(Linear) (Six-base) MAPSORT of: KIEE4566.seq Check: 1617 from: 1 to: 2130 KIEE4566.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AatII G_ACGT'C Cuts at: 0 1070 2130 Size: 1070 1060 AccI GT'mk_AC Cuts at: 0 2117 2130 Size: 2117 13 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 936 983 1771 1951 1969 2130 Size: 850 86 47 788 180 18 161 Fragments arranged by size: 850 788 180 161 86 47 18 AflII C'TTAA_G Cuts at: 0 1114 2130 Size: 1114 1016 AflIII A'CryG_T Cuts at: 0 23 2084 2130 Size: 23 2061 46 Fragments arranged by size: 2061 46 23 AleI CACnn'nnGTG Cuts at: 0 1484 2130 Size: 1484 646 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2023 2130 Size: 2023 107 ApoI r'AATT_y Cuts at: 0 68 2130 Size: 68 2062 AseI AT'TA_AT Cuts at: 0 1649 2130 Size: 1649 481 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1143 1176 2130 Size: 1143 33 954 Fragments arranged by size: 1143 954 33 BamHI G'GATC_C Cuts at: 0 957 2130 Size: 957 1173 BanI G'GyrC_C Cuts at: 0 478 2105 2130 Size: 478 1627 25 Fragments arranged by size: 1627 478 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 101 2130 Size: 101 2029 BciVI GTATCCnnnnn_n' Cuts at: 0 1501 2130 Size: 1501 629 BclI T'GATC_A Cuts at: 0 938 1610 2130 Size: 938 672 520 Fragments arranged by size: 938 672 520 BmgBI CAC'GTC Cuts at: 0 24 1538 2130 Size: 24 1514 592 Fragments arranged by size: 1514 592 24 BmrI ACTGGGnnnn_n' Cuts at: 0 456 2130 Size: 456 1674 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2094 2130 Size: 2094 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1193 1270 2130 Size: 1193 77 860 Fragments arranged by size: 1193 860 77 Bpu10I CC'TnA_GC Cuts at: 0 992 2130 Size: 992 1138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 2130 Size: 181 1949 BsaI GGTCTCn'nnnn_ Cuts at: 0 31 1692 2130 Size: 31 1661 438 Fragments arranged by size: 1661 438 31 BsaAI yAC'GTr Cuts at: 0 876 2019 2130 Size: 876 1143 111 Fragments arranged by size: 1143 876 111 BsaHI Gr'CG_yC Cuts at: 0 1067 2130 Size: 1067 1063 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1258 1529 1894 2130 Size: 1258 271 365 236 Fragments arranged by size: 1258 365 271 236 BsiHKAI G_wGCw'C Cuts at: 0 1110 1804 2130 Size: 1110 694 326 Fragments arranged by size: 1110 694 326 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1528 2130 Size: 1528 602 Bsp1286I G_dGCh'C Cuts at: 0 1110 1804 2130 Size: 1110 694 326 Fragments arranged by size: 1110 694 326 BspHI T'CATG_A Cuts at: 0 464 2130 Size: 464 1666 BsrBI CCG'CTC Cuts at: 0 1128 2130 Size: 1128 1002 BsrDI GCAATG_nn' Cuts at: 0 1846 1955 2130 Size: 1846 109 175 Fragments arranged by size: 1846 175 109 BsrGI T'GTAC_A Cuts at: 0 303 2130 Size: 303 1827 BstEII G'GTnAC_C Cuts at: 0 1272 2130 Size: 1272 858 BstXI CCAn_nnnn'nTGG Cuts at: 0 1044 2130 Size: 1044 1086 BstYI r'GATC_y Cuts at: 0 957 2130 Size: 957 1173 BtgI C'CryG_G Cuts at: 0 2037 2130 Size: 2037 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 801 2082 2130 Size: 801 1281 48 Fragments arranged by size: 1281 801 48 BtsI GCAGTG_nn' Cuts at: 0 172 1505 1907 2130 Size: 172 1333 402 223 Fragments arranged by size: 1333 402 223 172 DrdI GACnn_nn'nnGTC Cuts at: 0 1930 2130 Size: 1930 200 EaeI y'GGCC_r Cuts at: 0 729 1502 1559 1773 2130 Size: 729 773 57 214 357 Fragments arranged by size: 773 729 357 214 57 EarI CTCTTCn'nnn_ Cuts at: 0 959 2130 Size: 959 1171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 936 983 1193 1270 1771 1951 1969 Size: 850 86 47 210 77 501 180 18 Cuts at: 1969 2130 Size: 161 Fragments arranged by size: 850 501 210 180 161 86 77 47 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 799 2130 Size: 799 1331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1691 1723 2130 Size: 1691 32 407 Fragments arranged by size: 1691 407 32 HincII GTy'rAC Cuts at: 0 2118 2130 Size: 2118 12 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1061 1713 2130 Size: 1061 652 417 Fragments arranged by size: 1061 652 417 MscI TGG'CCA Cuts at: 0 1504 1561 2130 Size: 1504 57 569 Fragments arranged by size: 1504 569 57 MslI CAynn'nnrTG Cuts at: 0 1484 1547 2130 Size: 1484 63 583 Fragments arranged by size: 1484 583 63 MspA1I CmG'CkG Cuts at: 0 521 1157 2130 Size: 521 636 973 Fragments arranged by size: 973 636 521 NspI r_CATG'y Cuts at: 0 1749 2088 2130 Size: 1749 339 42 Fragments arranged by size: 1749 339 42 PciI A'CATG_T Cuts at: 0 2084 2130 Size: 2084 46 PflMI CCAn_nnn'nTGG Cuts at: 0 642 2130 Size: 642 1488 PfoI T'CCnGG_A Cuts at: 0 338 2130 Size: 338 1792 PmlI CAC'GTG Cuts at: 0 2019 2130 Size: 2019 111 PstI C_TGCA'G Cuts at: 0 1085 2130 Size: 1085 1045 PvuII CAG'CTG Cuts at: 0 521 2130 Size: 521 1609 SalI G'TCGA_C Cuts at: 0 2116 2130 Size: 2116 14 ScaI AGT'ACT Cuts at: 0 983 2130 Size: 983 1147 SfcI C'TryA_G Cuts at: 0 796 1081 1449 2130 Size: 796 285 368 681 Fragments arranged by size: 796 681 368 285 SmlI C'TyrA_G Cuts at: 0 160 1114 2130 Size: 160 954 1016 Fragments arranged by size: 1016 954 160 SspI AAT'ATT Cuts at: 0 412 2130 Size: 412 1718 StuI AGG'CCT Cuts at: 0 121 1670 2130 Size: 121 1549 460 Fragments arranged by size: 1549 460 121 StyI C'CwwG_G Cuts at: 0 2027 2130 Size: 2027 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 438 694 1458 1474 1787 2130 Size: 438 256 764 16 313 343 Fragments arranged by size: 764 438 343 313 256 16 TatI w'GTAC_w Cuts at: 0 303 981 1970 2130 Size: 303 678 989 160 Fragments arranged by size: 989 678 303 160 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1011 1043 2130 Size: 1011 32 1087 Fragments arranged by size: 1087 1011 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 336 1245 2130 Size: 336 909 885 Fragments arranged by size: 909 885 336 ZraI GAC'GTC Cuts at: 0 1068 2130 Size: 1068 1062 Enzymes that do cut: AatII AccI AcuI AflII AflIII AleI AlfI ApoI AseI BaeI BamHI BanI BbsI BciVI BclI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI BstEII BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoNI Hin4I HincII MmeI MscI MslI MspA1I NspI PciI PflMI PfoI PmlI PstI PvuII SalI ScaI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BanII BbeI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmtI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI