(Circular) (Six-base) MAPSORT of: pFN21AE4566.seq Check: 7564 from: 1 to: 6835 pFN21AE4566 6835 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3075 4645 278 Size: 53 83 186 936 1539 1570 2468 Fragments arranged by size: 2468 1570 1539 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4122 4178 1937 Size: 2185 56 4594 Fragments arranged by size: 4594 2185 56 Acc65I G'GTAC_C Cuts at: 4156 4156 Size: 6835 AclI AA'CG_TT Cuts at: 1563 4962 5335 1563 Size: 3399 373 3063 Fragments arranged by size: 3399 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2000 2855 2941 2988 3776 3956 3974 4898 Size: 368 855 86 47 788 180 18 924 Cuts at: 4898 6282 6550 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 924 855 788 368 268 180 86 47 18 AflII C'TTAA_G Cuts at: 820 1017 3119 820 Size: 197 2102 4536 Fragments arranged by size: 4536 2102 197 AflIII A'CryG_T Cuts at: 1203 2028 4089 5734 6711 1203 Size: 825 2061 1645 977 1327 Fragments arranged by size: 2061 1645 1327 977 825 AgeI A'CCGG_T Cuts at: 5666 5666 Size: 6835 AhdI GACnn_n'nnGTC Cuts at: 5564 5564 Size: 6835 AleI CACnn'nnGTG Cuts at: 1166 3489 1166 Size: 2323 4512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4028 1184 Size: 2844 3991 AlwNI CAG_nnn'CTG Cuts at: 4578 6150 4578 Size: 1572 5263 ApaLI G'TGCA_C Cuts at: 4892 6048 4892 Size: 1156 5679 ApoI r'AATT_y Cuts at: 1493 2073 4144 4370 4522 6631 1493 Size: 580 2071 226 152 2109 1697 Fragments arranged by size: 2109 2071 1697 580 226 152 AseI AT'TA_AT Cuts at: 160 1032 3654 5389 160 Size: 872 2622 1735 1606 Fragments arranged by size: 2622 1735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 AvaI C'yCGr_G Cuts at: 1870 1943 4160 1870 Size: 73 2217 4545 Fragments arranged by size: 4545 2217 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3148 3181 3148 Size: 33 6802 BamHI G'GATC_C Cuts at: 2962 4165 4532 2962 Size: 1203 367 5265 Fragments arranged by size: 5265 1203 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 4110 4156 5611 618 Size: 325 217 627 696 1627 46 1455 1842 Fragments arranged by size: 1842 1627 1455 696 627 325 217 46 BanII G_rGCy'C Cuts at: 1391 4154 1391 Size: 2763 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 928 Size: 971 207 5657 Fragments arranged by size: 5657 971 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5026 5060 1903 Size: 34 50 34 3005 34 3678 Fragments arranged by size: 3678 3005 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4727 5937 3506 Size: 1221 1210 4404 Fragments arranged by size: 4404 1221 1210 BclI T'GATC_A Cuts at: 1548 2943 3615 1548 Size: 1395 672 4768 Fragments arranged by size: 4768 1395 672 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5446 136 Size: 107 122 71 631 605 3774 1525 Fragments arranged by size: 3774 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6830 6830 Size: 6835 BlpI GC'TnA_GC Cuts at: 4252 4252 Size: 6835 Bme1580I G_kGCm'C Cuts at: 1790 4896 6052 1790 Size: 3106 1156 2573 Fragments arranged by size: 3106 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2029 3543 1318 Size: 711 1514 4610 Fragments arranged by size: 4610 1514 711 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 4566 5524 Size: 416 53 83 399 24 1044 2105 958 Cuts at: 5524 442 Size: 1753 Fragments arranged by size: 2105 1753 1044 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6835 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4099 4099 Size: 6835 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3198 3275 5495 1239 Size: 1959 77 2220 2579 Fragments arranged by size: 2579 2220 1959 77 Bpu10I CC'TnA_GC Cuts at: 2997 2997 Size: 6835 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2186 4960 5825 6123 2186 Size: 2774 865 298 2898 Fragments arranged by size: 2898 2774 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 3697 5498 882 Size: 617 537 1661 1801 2219 Fragments arranged by size: 2219 1801 1661 617 537 BsaAI yAC'GTr Cuts at: 493 1204 2881 4024 4626 493 Size: 711 1677 1143 602 2702 Fragments arranged by size: 2702 1677 1143 711 602 BsaBI GATnn'nnATC Cuts at: 6829 6829 Size: 6835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 4642 5024 5659 Size: 53 83 186 936 1539 1570 382 635 Cuts at: 5659 275 Size: 1451 Fragments arranged by size: 1570 1539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5268 5666 5940 6087 6498 5268 Size: 398 274 147 411 5605 Fragments arranged by size: 5605 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6610 Fragments arranged by size: 6610 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3263 3534 3899 1186 Size: 2077 271 365 4122 Fragments arranged by size: 4122 2077 365 271 BseYI C'CCAG_C Cuts at: 6038 6534 6038 Size: 496 6339 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4285 5046 6074 1540 Size: 275 186 2284 761 1028 2301 Fragments arranged by size: 2301 2284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3115 3809 4896 4981 6052 3115 Size: 694 1087 85 1071 3898 Fragments arranged by size: 3898 1087 1071 694 85 BsmI GAATG_Cn' Cuts at: 4346 4439 4346 Size: 93 6742 BsmBI CGTCTCn'nnnn_ Cuts at: 3533 3533 Size: 6835 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3115 3809 4154 4896 4981 6052 1391 Size: 399 1325 694 345 742 85 1071 2174 Fragments arranged by size: 2174 1325 1071 742 694 399 345 85 BspEI T'CCGG_A Cuts at: 6498 6498 Size: 6835 BspHI T'CATG_A Cuts at: 2469 4724 6454 2469 Size: 2255 1730 2850 Fragments arranged by size: 2850 2255 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4190 844 Size: 3346 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3133 4722 1394 Size: 429 1310 1589 3507 Fragments arranged by size: 3507 1589 1310 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 3960 5330 5504 65 Size: 1183 2603 109 1370 174 1396 Fragments arranged by size: 2603 1396 1370 1183 174 109 BsrFI r'CCGG_y Cuts at: 1700 1810 5479 5666 1700 Size: 110 3669 187 2869 Fragments arranged by size: 3669 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2308 96 Size: 2212 4623 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6835 BssSI C'ACGA_G Cuts at: 4895 5907 4895 Size: 1012 5823 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6835 BstBI TT'CG_AA Cuts at: 5639 5639 Size: 6835 BstEII G'GTnAC_C Cuts at: 1184 3277 1184 Size: 2093 4742 BstXI CCAn_nnnn'nTGG Cuts at: 3049 3049 Size: 6835 BstYI r'GATC_y Cuts at: 1972 2962 4165 4532 4917 4934 6386 6472 6484 Size: 990 1203 367 385 17 1452 86 12 Cuts at: 6484 6830 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1203 990 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4042 513 Size: 552 2977 3306 Fragments arranged by size: 3306 2977 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2806 4087 487 Size: 683 56 1580 1281 3235 Fragments arranged by size: 3235 1580 1281 683 56 BtsI GCAGTG_nn' Cuts at: 2177 3510 3912 4351 5145 5165 2177 Size: 1333 402 439 794 20 3847 Fragments arranged by size: 3847 1333 794 439 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6800 DraI TTT'AAA Cuts at: 4139 4139 Size: 6835 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3935 5842 809 Size: 507 2619 1907 1802 Fragments arranged by size: 2619 1907 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2734 3507 3564 Size: 54 1425 50 275 18 904 773 57 Cuts at: 3564 3778 4282 5171 6800 8 Size: 214 504 889 1629 43 Fragments arranged by size: 1629 1425 904 889 773 504 275 214 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4282 1537 Size: 275 2470 4090 Fragments arranged by size: 4090 2470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 4765 1353 Size: 1611 1801 3423 Fragments arranged by size: 3423 1801 1611 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5422 5796 5942 387 Size: 1064 138 3833 374 146 1280 Fragments arranged by size: 3833 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2855 2941 2988 3198 3275 3776 Size: 393 368 855 86 47 210 77 501 Cuts at: 3776 3956 3974 4898 5495 6282 6550 1239 Size: 180 18 924 597 787 268 1524 Fragments arranged by size: 1524 924 855 787 597 501 393 368 268 210 180 86 77 47 18 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 6835 EcoRI G'AATT_C Cuts at: 4144 4522 4144 Size: 378 6457 FspI TGC'GCA Cuts at: 5341 5341 Size: 6835 HaeII r_GCGC'y Cuts at: 1678 5982 1678 Size: 4304 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3696 3728 5476 5508 5550 Size: 32 135 32 2124 32 1748 32 42 Cuts at: 5550 5582 1373 Size: 32 2626 Fragments arranged by size: 2626 2124 1748 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4123 4179 4425 669 Size: 988 281 2185 56 246 3079 Fragments arranged by size: 3079 2185 988 281 246 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 6835 HpaI GTT'AAC Cuts at: 4425 4425 Size: 6835 KpnI G_GTAC'C Cuts at: 4160 4160 Size: 6835 MfeI C'AATT_G Cuts at: 4434 4434 Size: 6835 MluI A'CGCG_T Cuts at: 6711 6711 Size: 6835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3066 3718 5949 6133 1305 Size: 1761 652 2231 184 2007 Fragments arranged by size: 2231 2007 1761 652 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 3566 10 Size: 54 1425 343 1677 57 3279 Fragments arranged by size: 3279 1677 1425 343 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 3489 3552 4793 5152 5311 518 Size: 648 2323 63 1241 359 159 2042 Fragments arranged by size: 2323 2042 1241 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2526 3162 4251 4928 6076 6321 1606 Size: 920 636 1089 677 1148 245 2120 Fragments arranged by size: 2120 1148 1089 920 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6283 NdeI CA'TA_TG Cuts at: 387 387 Size: 6835 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6835 NotI GC'GGCC_GC Cuts at: 4282 4282 Size: 6835 NspI r_CATG'y Cuts at: 1132 1229 3754 4093 4193 5738 1132 Size: 97 2525 339 100 1545 2229 Fragments arranged by size: 2525 2229 1545 339 100 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6835 PciI A'CATG_T Cuts at: 4089 5734 4089 Size: 1645 5190 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 5296 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 6835 PmeI GTTT'AAAC Cuts at: 4139 4139 Size: 6835 PmlI CAC'GTG Cuts at: 4024 4024 Size: 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4904 4936 6417 6449 4904 Size: 32 1481 32 5290 Fragments arranged by size: 5290 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6835 PsiI TTA'TAA Cuts at: 4405 4405 Size: 6835 PstI C_TGCA'G Cuts at: 830 3090 4187 830 Size: 2260 1097 3478 Fragments arranged by size: 3478 2260 1097 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6835 PvuII CAG'CTG Cuts at: 2526 2526 Size: 6835 SalI G'TCGA_C Cuts at: 1936 4121 4177 1936 Size: 2185 56 4594 Fragments arranged by size: 4594 2185 56 SbfI CC_TGCA'GG Cuts at: 4187 4187 Size: 6835 ScaI AGT'ACT Cuts at: 2988 5083 6795 2988 Size: 2095 1712 3028 Fragments arranged by size: 3028 2095 1712 SfcI C'TryA_G Cuts at: 826 1045 2801 3086 3454 4183 5318 5999 6190 Size: 219 1756 285 368 729 1135 681 191 Cuts at: 6190 826 Size: 1471 Fragments arranged by size: 1756 1471 1135 729 681 368 285 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6835 SmaI CCC'GGG Cuts at: 1872 4162 1872 Size: 2290 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3119 4939 5840 6102 820 Size: 197 926 222 954 1820 901 262 1553 Fragments arranged by size: 1820 1553 954 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 4626 493 Size: 4133 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6835 SphI G_CATG'C Cuts at: 1132 4193 1132 Size: 3061 3774 SspI AAT'ATT Cuts at: 5 52 2417 4759 5 Size: 47 2365 2342 2081 Fragments arranged by size: 2365 2342 2081 47 StuI AGG'CCT Cuts at: 1517 2126 3675 1517 Size: 609 1549 4677 Fragments arranged by size: 4677 1549 609 StyI C'CwwG_G Cuts at: 513 1065 4032 4274 513 Size: 552 2967 242 3074 Fragments arranged by size: 3074 2967 552 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 2699 3463 3479 3792 4862 4879 Size: 1255 863 256 764 16 313 1070 17 Cuts at: 4879 5032 5217 5662 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1070 863 764 445 313 256 185 153 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 2986 3975 Size: 274 80 33 51 1443 331 678 989 Cuts at: 3975 5081 6793 96 Size: 1106 1712 138 Fragments arranged by size: 1712 1443 1106 989 678 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3016 3048 6795 6827 3016 Size: 32 3747 32 3024 Fragments arranged by size: 3747 3024 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6835 XbaI T'CTAG_A Cuts at: 4171 4171 Size: 6835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 3250 2341 Size: 909 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6835 XmaI C'CCGG_G Cuts at: 1870 4160 1870 Size: 2290 4545 XmnI GAAnn'nnTTC Cuts at: 4964 4964 Size: 6835 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3073 4643 276 Size: 53 83 186 936 1539 1570 2468 Fragments arranged by size: 2468 1570 1539 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI