Restriction map for
pFN21AB8544
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BanI
BbeI BcgI BfrI BglI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2654 GACGTC GACGT^C
AccI 2277 GTCTAC GT^[AC][GT]AC
AcyI 2654 GACGTC G[GA]^CG[CT]C
AlwNI 278 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 2320 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1245 CCTAGG C^CTAGG
BalI 704 TGGCCA TGG^CCA
BbvCI 195 GCTGAGG CCTCAGC(-5/-2)
BciVI 1942 GTATCC GTATCC(6/5)
BclI 432 TGATCA T^GATCA
BcnI 1975 CCGGG CC^[GC]GG
BglII 2625 AGATCT A^GATCT
BlnI 1245 CCTAGG C^CTAGG
BlpI 451 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC
BsaHI 2654 GACGTC G[GA]^CG[CT]C
BsmI 1669 GAATGC GAATGC(1/-1)
Bsp1407I 2074 TGTACA T^GTACA
BspDI 1484 ATCGAT AT^CGAT
BsrFI 521 ACCGGC [GA]^CCGG[CT]
BsrGI 2074 TGTACA T^GTACA
CelII 451 GCTGAGC GC^T[ACGT]AGC
Cfr10I 521 ACCGGC [GA]^CCGG[CT]
ClaI 1484 ATCGAT AT^CGAT
DraIII 586 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1857 CCATGG C^C[GA][CT]GG
EaeI 704 TGGCCA [CT]^GGCC[GA]
EcoRI 172 GAATTC G^AATTC
EcoT22I 1671 ATGCAT ATGCA^T
FbaI 432 TGATCA T^GATCA
HgaI 1503 GCGTC GACGC(5/10)
Hin1I 2654 GACGTC G[GA]^CG[CT]C
HincII 517 GTTAAC GT[CT]^[GA]AC
HindII 517 GTTAAC GT[CT]^[GA]AC
HpaI 517 GTTAAC GTT^AAC
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
MwoI 20 GCTGTGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1975 CCGGG CC^[GC]GG
NcoI 1857 CCATGG C^CATGG
NdeI 292 CATATG CA^TATG
NsiI 1671 ATGCAT ATGCA^T
PaeR7I 968 CTCGAG C^TCGAG
PflFI 2320 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 849 CCACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 685 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 38 CTGCAG CTGCA^G
SacI 841 GAGCTC GAGCT^C
SexAI 1846 ACCAGGT A^CC[AT]GGT
SspBI 2074 TGTACA T^GTACA
SspI 2673 AATATT AAT^ATT
StuI 1610 AGGCCT AGG^CCT
Tth111I 2320 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 849 CCACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1108 TCTAGA T^CTAGA
XcmI 2557 CCAGAGAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 968 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 2535 CCGC CCGC(-3/-1)
297 GCGG
AflIII 2447 ACACGT A^C[GA][CT]GT
2449 ACGTGT
Asp700I 611 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2089 GAAACCGTTC
AspHI 583 GAGCAC G[AT]GC[AT]^C
841 GAGCTC
AvaI 9 CTCGGG C^[CT]CG[GA]G
968 CTCGAG
AvaII 63 GGACC G^G[AT]CC
817 GGTCC
BbrPI 1769 CACGTG CAC^GTG
2448 CACGTG
BpmI 412 CTCCAG CTGGAG(16/14)
1703 CTCCAG
BsaAI 1769 CACGTG [CT]AC^GT[GA]
2448 CACGTG
BsgI 1626 CTGCAC GTGCAG(16/14)
275 GTGCAG
BsiHKAI 583 GAGCAC G[AT]GC[AT]^C
841 GAGCTC
BsiYI 849 CCACTGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
982 CCTGTATTTGG
BslI 849 CCACTGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
982 CCTGTATTTGG
BsmFI 818 GTCCC GGGAC(10/14)
1038 GTCCC
BsoBI 9 CTCGGG C^[CT]CG[GA]G
968 CTCGAG
Cfr13I 63 GGACC G^G[ACGT]CC
817 GGTCC
DraI 1750 TTTAAA TTT^AAA
2356 TTTAAA
DraII 62 AGGACCT [GA]G^G[ACGT]CC[CT]
816 AGGTCCC
EarI 2215 GAAGAG CTCTTC(1/4)
2263 GAAGAG
EcoO109I 62 AGGACCT [GA]G^G[ACGT]CC[CT]
816 AGGTCCC
HindIII 397 AAGCTT A^AGCTT
1385 AAGCTT
Ksp632I 2215 GAAGAG CTCTTC(1/4)
2263 GAAGAG
MslI 1664 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2299 CACACTCATG
PleI 942 GACTC GAGTC(4/5)
1557 GACTC
PmaCI 1769 CACGTG CAC^GTG
2448 CACGTG
PmlI 1769 CACGTG CAC^GTG
2448 CACGTG
PpuMI 62 AGGACCT [GA]G^G[AT]CC[CT]
816 AGGTCCC
Sau96I 63 GGACC G^G[ACGT]CC
817 GGTCC
TaqI 969 TCGA T^CGA
1485 TCGA
Tsp45I 53 GTGAC ^GT[GC]AC
1555 GTGAC
TthHB8I 969 TCGA T^CGA
1485 TCGA
XmnI 611 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2089 GAAACCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanII BbsI BbvI BfaI BmyI
BpuAI BsaBI BsaJI BseRI BsmAI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI NdeII NlaIII NlaIV
PvuII RcaI RsaI Sau3AI ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp509I TspRI XhoII