Restriction map for
pFN21AE5409
(Linear) (Six-base) MAPSORT of: KIEE5409.seq Check: 2734 from: 1 to: 1047
KIEE5409.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AccI GT'mk_AC
Cuts at: 0 689 1047
Size: 689 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 893 1047
Size: 227 666 154
Fragments arranged by size:
666 227 154
AflIII A'CryG_T
Cuts at: 0 1042 1047
Size: 1042 5
AlwNI CAG_nnn'CTG
Cuts at: 0 289 685 1047
Size: 289 396 362
Fragments arranged by size:
396 362 289
ApaI G_GGCC'C
Cuts at: 0 239 1047
Size: 239 808
ApaLI G'TGCA_C
Cuts at: 0 220 1047
Size: 220 827
ApoI r'AATT_y
Cuts at: 0 562 709 1047
Size: 562 147 338
Fragments arranged by size:
562 338 147
AvaI C'yCGr_G
Cuts at: 0 371 1047
Size: 371 676
BamHI G'GATC_C
Cuts at: 0 151 1047
Size: 151 896
BanII G_rGCy'C
Cuts at: 0 239 1047
Size: 239 808
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 881 1047
Size: 20 861 166
Fragments arranged by size:
861 166 20
BglI GCCn_nnn'nGGC
Cuts at: 0 181 234 1047
Size: 181 53 813
Fragments arranged by size:
813 181 53
Bme1580I G_kGCm'C
Cuts at: 0 224 239 590 1047
Size: 224 15 351 457
Fragments arranged by size:
457 351 224 15
BmgBI CAC'GTC
Cuts at: 0 1043 1047
Size: 1043 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 472 1047
Size: 472 575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1047
Size: 936 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1047
Size: 287 760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 931 1047
Size: 222 709 116
Fragments arranged by size:
709 222 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 171 1047
Size: 171 876
BsaWI w'CCGG_w
Cuts at: 0 148 1047
Size: 148 899
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 368 398 682 712 1047
Size: 368 30 284 30 335
Fragments arranged by size:
368 335 284 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 389 764 1047
Size: 389 375 283
Fragments arranged by size:
389 375 283
BseYI C'CCAG_C
Cuts at: 0 933 1047
Size: 933 114
BsiEI CG_ry'CG
Cuts at: 0 83 137 1047
Size: 83 54 910
Fragments arranged by size:
910 83 54
BsiHKAI G_wGCw'C
Cuts at: 0 224 1047
Size: 224 823
BsmI GAATG_Cn'
Cuts at: 0 299 551 1047
Size: 299 252 496
Fragments arranged by size:
496 299 252
Bsp1286I G_dGCh'C
Cuts at: 0 224 239 590 1047
Size: 224 15 351 457
Fragments arranged by size:
457 351 224 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 1047
Size: 678 369
BsrBI CCG'CTC
Cuts at: 0 206 1047
Size: 206 841
BsrDI GCAATG_nn'
Cuts at: 0 95 896 915 1047
Size: 95 801 19 132
Fragments arranged by size:
801 132 95 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 94 115 670 1047
Size: 94 21 555 377
Fragments arranged by size:
555 377 94 21
BstYI r'GATC_y
Cuts at: 0 151 1047
Size: 151 896
BstZ17I GTA'TAC
Cuts at: 0 690 1047
Size: 690 357
BtgI C'CryG_G
Cuts at: 0 122 137 1047
Size: 122 15 910
Fragments arranged by size:
910 122 15
EaeI y'GGCC_r
Cuts at: 0 2 134 140 1047
Size: 2 132 6 907
Fragments arranged by size:
907 132 6 2
EagI C'GGCC_G
Cuts at: 0 134 1047
Size: 134 913
EarI CTCTTCn'nnn_
Cuts at: 0 637 1047
Size: 637 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 287 893 1047
Size: 227 60 606 154
Fragments arranged by size:
606 227 154 60
EcoO109I rG'GnC_Cy
Cuts at: 0 235 1047
Size: 235 812
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 1047
Size: 638 32 377
Fragments arranged by size:
638 377 32
HincII GTy'rAC
Cuts at: 0 416 1047
Size: 416 631
HindIII A'AGCT_T
Cuts at: 0 768 1047
Size: 768 279
MfeI C'AATT_G
Cuts at: 0 887 1047
Size: 887 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1047
Size: 369 678
MspA1I CmG'CkG
Cuts at: 0 124 139 1047
Size: 124 15 908
Fragments arranged by size:
908 124 15
NotI GC'GGCC_GC
Cuts at: 0 134 1047
Size: 134 913
NspI r_CATG'y
Cuts at: 0 865 1047
Size: 865 182
PspOMI G'GGCC_C
Cuts at: 0 235 1047
Size: 235 812
PstI C_TGCA'G
Cuts at: 0 75 120 1047
Size: 75 45 927
Fragments arranged by size:
927 75 45
SacII CC_GC'GG
Cuts at: 0 125 140 1047
Size: 125 15 907
Fragments arranged by size:
907 125 15
SfcI C'TryA_G
Cuts at: 0 71 116 556 1047
Size: 71 45 440 491
Fragments arranged by size:
491 440 71 45
SmlI C'TyrA_G
Cuts at: 0 201 910 1047
Size: 201 709 137
Fragments arranged by size:
709 201 137
StyI C'CwwG_G
Cuts at: 0 170 1047
Size: 170 877
Tth111I GACn'n_nGTC
Cuts at: 0 102 1047
Size: 102 945
XbaI T'CTAG_A
Cuts at: 0 357 1047
Size: 357 690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 459 1047
Size: 177 282 588
Fragments arranged by size:
588 282 177
XmnI GAAnn'nnTTC
Cuts at: 0 297 551 1047
Size: 297 254 496
Fragments arranged by size:
496 297 254
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI
BamHI BanII BbsI BglI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstYI
BstZ17I BtgI EaeI EagI EarI Eco57MI EcoO109I Hin4I
HincII HindIII MfeI MmeI MspA1I NotI NspI PspOMI
PstI SacII SfcI SmlI StyI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI Bpu10I BsaAI BsaBI BsaHI BsgI BsiWI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XhoI XmaI ZraI