################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI StyI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### ApaI 1664 GGGCCC GGGCC^C Asp700I 1418 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 560 GGACC G^G[AT]CC BanI 1714 GGTGCC G^G[CT][GA]CC BclI 1164 TGATCA T^GATCA BglII 750 AGATCT A^GATCT BsaAI 434 TACGTG [CT]AC^GT[GA] BsaBI 1531 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1257 TCCGGT [AT]^CCGG[AT] BsmFI 887 GTCCC GGGAC(10/14) Bsp1407I 528 TGTACA T^GTACA BsrBI 1743 GAGCGG CCGCTC(-3/-3) BsrGI 528 TGTACA T^GTACA BstAPI 1615 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 472 GGTTACC G^GT[ACGT]ACC BstPI 472 GGTTACC G^GT[ACGT]ACC BstXI 973 CCAACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1830 TTTAAA TTT^AAA DsaI 1595 CCACGG C^C[GA][CT]GG EarI 2307 GAAGAG CTCTTC(1/4) EcoO65I 472 GGTTACC G^GT[ACGT]ACC FbaI 1164 TGATCA T^GATCA HapII 1258 CCGG C^CGG HpaII 1258 CCGG C^CGG Ksp632I 2307 GAAGAG CTCTTC(1/4) MamI 1531 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1258 CCGG C^CGG NspI 1547 GCATGC [GA]CATG^[CT] PflFI 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1605 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SbfI 1726 CCTGCAGG CCTGCA^GG SphI 1547 GCATGC GCATG^C Sse8387I 1726 CCTGCAGG CCTGCA^GG SspBI 528 TGTACA T^GTACA StuI 2024 AGGCCT AGG^CCT Tth111I 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1605 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1418 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 413 GCGG CCGC(-3/-1) 1745 GCGG AcsI 845 AAATTT [GA]^AATT[CT] 2332 AAATTT AocI 1427 CCTCAGG CC^T[ACGT]AGG 1757 CCTCAGG ApoI 845 AAATTT [GA]^AATT[CT] 2332 AAATTT BalI 899 TGGCCA TGG^CCA 982 TGGCCA BanII 1650 GGGCTC G[GA]GC[CT]^C 1664 GGGCCC BbsI 568 GAAGAC GAAGAC(2/6) 1353 GTCTTC BpuAI 568 GAAGAC GAAGAC(2/6) 1353 GTCTTC BsaI 63 GAGACC GGTCTC(1/5) 298 GAGACC BsgI 648 CTGCAC GTGCAG(16/14) 32 GTGCAG BsrDI 409 CATTGC GCAATG(2/0) 1792 GCAATG Bsu36I 1427 CCTCAGG CC^T[ACGT]AGG 1757 CCTCAGG DraII 1663 GGGGCCC [GA]G^G[ACGT]CC[CT] 1852 AGGCCCT Eco81I 1427 CCTCAGG CC^T[ACGT]AGG 1757 CCTCAGG EcoO109I 1663 GGGGCCC [GA]G^G[ACGT]CC[CT] 1852 AGGCCCT HindIII 8 AAGCTT A^AGCTT 196 AAGCTT HphI 644 TCACC GGTGA(8/7) 1570 TCACC MaeII 435 ACGT A^CGT 498 ACGT MluNI 899 TGGCCA TGG^CCA 982 TGGCCA MscI 899 TGGCCA TGG^CCA 982 TGGCCA PstI 1727 CTGCAG CTGCA^G 1903 CTGCAG SspI 338 AATATT AAT^ATT 400 AATATT XcmI 1601 CCACCCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1869 CCAGCTTCCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AfaI AluI AlwI BbvCI BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII