(Circular) (Six-base) MAPSORT of: pFN21AE5409.seq Check: 8749 from: 1 to: 5752 pFN21AE5409 5752 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2694 3095 1937 Size: 757 401 4594 Fragments arranged by size: 4594 757 401 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2898 3815 5199 5467 1632 Size: 600 666 917 1384 268 1917 Fragments arranged by size: 1917 1384 917 666 600 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 3047 4651 5628 1203 Size: 1844 1604 977 1327 Fragments arranged by size: 1844 1604 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 2294 2690 3495 5067 2294 Size: 396 805 1572 2979 Fragments arranged by size: 2979 1572 805 396 ApaI G_GGCC'C Cuts at: 2244 2244 Size: 5752 ApaLI G'TGCA_C Cuts at: 2225 3809 4965 2225 Size: 1584 1156 3012 Fragments arranged by size: 3012 1584 1156 ApoI r'AATT_y Cuts at: 1493 2567 2714 3061 3287 3439 5548 1493 Size: 1074 147 347 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 347 226 152 147 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2376 3077 1870 Size: 73 433 701 4545 Fragments arranged by size: 4545 701 433 73 BamHI G'GATC_C Cuts at: 2156 3082 3449 2156 Size: 926 367 4459 Fragments arranged by size: 4459 926 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 2244 3071 1391 Size: 853 827 4072 Fragments arranged by size: 4072 853 827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 2886 928 Size: 971 126 861 3794 Fragments arranged by size: 3794 971 861 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2186 2239 4363 Size: 107 122 71 631 605 514 53 2124 Cuts at: 4363 136 Size: 1525 Fragments arranged by size: 2124 1525 631 605 514 122 107 71 53 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2229 2244 2595 3813 4969 1790 Size: 439 15 351 1218 1156 2573 Fragments arranged by size: 2573 1218 1156 439 351 15 BmgBI CAC'GTC Cuts at: 1318 3048 1318 Size: 1730 4022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2477 3483 4441 Size: 416 53 83 399 24 1060 1006 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1060 1006 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 4412 1239 Size: 1053 2120 2579 Fragments arranged by size: 2579 2120 1053 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2227 2936 3877 4742 5040 2227 Size: 709 941 865 298 2939 Fragments arranged by size: 2939 941 865 709 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2176 4415 882 Size: 617 677 2239 2219 Fragments arranged by size: 2239 2219 677 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2153 4185 4583 4857 5004 5415 2153 Size: 2032 398 274 147 411 2490 Fragments arranged by size: 2490 2032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2373 2403 2687 2717 1178 Size: 30 165 30 970 30 284 30 4213 Fragments arranged by size: 4213 970 284 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2394 2769 1186 Size: 1208 375 4169 Fragments arranged by size: 4169 1208 375 BseYI C'CCAG_C Cuts at: 2938 4955 5451 2938 Size: 2017 496 3239 Fragments arranged by size: 3239 2017 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2142 3202 3963 4991 1540 Size: 275 186 87 54 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 87 54 BsiHKAI G_wGCw'C Cuts at: 2229 3813 3898 4969 2229 Size: 1584 85 1071 3012 Fragments arranged by size: 3012 1584 1071 85 BsmI GAATG_Cn' Cuts at: 2304 2556 3263 3356 2304 Size: 252 707 93 4700 Fragments arranged by size: 4700 707 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2244 2595 3071 3813 3898 4969 Size: 399 439 15 351 476 742 85 1071 Cuts at: 4969 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 476 439 399 351 85 15 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2683 3107 844 Size: 1839 424 3489 Fragments arranged by size: 3489 1839 424 BsrBI CCG'CTC Cuts at: 1394 1823 2211 3639 1394 Size: 429 388 1428 3507 Fragments arranged by size: 3507 1428 429 388 BsrDI GCAATG_nn' Cuts at: 65 1248 2100 2901 2920 4247 4421 65 Size: 1183 852 801 19 1327 174 1396 Fragments arranged by size: 1396 1327 1183 852 801 174 19 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 2099 2120 2675 794 Size: 1305 21 555 3871 Fragments arranged by size: 3871 1305 555 21 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2156 3082 3449 3834 3851 5303 5389 5401 Size: 184 926 367 385 17 1452 86 12 Cuts at: 5401 5747 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 926 385 367 346 184 86 17 12 BstZ17I GTA'TAC Cuts at: 2695 2695 Size: 5752 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2142 513 Size: 552 939 123 15 4123 Fragments arranged by size: 4123 939 552 123 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2139 2145 Size: 54 1425 50 275 18 177 132 6 Cuts at: 2145 3199 4088 5717 8 Size: 1054 889 1629 43 Fragments arranged by size: 1629 1425 1054 889 275 177 132 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2139 3199 1537 Size: 275 327 1060 4090 Fragments arranged by size: 4090 1060 327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 3682 1353 Size: 1289 1040 3423 Fragments arranged by size: 3423 1289 1040 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2292 2898 3815 4412 5199 5467 Size: 393 600 60 606 917 597 787 268 Cuts at: 5467 1239 Size: 1524 Fragments arranged by size: 1524 917 787 606 600 597 393 268 60 EcoO109I rG'GnC_Cy Cuts at: 2240 2240 Size: 5752 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2643 2675 4393 4425 4467 Size: 32 135 32 1071 32 1718 32 42 Cuts at: 4467 4499 1373 Size: 32 2626 Fragments arranged by size: 2626 1718 1071 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 3096 3342 669 Size: 988 281 483 675 246 3079 Fragments arranged by size: 3079 988 675 483 281 246 HindIII A'AGCT_T Cuts at: 748 2773 748 Size: 2025 3727 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 2892 3351 2892 Size: 459 5293 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2374 4866 5050 1305 Size: 1069 2492 184 2007 Fragments arranged by size: 2492 2007 1069 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3710 4069 4228 518 Size: 648 2544 359 159 2042 Fragments arranged by size: 2544 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2129 2144 3168 3845 4993 5238 1606 Size: 523 15 1024 677 1148 245 2120 Fragments arranged by size: 2120 1148 1024 677 523 245 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4261 Fragments arranged by size: 4261 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 2139 3199 2139 Size: 1060 4692 NspI r_CATG'y Cuts at: 1132 1229 2870 3110 4655 1132 Size: 97 1641 240 1545 2229 Fragments arranged by size: 2229 1641 1545 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PspOMI G'GGCC_C Cuts at: 2240 2240 Size: 5752 PstI C_TGCA'G Cuts at: 830 2080 2125 3104 830 Size: 1250 45 979 3478 Fragments arranged by size: 3478 1250 979 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 SacII CC_GC'GG Cuts at: 2130 2145 2130 Size: 15 5737 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2076 2121 2561 3100 4235 4916 5107 Size: 219 1031 45 440 539 1135 681 191 Cuts at: 5107 826 Size: 1471 Fragments arranged by size: 1471 1135 1031 681 539 440 219 191 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2206 2915 3856 4757 5019 820 Size: 197 926 263 709 941 901 262 1553 Fragments arranged by size: 1553 941 926 901 709 263 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 3191 513 Size: 552 939 171 1016 3074 Fragments arranged by size: 3074 1016 939 552 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 3796 3949 4134 4579 325 Size: 1255 2199 17 153 185 445 1498 Fragments arranged by size: 2199 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3998 5710 96 Size: 274 80 33 51 1443 2021 1712 138 Fragments arranged by size: 2021 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 2107 1316 Size: 791 4961 XbaI T'CTAG_A Cuts at: 2362 3088 2362 Size: 726 5026 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2464 2182 Size: 282 5470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 2302 2556 3881 2302 Size: 254 1325 4173 Fragments arranged by size: 4173 1325 254 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI