(Circular) (Six-base) MAPSORT of: pFN21AB6273.seq Check: 8373 from: 1 to: 7042 pFN21AB6273 7042 bp With 182 enzymes: SgfI * February 5, 2010 17:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2660 3586 3809 2660 Size: 926 223 5893 Fragments arranged by size: 5893 926 223 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4852 278 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4385 1937 Size: 2448 4594 Acc65I G'GTAC_C Cuts at: 4363 4363 Size: 7042 AclI AA'CG_TT Cuts at: 1563 5169 5542 1563 Size: 3606 373 3063 Fragments arranged by size: 3606 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2937 5105 6489 6757 1632 Size: 549 756 2168 1384 268 1917 Fragments arranged by size: 2168 1917 1384 756 549 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6845 AflIII A'CryG_T Cuts at: 1203 5941 6918 1203 Size: 4738 977 1327 Fragments arranged by size: 4738 1327 977 AgeI A'CCGG_T Cuts at: 5873 5873 Size: 7042 AhdI GACnn_n'nnGTC Cuts at: 5771 5771 Size: 7042 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2668 1184 Size: 1484 5558 AlwNI CAG_nnn'CTG Cuts at: 4785 6357 4785 Size: 1572 5470 ApaI G_GGCC'C Cuts at: 3673 3673 Size: 7042 ApaLI G'TGCA_C Cuts at: 5099 6255 5099 Size: 1156 5886 ApoI r'AATT_y Cuts at: 1493 2850 4337 4351 4577 4729 6838 1493 Size: 1357 1487 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 1357 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 5596 160 Size: 872 4564 1606 Fragments arranged by size: 4564 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 AvaI C'yCGr_G Cuts at: 1870 1943 4367 1870 Size: 73 2424 4545 Fragments arranged by size: 4545 2424 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4193 4226 4193 Size: 33 7009 BamHI G'GATC_C Cuts at: 4372 4739 4372 Size: 367 6675 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3719 4363 5818 618 Size: 325 217 627 1932 644 1455 1842 Fragments arranged by size: 1932 1842 1455 644 627 325 217 BanII G_rGCy'C Cuts at: 1391 3659 3673 4361 1391 Size: 2268 14 688 4072 Fragments arranged by size: 4072 2268 688 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2580 3351 928 Size: 971 681 771 4619 Fragments arranged by size: 4619 971 771 681 BbvCI CC'TCA_GC Cuts at: 2781 2786 3954 4152 2781 Size: 5 1168 198 5671 Fragments arranged by size: 5671 1168 198 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5233 5267 1903 Size: 34 50 34 3212 34 3678 Fragments arranged by size: 3678 3212 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4934 6144 4934 Size: 1210 5832 BclI T'GATC_A Cuts at: 1548 3169 1548 Size: 1621 5421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5653 136 Size: 107 122 71 631 605 3981 1525 Fragments arranged by size: 3981 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2755 7037 2755 Size: 4282 2760 BlpI GC'TnA_GC Cuts at: 3292 4041 4263 4459 3292 Size: 749 222 196 5875 Fragments arranged by size: 5875 749 222 196 Bme1580I G_kGCm'C Cuts at: 1790 2062 3673 5103 6259 1790 Size: 272 1611 1430 1156 2573 Fragments arranged by size: 2573 1611 1430 1156 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 3928 4773 Size: 416 53 83 399 24 2369 142 845 Cuts at: 4773 5731 442 Size: 958 1753 Fragments arranged by size: 2369 1753 958 845 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3268 3300 3546 3857 4089 5702 1239 Size: 2029 32 246 311 232 1613 2579 Fragments arranged by size: 2579 2029 1613 311 246 232 32 Bpu10I CC'TnA_GC Cuts at: 2276 2781 2786 3418 3746 3954 4137 4152 2276 Size: 505 5 632 328 208 183 15 5166 Fragments arranged by size: 5166 632 505 328 208 183 15 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 3397 3798 5167 6032 6330 2609 Size: 788 401 1369 865 298 3321 Fragments arranged by size: 3321 1369 865 788 401 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2062 2297 5705 882 Size: 617 563 235 3408 2219 Fragments arranged by size: 3408 2219 617 563 235 BsaAI yAC'GTr Cuts at: 493 1204 2441 4833 493 Size: 711 1237 2392 2702 Fragments arranged by size: 2702 2392 1237 711 BsaBI GATnn'nnATC Cuts at: 3540 7036 3540 Size: 3496 3546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4849 5231 5866 275 Size: 53 83 186 936 3316 382 635 1451 Fragments arranged by size: 3316 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3262 5475 5873 6147 6294 6705 3262 Size: 2213 398 274 147 411 3599 Fragments arranged by size: 3599 2213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2881 2911 3475 3505 1178 Size: 30 165 30 1478 30 564 30 4715 Fragments arranged by size: 4715 1478 564 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 2769 3477 3480 4162 1186 Size: 1580 3 708 3 682 4066 Fragments arranged by size: 4066 1580 708 682 3 3 BseYI C'CCAG_C Cuts at: 3703 6245 6741 3703 Size: 2542 496 4004 Fragments arranged by size: 4004 2542 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 2638 1163 Size: 573 322 580 5567 Fragments arranged by size: 5567 580 573 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4492 5253 6281 1540 Size: 275 186 2491 761 1028 2301 Fragments arranged by size: 2491 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5103 5188 6259 5103 Size: 85 1071 5886 Fragments arranged by size: 5886 1071 85 BsmI GAATG_Cn' Cuts at: 4553 4646 4553 Size: 93 6949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 3659 3673 4361 5103 5188 6259 Size: 399 272 1597 14 688 742 85 1071 Cuts at: 6259 1391 Size: 2174 Fragments arranged by size: 2174 1597 1071 742 688 399 272 85 14 BspEI T'CCGG_A Cuts at: 6705 6705 Size: 7042 BspHI T'CATG_A Cuts at: 4931 6661 4931 Size: 1730 5312 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2660 2819 3586 3809 4397 844 Size: 1816 159 767 223 588 3489 Fragments arranged by size: 3489 1816 767 588 223 159 BsrBI CCG'CTC Cuts at: 1394 1823 3750 4929 1394 Size: 429 1927 1179 3507 Fragments arranged by size: 3507 1927 1179 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2413 3804 5537 5711 65 Size: 1183 1165 1391 1733 174 1396 Fragments arranged by size: 1733 1396 1391 1183 1165 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5686 5873 1700 Size: 110 3876 187 2869 Fragments arranged by size: 3876 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2533 96 Size: 2437 4605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7042 BssSI C'ACGA_G Cuts at: 5102 6114 5102 Size: 1012 6030 BstAPI GCAn_nnn'nTGC Cuts at: 794 3626 794 Size: 2832 4210 BstBI TT'CG_AA Cuts at: 5846 5846 Size: 7042 BstEII G'GTnAC_C Cuts at: 1184 2477 1184 Size: 1293 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2985 2985 Size: 7042 BstYI r'GATC_y Cuts at: 1972 2732 2755 3082 3535 4372 4739 5124 5141 Size: 760 23 327 453 837 367 385 17 Cuts at: 5141 6593 6679 6691 7037 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 837 760 453 385 367 346 327 86 23 17 12 Bsu36I CC'TnA_GG Cuts at: 3433 3763 3433 Size: 330 6712 BtgI C'CryG_G Cuts at: 513 1065 3600 513 Size: 552 2535 3955 Fragments arranged by size: 3955 2535 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6303 Fragments arranged by size: 6303 683 56 BtsI GCAGTG_nn' Cuts at: 2655 3785 4558 5352 5372 2655 Size: 1130 773 794 20 4325 Fragments arranged by size: 4325 1130 794 773 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7042 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7007 DraI TTT'AAA Cuts at: 3837 4346 3837 Size: 509 6533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6049 809 Size: 507 4733 1802 Fragments arranged by size: 4733 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2904 2987 3603 Size: 54 1425 50 275 18 1074 83 616 Cuts at: 3603 4489 5378 7007 8 Size: 886 889 1629 43 Fragments arranged by size: 1629 1425 1074 889 886 616 275 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4489 1537 Size: 275 2677 4090 Fragments arranged by size: 4090 2677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4307 4972 1353 Size: 2954 665 3423 Fragments arranged by size: 3423 2954 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5629 6003 6149 387 Size: 1064 138 4040 374 146 1280 Fragments arranged by size: 4040 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2937 3268 3300 3546 3857 4089 Size: 393 549 756 331 32 246 311 232 Cuts at: 4089 5105 5702 6489 6757 1239 Size: 1016 597 787 268 1524 Fragments arranged by size: 1524 1016 787 756 597 549 393 331 311 268 246 232 32 EcoO109I rG'GnC_Cy Cuts at: 3669 3858 3669 Size: 189 6853 EcoRI G'AATT_C Cuts at: 4351 4729 4351 Size: 378 6664 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2597 2597 Size: 7042 FspI TGC'GCA Cuts at: 5548 5548 Size: 7042 HaeII r_GCGC'y Cuts at: 1678 6189 1678 Size: 4511 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2680 2712 4152 Size: 32 135 32 635 32 441 32 1440 Cuts at: 4152 4184 5683 5715 5757 5789 1373 Size: 32 1499 32 42 32 2626 Fragments arranged by size: 2626 1499 1440 635 441 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4386 4632 669 Size: 988 281 2448 246 3079 Fragments arranged by size: 3079 2448 988 281 246 HindIII A'AGCT_T Cuts at: 748 2013 2201 748 Size: 1265 188 5589 Fragments arranged by size: 5589 1265 188 HpaI GTT'AAC Cuts at: 4632 4632 Size: 7042 KpnI G_GTAC'C Cuts at: 4367 4367 Size: 7042 MfeI C'AATT_G Cuts at: 4641 4641 Size: 7042 MluI A'CGCG_T Cuts at: 6918 6918 Size: 7042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2531 3506 6156 6340 1305 Size: 1226 975 2650 184 2007 Fragments arranged by size: 2650 2007 1226 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 2989 10 Size: 54 1425 343 1074 83 4063 Fragments arranged by size: 4063 1425 1074 343 83 54 MslI CAynn'nnrTG Cuts at: 518 1166 5000 5359 5518 518 Size: 648 3834 359 159 2042 Fragments arranged by size: 3834 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2615 2785 3566 4100 4458 5135 6283 6528 Size: 1009 170 781 534 358 677 1148 245 Cuts at: 6528 1606 Size: 2120 Fragments arranged by size: 2120 1148 1009 781 677 534 358 245 170 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6490 NdeI CA'TA_TG Cuts at: 387 387 Size: 7042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7042 NotI GC'GGCC_GC Cuts at: 4489 4489 Size: 7042 NspI r_CATG'y Cuts at: 1132 1229 3556 4400 5945 1132 Size: 97 2327 844 1545 2229 Fragments arranged by size: 2327 2229 1545 844 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7042 PciI A'CATG_T Cuts at: 5941 5941 Size: 7042 PflMI CCAn_nnn'nTGG Cuts at: 1108 3616 1108 Size: 2508 4534 PmeI GTTT'AAAC Cuts at: 4346 4346 Size: 7042 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5111 5143 6624 6656 5111 Size: 32 1481 32 5497 Fragments arranged by size: 5497 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7042 PsiI TTA'TAA Cuts at: 4612 4612 Size: 7042 PspOMI G'GGCC_C Cuts at: 3669 3669 Size: 7042 PstI C_TGCA'G Cuts at: 830 3736 3912 4394 830 Size: 2906 176 482 3478 Fragments arranged by size: 3478 2906 482 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7042 PvuII CAG'CTG Cuts at: 2615 2785 3566 4100 2615 Size: 170 781 534 5557 Fragments arranged by size: 5557 781 534 170 SalI G'TCGA_C Cuts at: 1936 4384 1936 Size: 2448 4594 SbfI CC_TGCA'GG Cuts at: 3736 4394 3736 Size: 658 6384 ScaI AGT'ACT Cuts at: 5290 7002 5290 Size: 1712 5330 SfcI C'TryA_G Cuts at: 826 1045 2446 2610 2640 3732 3908 4390 5525 Size: 219 1401 164 30 1092 176 482 1135 Cuts at: 5525 6206 6397 826 Size: 681 191 1471 Fragments arranged by size: 1471 1401 1135 1092 681 482 219 191 176 164 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7042 SmaI CCC'GGG Cuts at: 1872 4369 1872 Size: 2497 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 3412 3813 5146 6047 6309 Size: 197 926 645 824 401 1333 901 262 Cuts at: 6309 820 Size: 1553 Fragments arranged by size: 1553 1333 926 901 824 645 401 262 197 SnaBI TAC'GTA Cuts at: 493 4833 493 Size: 4340 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7042 SphI G_CATG'C Cuts at: 1132 3556 4400 1132 Size: 2424 844 3774 Fragments arranged by size: 3774 2424 844 SspI AAT'ATT Cuts at: 5 52 2345 2407 4966 5 Size: 47 2293 62 2559 2081 Fragments arranged by size: 2559 2293 2081 62 47 StuI AGG'CCT Cuts at: 1517 4031 1517 Size: 2514 4528 StyI C'CwwG_G Cuts at: 513 1065 4481 513 Size: 552 3416 3074 Fragments arranged by size: 3416 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3218 3623 5069 5086 5239 5424 5869 Size: 1255 1638 405 1446 17 153 185 445 Cuts at: 5869 325 Size: 1498 Fragments arranged by size: 1638 1498 1446 1255 445 405 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 5288 7000 Size: 274 80 33 51 1443 556 2755 1712 Cuts at: 7000 96 Size: 138 Fragments arranged by size: 2755 1712 1443 556 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7002 7034 7002 Size: 32 7010 Tth111I GACn'n_nGTC Cuts at: 1316 2221 1316 Size: 905 6137 XbaI T'CTAG_A Cuts at: 4378 4378 Size: 7042 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3613 3881 3613 Size: 268 6774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7042 XmaI C'CCGG_G Cuts at: 1870 4367 1870 Size: 2497 4545 XmnI GAAnn'nnTTC Cuts at: 3427 5171 3427 Size: 1744 5298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4850 276 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI