(Circular) (Six-base) MAPSORT of: pFN21AB8544.seq Check: 2172 from: 1 to: 7411 pFN21AB8544 7411 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4663 5221 278 Size: 53 83 186 936 3127 558 2468 Fragments arranged by size: 3127 2468 936 558 186 83 53 AccI GT'mk_AC Cuts at: 1937 4283 4754 1937 Size: 2346 471 4594 Fragments arranged by size: 4594 2346 471 Acc65I G'GTAC_C Cuts at: 4732 4732 Size: 7411 AclI AA'CG_TT Cuts at: 1563 5538 5911 1563 Size: 3975 373 3063 Fragments arranged by size: 3975 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2931 3162 3201 3671 3875 5474 6858 Size: 678 621 231 39 470 204 1599 1384 Cuts at: 6858 7126 1632 Size: 268 1917 Fragments arranged by size: 1917 1599 1384 678 621 470 268 231 204 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7214 AflIII A'CryG_T Cuts at: 1203 4452 4454 6310 7287 1203 Size: 3249 2 1856 977 1327 Fragments arranged by size: 3249 1856 1327 977 2 AgeI A'CCGG_T Cuts at: 6242 6242 Size: 7411 AhdI GACnn_n'nnGTC Cuts at: 6140 6140 Size: 7411 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7411 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7411 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2781 2813 2781 Size: 32 7379 AlwNI CAG_nnn'CTG Cuts at: 2288 5154 6726 2288 Size: 2866 1572 2973 Fragments arranged by size: 2973 2866 1572 ApaLI G'TGCA_C Cuts at: 5468 6624 5468 Size: 1156 6255 ApoI r'AATT_y Cuts at: 1493 2177 2667 3548 3759 4393 4470 4567 4720 Size: 684 490 881 211 634 77 97 153 Cuts at: 4720 4946 5098 7207 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 684 634 490 226 211 153 152 97 77 AseI AT'TA_AT Cuts at: 160 1032 5965 160 Size: 872 4933 1606 Fragments arranged by size: 4933 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2973 4736 1870 Size: 73 71 959 1763 4545 Fragments arranged by size: 4545 1763 959 73 71 AvrII C'CTAG_G Cuts at: 3250 3250 Size: 7411 BamHI G'GATC_C Cuts at: 4741 5108 4741 Size: 367 7044 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4732 6187 618 Size: 325 217 627 2945 1455 1842 Fragments arranged by size: 2945 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2834 2850 3312 3663 4730 1391 Size: 1443 16 462 351 1067 4072 Fragments arranged by size: 4072 1443 1067 462 351 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 2622 3587 4712 928 Size: 971 582 141 965 1125 3627 Fragments arranged by size: 3627 1125 971 965 582 141 BbvCI CC'TCA_GC Cuts at: 2201 2201 Size: 7411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5602 5636 1903 Size: 34 3665 34 3678 Fragments arranged by size: 3678 3665 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3958 5303 6513 3958 Size: 1345 1210 4856 Fragments arranged by size: 4856 1345 1210 BclI T'GATC_A Cuts at: 1548 2437 1548 Size: 889 6522 BfrBI ATG'CAT Cuts at: 3678 3678 Size: 7411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6022 136 Size: 107 122 71 631 605 4350 1525 Fragments arranged by size: 4350 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4630 7406 4630 Size: 2776 4635 BlpI GC'TnA_GC Cuts at: 2457 4828 2457 Size: 2371 5040 Bme1580I G_kGCm'C Cuts at: 1790 3154 4452 5472 6628 1790 Size: 1364 1298 1020 1156 2573 Fragments arranged by size: 2573 1364 1298 1156 1020 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5142 6100 442 Size: 416 53 83 399 24 3725 958 1753 Fragments arranged by size: 3725 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 3693 6071 1239 Size: 1163 1291 2378 2579 Fragments arranged by size: 2579 2378 1291 1163 Bpu10I CC'TnA_GC Cuts at: 2201 2201 Size: 7411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2802 2940 3589 4510 5536 6401 6699 2802 Size: 138 649 921 1026 865 298 3514 Fragments arranged by size: 3514 1026 921 865 649 298 138 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6074 882 Size: 617 4575 2219 Fragments arranged by size: 4575 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3776 4455 5202 493 Size: 711 2572 679 747 2702 Fragments arranged by size: 2702 2572 747 711 679 BsaBI GATnn'nnATC Cuts at: 3136 3197 4635 7405 3136 Size: 61 1438 2770 3142 Fragments arranged by size: 3142 2770 1438 61 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4660 5218 5600 6235 Size: 53 83 186 936 3127 558 382 635 Cuts at: 6235 275 Size: 1451 Fragments arranged by size: 3127 1451 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5844 6242 6516 6663 7074 5844 Size: 398 274 147 411 6181 Fragments arranged by size: 6181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3444 3474 4050 4080 4235 Size: 30 165 30 2041 30 576 30 155 Cuts at: 4235 4265 4295 1178 Size: 30 30 4294 Fragments arranged by size: 4294 2041 576 165 155 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2940 2943 4071 4286 4442 1186 Size: 1754 3 1128 215 156 4155 Fragments arranged by size: 4155 1754 1128 215 156 3 BseYI C'CCAG_C Cuts at: 6614 7110 6614 Size: 496 6915 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2301 3616 1163 Size: 573 565 1315 4958 Fragments arranged by size: 4958 1315 573 565 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4861 5622 6650 1540 Size: 275 186 2860 761 1028 2301 Fragments arranged by size: 2860 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2592 2850 5472 5557 6628 2592 Size: 258 2622 85 1071 3375 Fragments arranged by size: 3375 2622 1071 258 85 BsmI GAATG_Cn' Cuts at: 3680 4922 5015 3680 Size: 1242 93 6076 Fragments arranged by size: 6076 1242 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2592 2834 2850 3154 3312 3663 4452 Size: 399 802 242 16 304 158 351 789 Cuts at: 4452 4730 5472 5557 6628 1391 Size: 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 802 789 742 399 351 304 278 242 158 85 16 BspEI T'CCGG_A Cuts at: 7074 7074 Size: 7411 BspHI T'CATG_A Cuts at: 2095 2224 3829 5300 7030 2095 Size: 129 1605 1471 1730 2476 Fragments arranged by size: 2476 1730 1605 1471 129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4766 844 Size: 3922 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5298 1394 Size: 429 3475 3507 Fragments arranged by size: 3507 3475 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5906 6080 65 Size: 1183 4658 174 1396 Fragments arranged by size: 4658 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 6055 6242 1700 Size: 110 716 3529 187 2869 Fragments arranged by size: 3529 2869 716 187 110 BsrGI T'GTAC_A Cuts at: 96 4079 96 Size: 3983 3428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7411 BssSI C'ACGA_G Cuts at: 5471 6483 5471 Size: 1012 6399 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7411 BstBI TT'CG_AA Cuts at: 6215 6215 Size: 7411 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7411 BstYI r'GATC_y Cuts at: 1972 3478 3976 4630 4741 5108 5493 5510 6962 Size: 1506 498 654 111 367 385 17 1452 Cuts at: 6962 7048 7060 7406 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1506 1452 654 498 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3862 513 Size: 552 2797 4062 Fragments arranged by size: 4062 2797 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6672 Fragments arranged by size: 6672 683 56 BtsI GCAGTG_nn' Cuts at: 4927 5721 5741 4927 Size: 794 20 6597 Fragments arranged by size: 6597 794 20 ClaI AT'CG_AT Cuts at: 1554 3490 1554 Size: 1936 5475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7376 DraI TTT'AAA Cuts at: 3757 4363 4715 3757 Size: 606 352 6453 Fragments arranged by size: 6453 606 352 DraIII CAC_nnn'GTG Cuts at: 2596 2596 Size: 7411 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6418 809 Size: 507 5102 1802 Fragments arranged by size: 5102 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2709 4858 5747 Size: 54 1425 50 275 18 879 2149 889 Cuts at: 5747 7376 8 Size: 1629 43 Fragments arranged by size: 2149 1629 1425 889 879 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4858 1537 Size: 275 3046 4090 Fragments arranged by size: 4090 3046 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4215 4263 5341 1353 Size: 2862 48 1078 3423 Fragments arranged by size: 3423 2862 1078 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5998 6372 6518 387 Size: 1064 138 4409 374 146 1280 Fragments arranged by size: 4409 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2848 2848 Size: 7411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2402 2931 3162 3201 3671 3693 Size: 393 678 92 529 231 39 470 22 Cuts at: 3693 3875 5474 6071 6858 7126 1239 Size: 182 1599 597 787 268 1524 Fragments arranged by size: 1599 1524 787 678 597 529 470 393 268 231 182 92 39 22 EcoO109I rG'GnC_Cy Cuts at: 2068 2822 2068 Size: 754 6657 EcoRI G'AATT_C Cuts at: 2177 4720 5098 2177 Size: 2543 378 4490 Fragments arranged by size: 4490 2543 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3552 3581 3552 Size: 29 7382 FspI TGC'GCA Cuts at: 5917 5917 Size: 7411 HaeII r_GCGC'y Cuts at: 1678 6558 1678 Size: 4880 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 2938 2970 3961 Size: 32 135 32 1163 32 171 32 991 Cuts at: 3961 3993 4214 4235 4246 4265 4267 4297 6052 Size: 32 221 21 11 19 2 30 1755 Cuts at: 6052 6084 6126 6158 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1755 1163 991 221 171 135 42 32 32 32 32 32 32 32 30 21 19 11 2 HincII GTy'rAC Cuts at: 669 1657 1938 2524 4755 5001 669 Size: 988 281 586 2231 246 3079 Fragments arranged by size: 3079 2231 988 586 281 246 HindIII A'AGCT_T Cuts at: 748 2402 3390 748 Size: 1654 988 4769 Fragments arranged by size: 4769 1654 988 HpaI GTT'AAC Cuts at: 2524 5001 2524 Size: 2477 4934 KpnI G_GTAC'C Cuts at: 4736 4736 Size: 7411 MfeI C'AATT_G Cuts at: 5010 5010 Size: 7411 MluI A'CGCG_T Cuts at: 7287 7287 Size: 7411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6525 6709 1305 Size: 5220 184 2007 Fragments arranged by size: 5220 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 4710 Fragments arranged by size: 4710 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3673 4308 5369 5728 5887 518 Size: 648 2507 635 1061 359 159 2042 Fragments arranged by size: 2507 2042 1061 648 635 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2200 2456 4827 5504 6652 6897 1606 Size: 419 175 256 2371 677 1148 245 2120 Fragments arranged by size: 2371 2120 1148 677 419 256 245 175 NcoI C'CATG_G Cuts at: 513 1065 3862 513 Size: 552 2797 4062 Fragments arranged by size: 4062 2797 552 NdeI CA'TA_TG Cuts at: 387 2298 387 Size: 1911 5500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7411 NotI GC'GGCC_GC Cuts at: 4858 4858 Size: 7411 NsiI A_TGCA'T Cuts at: 3680 3680 Size: 7411 NspI r_CATG'y Cuts at: 1132 1229 4769 6314 1132 Size: 97 3540 1545 2229 Fragments arranged by size: 3540 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3653 1348 Size: 2305 5106 PciI A'CATG_T Cuts at: 6310 6310 Size: 7411 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 1108 Size: 1752 5659 PfoI T'CCnGG_A Cuts at: 3979 3979 Size: 7411 PmeI GTTT'AAAC Cuts at: 4715 4715 Size: 7411 PmlI CAC'GTG Cuts at: 3776 4455 3776 Size: 679 6732 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3808 3840 5480 5512 6993 7025 3808 Size: 32 1640 32 1481 32 4194 Fragments arranged by size: 4194 1640 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2822 2068 Size: 754 6657 PshAI GACnn'nnGTC Cuts at: 1613 2694 1613 Size: 1081 6330 PsiI TTA'TAA Cuts at: 3227 4981 3227 Size: 1754 5657 PspXI vC'TCGA_Gb Cuts at: 2973 2973 Size: 7411 PstI C_TGCA'G Cuts at: 830 2047 4763 830 Size: 1217 2716 3478 Fragments arranged by size: 3478 2716 1217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7411 PvuII CAG'CTG Cuts at: 2025 2200 2456 2025 Size: 175 256 6980 Fragments arranged by size: 6980 256 175 SacI G_AGCT'C Cuts at: 2850 2850 Size: 7411 SalI G'TCGA_C Cuts at: 1936 4753 1936 Size: 2817 4594 SbfI CC_TGCA'GG Cuts at: 4763 4763 Size: 7411 ScaI AGT'ACT Cuts at: 5659 7371 5659 Size: 1712 5699 SexAI A'CCwGG_T Cuts at: 3851 3851 Size: 7411 SfcI C'TryA_G Cuts at: 826 1045 2043 2384 2766 4759 5894 6575 6766 Size: 219 998 341 382 1993 1135 681 191 Cuts at: 6766 826 Size: 1471 Fragments arranged by size: 1993 1471 1135 998 681 382 341 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7411 SmaI CCC'GGG Cuts at: 1872 4738 1872 Size: 2866 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2817 2955 2973 3604 4525 5515 Size: 197 926 874 138 18 631 921 990 Cuts at: 5515 6416 6678 820 Size: 901 262 1553 Fragments arranged by size: 1553 990 926 921 901 874 631 262 197 138 18 SnaBI TAC'GTA Cuts at: 493 5202 493 Size: 4709 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7411 SphI G_CATG'C Cuts at: 1132 4769 1132 Size: 3637 3774 SspI AAT'ATT Cuts at: 5 52 4680 5335 5 Size: 47 4628 655 2081 Fragments arranged by size: 4628 2081 655 47 StuI AGG'CCT Cuts at: 1517 3617 1517 Size: 2100 5311 StyI C'CwwG_G Cuts at: 513 1065 2836 3250 3523 3862 4850 513 Size: 552 1771 414 273 339 988 3074 Fragments arranged by size: 3074 1771 988 552 414 339 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4567 5438 5455 5608 5793 6238 325 Size: 1255 2987 871 17 153 185 445 1498 Fragments arranged by size: 2987 1498 1255 871 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2107 4079 5657 Size: 274 80 33 51 1443 130 1972 1578 Cuts at: 5657 7369 96 Size: 1712 138 Fragments arranged by size: 1972 1712 1578 1443 274 138 130 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3444 3476 4050 4082 7371 7403 3444 Size: 32 574 32 3289 32 3452 Fragments arranged by size: 3452 3289 574 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4328 1316 Size: 3012 4399 XbaI T'CTAG_A Cuts at: 3113 4747 3113 Size: 1634 5777 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4569 4569 Size: 7411 XhoI C'TCGA_G Cuts at: 1943 2973 1943 Size: 1030 6381 XmaI C'CCGG_G Cuts at: 1870 4736 1870 Size: 2866 4545 XmnI GAAnn'nnTTC Cuts at: 2620 4098 5540 2620 Size: 1478 1442 4491 Fragments arranged by size: 4491 1478 1442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4661 5219 276 Size: 53 83 186 936 3127 558 2468 Fragments arranged by size: 3127 2468 936 558 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI