################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanII BbeI BbvCI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1066 GACGTC GACGT^C AccI 2116 GTCGAC GT^[AC][GT]AC AcsI 68 AAATTT [GA]^AATT[CT] AcyI 1066 GACGTC G[GA]^CG[CT]C AflII 1114 CTTAAG C^TTAAG ApoI 68 AAATTT [GA]^AATT[CT] AseI 1648 ATTAAT AT^TAAT AsnI 1648 ATTAAT AT^TAAT BamHI 957 GGATCC G^GATCC BbrPI 2017 CACGTG CAC^GTG BbsI 94 GAAGAC GAAGAC(2/6) BciVI 1490 GTATCC GTATCC(6/5) BfaI 1328 CTAG C^TAG BfrI 1114 CTTAAG C^TTAAG BpuAI 94 GAAGAC GAAGAC(2/6) BsaHI 1066 GACGTC G[GA]^CG[CT]C BsmBI 1534 GAGACG CGTCTC(1/5) Bsp1407I 303 TGTACA T^GTACA BspHI 464 TCATGA T^CATGA BspLU11I 2084 ACATGT A^CATGT BsrBI 1126 CCGCTC CCGCTC(-3/-3) BsrGI 303 TGTACA T^GTACA BstEII 1272 GGTGACC G^GT[ACGT]ACC BstPI 1272 GGTGACC G^GT[ACGT]ACC BstXI 1037 CCAACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 957 GGATCC [GA]^GATC[CT] Cac8I 2032 GCGGGC GC[ACGT]^[ACGT]GC CfoI 2101 GCGC GCG^C DrdI 1924 GACCGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2037 CCACGG C^C[GA][CT]GG EarI 964 GAAGAG CTCTTC(1/4) EcoNI 795 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1272 GGTGACC G^GT[ACGT]ACC EcoT14I 2027 CCAAGG C^C[AT][AT]GG HhaI 2101 GCGC GCG^C Hin1I 1066 GACGTC G[GA]^CG[CT]C HinP1I 2101 GCGC G^CGC HincII 2116 GTCGAC GT[CT]^[GA]AC HindII 2116 GTCGAC GT[CT]^[GA]AC Ksp632I 964 GAAGAG CTCTTC(1/4) MaeI 1328 CTAG C^TAG MflI 957 GGATCC [GA]^GATC[CT] PflMI 636 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2017 CACGTG CAC^GTG PmlI 2017 CACGTG CAC^GTG PshBI 1648 ATTAAT AT^TAAT PstI 1081 CTGCAG CTGCA^G PvuII 519 CAGCTG CAG^CTG RcaI 464 TCATGA T^CATGA SalI 2116 GTCGAC G^TCGAC ScaI 981 AGTACT AGT^ACT SspBI 303 TGTACA T^GTACA SspI 410 AATATT AAT^ATT StyI 2027 CCAAGG C^C[AT][AT]GG Van91I 636 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 957 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 23 ACGTGT A^C[GA][CT]GT 2084 ACATGT AspHI 1106 GTGCTC G[AT]GC[AT]^C 1800 GTGCTC BalI 1502 TGGCCA TGG^CCA 1559 TGGCCA BanI 478 GGTGCC G^G[CT][GA]CC 2105 GGTGCC BclI 938 TGATCA T^GATCA 1610 TGATCA BmyI 1106 GTGCTC G[AGT]GC[ACT]^C 1800 GTGCTC BpmI 1208 CTCCAG CTGGAG(16/14) 1249 CTGGAG BsaAI 874 TACGTG [CT]AC^GT[GA] 2017 CACGTG BsaI 37 GAGACC GGTCTC(1/5) 1698 GAGACC BsiHKAI 1106 GTGCTC G[AT]GC[AT]^C 1800 GTGCTC Bsp1286I 1106 GTGCTC G[AGT]GC[ACT]^C 1800 GTGCTC BsrDI 1847 CATTGC GCAATG(2/0) 1956 CATTGC FbaI 938 TGATCA T^GATCA 1610 TGATCA MluNI 1502 TGGCCA TGG^CCA 1559 TGGCCA MscI 1502 TGGCCA TGG^CCA 1559 TGGCCA MseI 1115 TTAA T^TAA 1649 TTAA MslI 1480 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1543 CATAAAGGTG MspA1I 519 CAGCTG C[AC]G^C[GT]G 1155 CAGCGG NspI 1745 ACATGC [GA]CATG^[CT] 2084 ACATGT SfaNI 1291 GATGC GCATC(5/9) 815 GCATC SmlI 160 CTTGAG C^T[CT][GA]AG 1114 CTTAAG StuI 119 AGGCCT AGG^CCT 1668 AGGCCT TaqI 1130 TCGA T^CGA 2117 TCGA Tru9I 1115 TTAA T^TAA 1649 TTAA TthHB8I 1130 TCGA T^CGA 2117 TCGA XcmI 329 CCAGAGTGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1238 CCAAAGAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstUI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI TfiI TseI Tsp45I Tsp509I TspRI