(Circular) (Six-base) MAPSORT of: pFN21AB3060.seq Check: 111 from: 1 to: 6583 pFN21AB3060 6583 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 3771 4710 5083 1563 Size: 2208 939 373 3063 Fragments arranged by size: 3063 2208 939 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2932 3700 4646 6030 6298 1632 Size: 1300 768 946 1384 268 1917 Fragments arranged by size: 1917 1384 1300 946 768 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2348 2390 1184 Size: 1164 42 5377 Fragments arranged by size: 5377 1164 42 AlwNI CAG_nnn'CTG Cuts at: 2023 2476 2758 3254 4326 5898 2023 Size: 453 282 496 1072 1572 2708 Fragments arranged by size: 2708 1572 1072 496 453 282 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3892 4118 4270 6379 1493 Size: 2399 226 152 2109 1697 Fragments arranged by size: 2399 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 3180 3908 1870 Size: 73 1237 728 4545 Fragments arranged by size: 4545 1237 728 73 BamHI G'GATC_C Cuts at: 3087 3913 4280 3087 Size: 826 367 5390 Fragments arranged by size: 5390 826 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2861 2933 3904 5359 Size: 325 217 627 398 676 72 971 1455 Cuts at: 5359 618 Size: 1842 Fragments arranged by size: 1842 1455 971 676 627 398 325 217 72 BanII G_rGCy'C Cuts at: 1391 3357 3452 3902 1391 Size: 1966 95 450 4072 Fragments arranged by size: 4072 1966 450 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3062 3250 3389 3706 928 Size: 971 1163 188 139 317 3805 Fragments arranged by size: 3805 1163 971 317 188 139 BbvCI CC'TCA_GC Cuts at: 2487 2487 Size: 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4774 4808 1903 Size: 34 2837 34 3678 Fragments arranged by size: 3678 2837 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2916 4475 5685 2916 Size: 1559 1210 3814 Fragments arranged by size: 3814 1559 1210 BclI T'GATC_A Cuts at: 1548 3874 1548 Size: 2326 4257 BfrBI ATG'CAT Cuts at: 3531 3531 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5194 136 Size: 107 122 71 631 605 3522 1525 Fragments arranged by size: 3522 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 2652 3729 4644 5800 1790 Size: 862 1077 915 1156 2573 Fragments arranged by size: 2573 1156 1077 915 862 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 3119 3353 Size: 416 53 83 399 24 738 964 234 Cuts at: 3353 3644 4314 5272 442 Size: 291 670 958 1753 Fragments arranged by size: 1753 964 958 738 670 416 399 291 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3052 3839 3052 Size: 787 5796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5243 1239 Size: 4004 2579 Bpu10I CC'TnA_GC Cuts at: 2487 2813 3491 2487 Size: 326 678 5579 Fragments arranged by size: 5579 678 326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3023 5246 882 Size: 617 1524 2223 2219 Fragments arranged by size: 2223 2219 1524 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 3366 4374 493 Size: 711 918 1244 1008 2702 Fragments arranged by size: 2702 1244 1008 918 711 BsaBI GATnn'nnATC Cuts at: 2450 6577 2450 Size: 4127 2456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4390 4772 5407 275 Size: 53 83 186 936 2857 382 635 1451 Fragments arranged by size: 2857 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 2220 5016 5414 5688 5835 6246 2034 Size: 186 2796 398 274 147 411 2371 Fragments arranged by size: 2796 2371 411 398 274 186 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3756 3786 1178 Size: 30 165 30 2353 30 3975 Fragments arranged by size: 3975 2353 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3263 1186 Size: 2077 4506 BseYI C'CCAG_C Cuts at: 2096 3204 3568 3844 5786 6282 2096 Size: 1108 364 276 1942 496 2397 Fragments arranged by size: 2397 1942 1108 496 364 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3605 3622 1163 Size: 573 1869 17 4124 Fragments arranged by size: 4124 1869 573 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3339 3452 4644 4729 5800 3339 Size: 113 1192 85 1071 4122 Fragments arranged by size: 4122 1192 1071 113 85 BsmI GAATG_Cn' Cuts at: 4094 4187 4094 Size: 93 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2652 3339 3357 3452 3729 3902 4644 Size: 399 862 687 18 95 277 173 742 Cuts at: 4644 4729 5800 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 862 742 687 399 277 173 95 85 18 BspEI T'CCGG_A Cuts at: 2034 6246 2034 Size: 4212 2371 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 3041 3938 844 Size: 1478 719 897 3489 Fragments arranged by size: 3489 1478 897 719 BsrBI CCG'CTC Cuts at: 1394 1823 2065 4470 1394 Size: 429 242 2405 3507 Fragments arranged by size: 3507 2405 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 5078 5252 65 Size: 1183 1077 2753 174 1396 Fragments arranged by size: 2753 1396 1183 1077 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2188 5227 5414 1700 Size: 110 378 3039 187 2869 Fragments arranged by size: 3039 2869 378 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 2110 2501 3536 4643 5655 2110 Size: 391 1035 1107 1012 3038 Fragments arranged by size: 3038 1107 1035 1012 391 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 794 Size: 1229 5354 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2440 2545 2233 Size: 207 105 6271 Fragments arranged by size: 6271 207 105 BstYI r'GATC_y Cuts at: 1972 3087 3913 4280 4665 4682 6134 6220 6232 Size: 1115 826 367 385 17 1452 86 12 Cuts at: 6232 6578 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 826 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 2439 3506 513 Size: 552 939 294 141 1067 3590 Fragments arranged by size: 3590 1067 939 552 294 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5844 Fragments arranged by size: 5844 683 56 BtsI GCAGTG_nn' Cuts at: 2166 3681 3727 4099 4893 4913 2166 Size: 1515 46 372 794 20 3836 Fragments arranged by size: 3836 1515 794 372 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3025 3887 3025 Size: 862 5721 DraIII CAC_nnn'GTG Cuts at: 3660 3660 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 3798 4030 Size: 54 1425 50 275 18 519 1449 232 Cuts at: 4030 4919 6548 8 Size: 889 1629 43 Fragments arranged by size: 1629 1449 1425 889 519 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 2589 2951 3077 3281 3830 4513 1353 Size: 1167 69 362 126 204 549 683 3423 Fragments arranged by size: 3423 1167 683 549 362 204 126 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 5170 5544 5690 387 Size: 1064 138 666 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 666 374 146 138 EcoICRI GAG'CTC Cuts at: 3450 3450 Size: 6583 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2932 3700 4646 5243 6030 6298 1239 Size: 393 1300 768 946 597 787 268 1524 Fragments arranged by size: 1524 1300 946 787 768 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 2020 2775 2020 Size: 755 5828 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3319 3319 Size: 6583 FspI TGC'GCA Cuts at: 2336 5089 2336 Size: 2753 3830 HaeII r_GCGC'y Cuts at: 1678 5730 1678 Size: 4052 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3022 3054 5224 5256 5298 Size: 32 135 32 1450 32 2170 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2626 2170 1450 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3769 3927 4173 669 Size: 988 281 1831 158 246 3079 Fragments arranged by size: 3079 1831 988 281 246 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 2445 2541 2625 5697 5881 1305 Size: 828 312 96 84 3072 184 2007 Fragments arranged by size: 3072 2007 828 312 184 96 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 3800 10 Size: 54 1425 343 1968 2793 Fragments arranged by size: 2793 1968 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2438 2444 2621 2975 3158 3536 Size: 648 1119 153 6 177 354 183 378 Cuts at: 3536 4541 4900 5059 518 Size: 1005 359 159 2042 Fragments arranged by size: 2042 1119 1005 648 378 359 354 183 177 159 153 6 MspA1I CmG'CkG Cuts at: 1606 2985 3999 4676 5824 6069 1606 Size: 1379 1014 677 1148 245 2120 Fragments arranged by size: 2120 1379 1148 1014 677 245 NaeI GCC'GGC Cuts at: 2190 2190 Size: 6583 NcoI C'CATG_G Cuts at: 513 1065 2004 2439 513 Size: 552 939 435 4657 Fragments arranged by size: 4657 939 552 435 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NgoMIV G'CCGG_C Cuts at: 2188 2188 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NsiI A_TGCA'T Cuts at: 3533 3533 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2371 3531 3941 5486 1132 Size: 97 1142 1160 410 1545 2229 Fragments arranged by size: 2229 1545 1160 1142 410 97 PasI CC'CwG_GG Cuts at: 1348 3393 1348 Size: 2045 4538 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 3393 1108 Size: 2285 4298 PfoI T'CCnGG_A Cuts at: 2883 2883 Size: 6583 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PmlI CAC'GTG Cuts at: 3366 3366 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2775 2775 Size: 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2753 2785 2753 Size: 32 6551 PstI C_TGCA'G Cuts at: 830 2019 2179 2472 3935 830 Size: 1189 160 293 1463 3478 Fragments arranged by size: 3478 1463 1189 293 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2985 2985 Size: 6583 SacI G_AGCT'C Cuts at: 3452 3452 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 2246 4831 6543 2246 Size: 2585 1712 2286 Fragments arranged by size: 2585 2286 1712 SexAI A'CCwGG_T Cuts at: 2936 2936 Size: 6583 SfcI C'TryA_G Cuts at: 826 1045 2015 2175 2468 3931 5066 5747 5938 Size: 219 970 160 293 1463 1135 681 191 Cuts at: 5938 826 Size: 1471 Fragments arranged by size: 1471 1463 1135 970 681 293 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3182 3910 1872 Size: 1310 728 4545 Fragments arranged by size: 4545 1310 728 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 2371 3531 3941 1132 Size: 1239 1160 410 3774 Fragments arranged by size: 3774 1239 1160 410 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 2108 1517 Size: 591 5992 StyI C'CwwG_G Cuts at: 513 1065 2004 2439 3262 4022 513 Size: 552 939 435 823 760 3074 Fragments arranged by size: 3074 939 823 760 552 435 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 2721 3365 4610 4627 4780 4965 Size: 1255 557 584 644 1245 17 153 185 Cuts at: 4965 5410 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1245 644 584 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 2244 2769 Size: 274 80 33 51 1443 165 102 525 Cuts at: 2769 4829 6541 96 Size: 2060 1712 138 Fragments arranged by size: 2060 1712 1443 525 274 165 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6543 6575 6543 Size: 32 6551 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2437 2266 Size: 171 6412 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3180 3908 1870 Size: 1310 728 4545 Fragments arranged by size: 4545 1310 728 XmnI GAAnn'nnTTC Cuts at: 3374 4712 3374 Size: 1338 5245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI