Restriction map for
pFN21AE6414
(Linear) (Six-base) MAPSORT of: KIEE6414.seq Check: 4464 from: 1 to: 1434
KIEE6414.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 549 1434
Size: 549 885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1434
Size: 933 501
AflII C'TTAA_G
Cuts at: 0 471 1434
Size: 471 963
AflIII A'CryG_T
Cuts at: 0 1214 1220 1434
Size: 1214 6 214
Fragments arranged by size:
1214 214 6
AleI CACnn'nnGTG
Cuts at: 0 1138 1434
Size: 1138 296
ApaLI G'TGCA_C
Cuts at: 0 1100 1434
Size: 1100 334
ApoI r'AATT_y
Cuts at: 0 137 935 1434
Size: 137 798 499
Fragments arranged by size:
798 499 137
AseI AT'TA_AT
Cuts at: 0 904 1434
Size: 904 530
AvrII C'CTAG_G
Cuts at: 0 774 1434
Size: 774 660
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 319 352 1434
Size: 319 33 1082
Fragments arranged by size:
1082 319 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1336 1434
Size: 1336 98
BfrBI ATG'CAT
Cuts at: 0 103 1434
Size: 103 1331
BglI GCCn_nnn'nGGC
Cuts at: 0 568 1434
Size: 568 866
Bme1580I G_kGCm'C
Cuts at: 0 21 994 1104 1434
Size: 21 973 110 330
Fragments arranged by size:
973 330 110 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1147 1434
Size: 1147 287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 1220 1434
Size: 23 1197 214
Fragments arranged by size:
1197 214 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1371 1434
Size: 1371 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 579 1434
Size: 579 855
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 206 236 1434
Size: 206 30 1198
Fragments arranged by size:
1198 206 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1160 1434
Size: 1160 274
BsiEI CG_ry'CG
Cuts at: 0 259 1434
Size: 259 1175
BsiHKAI G_wGCw'C
Cuts at: 0 1104 1434
Size: 1104 330
BsmI GAATG_Cn'
Cuts at: 0 103 564 1434
Size: 103 461 870
Fragments arranged by size:
870 461 103
Bsp1286I G_dGCh'C
Cuts at: 0 21 994 1104 1434
Size: 21 973 110 330
Fragments arranged by size:
973 330 110 21
BspHI T'CATG_A
Cuts at: 0 581 1434
Size: 581 853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 389 1168 1434
Size: 389 779 266
Fragments arranged by size:
779 389 266
BsrDI GCAATG_nn'
Cuts at: 0 1278 1434
Size: 1278 156
BsrFI r'CCGG_y
Cuts at: 0 254 258 1434
Size: 254 4 1176
Fragments arranged by size:
1176 254 4
BsrGI T'GTAC_A
Cuts at: 0 1217 1434
Size: 1217 217
BssSI C'ACGA_G
Cuts at: 0 1093 1434
Size: 1093 341
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1434
Size: 195 1239
BtgI C'CryG_G
Cuts at: 0 431 449 1434
Size: 431 18 985
Fragments arranged by size:
985 431 18
BtsI GCAGTG_nn'
Cuts at: 0 1071 1434
Size: 1071 363
DraIII CAC_nnn'GTG
Cuts at: 0 1405 1434
Size: 1405 29
EaeI y'GGCC_r
Cuts at: 0 256 446 1434
Size: 256 190 988
Fragments arranged by size:
988 256 190
EagI C'GGCC_G
Cuts at: 0 256 1434
Size: 256 1178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 933 1220 1434
Size: 23 910 287 214
Fragments arranged by size:
910 287 214 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 896 1175 1434
Size: 896 279 259
Fragments arranged by size:
896 279 259
EcoO109I rG'GnC_Cy
Cuts at: 0 217 1434
Size: 217 1217
EcoRI G'AATT_C
Cuts at: 0 935 1434
Size: 935 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1434
Size: 823 32 579
Fragments arranged by size:
823 579 32
HincII GTy'rAC
Cuts at: 0 1380 1434
Size: 1380 54
HindIII A'AGCT_T
Cuts at: 0 528 1434
Size: 528 906
HpaI GTT'AAC
Cuts at: 0 1380 1434
Size: 1380 54
MfeI C'AATT_G
Cuts at: 0 1136 1434
Size: 1136 298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 48 1104 1434
Size: 37 11 1056 330
Fragments arranged by size:
1056 330 37 11
MscI TGG'CCA
Cuts at: 0 448 1434
Size: 448 986
MslI CAynn'nnrTG
Cuts at: 0 1138 1219 1434
Size: 1138 81 215
Fragments arranged by size:
1138 215 81
MspA1I CmG'CkG
Cuts at: 0 333 1434
Size: 333 1101
NaeI GCC'GGC
Cuts at: 0 256 1434
Size: 256 1178
NcoI C'CATG_G
Cuts at: 0 431 449 1434
Size: 431 18 985
Fragments arranged by size:
985 431 18
NdeI CA'TA_TG
Cuts at: 0 810 1434
Size: 810 624
NgoMIV G'CCGG_C
Cuts at: 0 254 1434
Size: 254 1180
NsiI A_TGCA'T
Cuts at: 0 105 1434
Size: 105 1329
NspI r_CATG'y
Cuts at: 0 1218 1224 1434
Size: 1218 6 210
Fragments arranged by size:
1218 210 6
PciI A'CATG_T
Cuts at: 0 1214 1220 1434
Size: 1214 6 214
Fragments arranged by size:
1214 214 6
PpuMI rG'GwC_Cy
Cuts at: 0 217 1434
Size: 217 1217
PvuII CAG'CTG
Cuts at: 0 333 1434
Size: 333 1101
ScaI AGT'ACT
Cuts at: 0 838 943 1434
Size: 838 105 491
Fragments arranged by size:
838 491 105
SfcI C'TryA_G
Cuts at: 0 162 417 896 1284 1434
Size: 162 255 479 388 150
Fragments arranged by size:
479 388 255 162 150
SmlI C'TyrA_G
Cuts at: 0 471 1350 1434
Size: 471 879 84
Fragments arranged by size:
879 471 84
SspI AAT'ATT
Cuts at: 0 149 1434
Size: 149 1285
StuI AGG'CCT
Cuts at: 0 891 1434
Size: 891 543
StyI C'CwwG_G
Cuts at: 0 431 449 591 774 822 1044 1434
Size: 431 18 142 183 48 222 390
Fragments arranged by size:
431 390 222 183 142 48 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1087 1434
Size: 1087 347
TatI w'GTAC_w
Cuts at: 0 836 941 1217 1434
Size: 836 105 276 217
Fragments arranged by size:
836 276 217 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 47 798 1434
Size: 47 751 636
Fragments arranged by size:
751 636 47
Enzymes that do cut:
AccI AcuI AflII AflIII AleI ApaLI ApoI AseI
AvrII BaeI BbsI BfrBI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI BtgI
BtsI DraIII EaeI EagI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI
PpuMI PvuII ScaI SfcI SmlI SspI StuI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AlwNI ApaI AscI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiWI BsmBI BspEI BsrBI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MluI NarI NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI