928 617 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2938 4202 5586 5854 1632 Size: 1306 1264 1384 268 1917 Fragments arranged by size: 1917 1384 1306 1264 268 AflII C'TTAA_G Cuts at: 820 1017 2476 820 Size: 197 1459 4483 Fragments arranged by size: 4483 1459 197 AflIII A'CryG_T Cuts at: 1203 3219 3225 5038 6015 1203 Size: 2016 6 1813 977 1327 Fragments arranged by size: 2016 1813 1327 977 6 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 3143 1166 Size: 1977 4162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 3882 5454 3882 Size: 1572 4567 ApaLI G'TGCA_C Cuts at: 3105 4196 5352 3105 Size: 1091 1156 3892 Fragments arranged by size: 3892 1156 1091 ApoI r'AATT_y Cuts at: 1493 2142 2940 3448 3674 3826 5935 1493 Size: 649 798 508 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 649 508 226 152 AseI AT'TA_AT Cuts at: 160 1032 2909 4693 160 Size: 872 1877 1784 1606 Fragments arranged by size: 1877 1784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3464 1870 Size: 73 1521 4545 Fragments arranged by size: 4545 1521 73 AvrII C'CTAG_G Cuts at: 2779 2779 Size: 6139 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2324 2357 2324 Size: 33 6106 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 4915 618 Size: 325 217 627 1673 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3458 1391 Size: 2067 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3341 928 Size: 971 1442 3726 Fragments arranged by size: 3726 1442 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4330 4364 1903 Size: 34 50 34 2309 34 3678 Fragments arranged by size: 3678 2309 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BfrBI ATG'CAT Cuts at: 2108 2108 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 4750 136 Size: 107 122 71 631 605 901 2177 1525 Fragments arranged by size: 2177 1525 901 631 605 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2026 2999 3109 4200 5356 1790 Size: 236 973 110 1091 1156 2573 Fragments arranged by size: 2573 1156 1091 973 236 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3152 3870 4828 Size: 416 53 83 399 24 1735 718 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1753 1735 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2028 3225 4799 1239 Size: 789 1197 1574 2579 Fragments arranged by size: 2579 1574 1197 789 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3376 4264 5129 5427 3376 Size: 888 865 298 4088 Fragments arranged by size: 4088 888 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2584 4802 882 Size: 617 1085 2218 2219 Fragments arranged by size: 2219 2218 1085 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 1178 Size: 30 165 30 808 30 5076 Fragments arranged by size: 5076 808 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3165 1186 Size: 1979 4160 BseYI C'CCAG_C Cuts at: 5342 5838 5342 Size: 496 5643 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2264 3589 4350 5378 1540 Size: 275 186 263 1325 761 1028 2301 Fragments arranged by size: 2301 1325 1028 761 275 263 186 BsiHKAI G_wGCw'C Cuts at: 3109 4200 4285 5356 3109 Size: 1091 85 1071 3892 Fragments arranged by size: 3892 1091 1071 85 BsmI GAATG_Cn' Cuts at: 2108 2569 3650 3743 2108 Size: 461 1081 93 4504 Fragments arranged by size: 4504 1081 461 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2999 3109 3458 4200 4285 5356 Size: 399 236 973 110 349 742 85 1071 Cuts at: 5356 1391 Size: 2174 Fragments arranged by size: 2174 1071 973 742 399 349 236 110 85 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 2586 4028 5758 2586 Size: 1442 1730 2967 Fragments arranged by size: 2967 1730 1442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2394 3173 3494 844 Size: 1550 779 321 3489 Fragments arranged by size: 3489 1550 779 321 BsrBI CCG'CTC Cuts at: 1394 1823 4026 1394 Size: 429 2203 3507 Fragments arranged by size: 3507 2203 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3283 4634 4808 65 Size: 1183 2035 1351 174 1396 Fragments arranged by size: 2035 1396 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2263 4783 4970 1700 Size: 110 449 4 2520 187 2869 Fragments arranged by size: 2869 2520 449 187 110 4 BsrGI T'GTAC_A Cuts at: 96 3222 96 Size: 3126 3013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 3098 4199 5211 3098 Size: 1101 1012 4026 Fragments arranged by size: 4026 1101 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 6139 BstYI r'GATC_y Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134 Size: 1497 367 385 17 1452 86 12 346 Cuts at: 6134 1972 Size: 1977 Fragments arranged by size: 1977 1497 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2436 2454 513 Size: 552 1371 18 4198 Fragments arranged by size: 4198 1371 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5400 Fragments arranged by size: 5400 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3655 4449 4469 3076 Size: 579 794 20 4746 Fragments arranged by size: 4746 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DraIII CAC_nnn'GTG Cuts at: 3410 3410 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2261 2451 3586 Size: 54 1425 50 275 18 431 190 1135 Cuts at: 3586 4475 6104 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 431 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2261 3586 1537 Size: 275 449 1325 4090 Fragments arranged by size: 4090 1325 449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4069 1353 Size: 2716 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2938 3225 4202 4799 5586 5854 Size: 393 396 910 287 977 597 787 268 Cuts at: 5854 1239 Size: 1524 Fragments arranged by size: 1524 977 910 787 597 396 393 287 268 EcoNI CCTnn'n_nnAGG Cuts at: 2901 3180 2901 Size: 279 5860 EcoO109I rG'GnC_Cy Cuts at: 2222 2222 Size: 6139 EcoRI G'AATT_C Cuts at: 2940 3448 3826 2940 Size: 508 378 5253 Fragments arranged by size: 5253 508 378 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 5286 1678 Size: 3608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 4780 4812 4854 Size: 32 135 32 1256 32 1920 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 1920 1256 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3385 3483 3729 669 Size: 988 281 1447 98 246 3079 Fragments arranged by size: 3079 1447 988 281 246 98 HindIII A'AGCT_T Cuts at: 748 2533 748 Size: 1785 4354 HpaI GTT'AAC Cuts at: 3385 3729 3385 Size: 344 5795 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3141 3738 3141 Size: 597 5542 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2042 2053 3109 5253 5437 1305 Size: 737 11 1056 2144 184 2007 Fragments arranged by size: 2144 2007 1056 737 184 11 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 10 Size: 54 1425 343 621 3696 Fragments arranged by size: 3696 1425 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3224 4097 4456 4615 518 Size: 648 1977 81 873 359 159 2042 Fragments arranged by size: 2042 1977 873 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2338 3555 4232 5380 5625 1606 Size: 732 1217 677 1148 245 2120 Fragments arranged by size: 2120 1217 1148 732 677 245 NaeI GCC'GGC Cuts at: 2261 2261 Size: 6139 NcoI C'CATG_G Cuts at: 513 1065 2436 2454 513 Size: 552 1371 18 4198 Fragments arranged by size: 4198 1371 552 18 NdeI CA'TA_TG Cuts at: 387 2815 387 Size: 2428 3711 NgoMIV G'CCGG_C Cuts at: 2259 2259 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NsiI A_TGCA'T Cuts at: 2110 2110 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 3223 3229 3497 5042 1132 Size: 97 1994 6 268 1545 2229 Fragments arranged by size: 2229 1994 1545 268 97 6 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 3219 3225 5038 3219 Size: 6 1813 4320 Fragments arranged by size: 4320 1813 6 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2222 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 3491 830 Size: 2661 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2338 2338 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 2843 2948 4387 6099 2843 Size: 105 1439 1712 2883 Fragments arranged by size: 2883 1712 1439 105 SfcI C'TryA_G Cuts at: 826 1045 2167 2422 2901 3289 3487 4622 5303 Size: 219 1122 255 479 388 198 1135 681 Cuts at: 5303 5494 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1122 681 479 388 255 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2476 3355 4243 5144 5406 820 Size: 197 926 533 879 888 901 262 1553 Fragments arranged by size: 1553 926 901 888 879 533 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 2154 4063 5 Size: 47 2102 1909 2081 Fragments arranged by size: 2102 2081 1909 47 StuI AGG'CCT Cuts at: 1517 2896 1517 Size: 1379 4760 StyI C'CwwG_G Cuts at: 513 1065 2436 2454 2596 2779 2827 3049 3578 Size: 552 1371 18 142 183 48 222 529 Cuts at: 3578 513 Size: 3074 Fragments arranged by size: 3074 1371 552 529 222 183 142 48 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3092 4166 4183 4336 4521 4966 325 Size: 1255 1512 1074 17 153 185 445 1498 Fragments arranged by size: 1512 1498 1255 1074 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 2946 3222 Size: 274 80 33 51 1443 864 105 276 Cuts at: 3222 4385 6097 96 Size: 1163 1712 138 Fragments arranged by size: 1712 1443 1163 864 276 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2052 2803 2052 Size: 751 5388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI