(Linear) (Six-base) MAPSORT of: KIBB8544.seq Check: 8601 from: 1 to: 2706 KIBB8544.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AatII G_ACGT'C Cuts at: 0 2658 2706 Size: 2658 48 AccI GT'mk_AC Cuts at: 0 2278 2706 Size: 2278 428 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 926 1157 1196 1666 1870 2706 Size: 305 621 231 39 470 204 836 Fragments arranged by size: 836 621 470 305 231 204 39 AflIII A'CryG_T Cuts at: 0 2447 2449 2706 Size: 2447 2 257 Fragments arranged by size: 2447 257 2 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 776 808 2706 Size: 776 32 1898 Fragments arranged by size: 1898 776 32 AlwNI CAG_nnn'CTG Cuts at: 0 283 2706 Size: 283 2423 ApoI r'AATT_y Cuts at: 0 172 662 1543 1754 2388 2465 2562 2706 Size: 172 490 881 211 634 77 97 144 Fragments arranged by size: 881 634 490 211 172 144 97 77 AvaI C'yCGr_G Cuts at: 0 9 968 2706 Size: 9 959 1738 Fragments arranged by size: 1738 959 9 AvrII C'CTAG_G Cuts at: 0 1245 2706 Size: 1245 1461 BanII G_rGCy'C Cuts at: 0 829 845 1307 1658 2706 Size: 829 16 462 351 1048 Fragments arranged by size: 1048 829 462 351 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 476 617 1582 2706 Size: 476 141 965 1124 Fragments arranged by size: 1124 965 476 141 BbvCI CC'TCA_GC Cuts at: 0 196 2706 Size: 196 2510 BciVI GTATCCnnnnn_n' Cuts at: 0 1953 2706 Size: 1953 753 BclI T'GATC_A Cuts at: 0 432 2706 Size: 432 2274 BfrBI ATG'CAT Cuts at: 0 1673 2706 Size: 1673 1033 BglII A'GATC_T Cuts at: 0 2625 2706 Size: 2625 81 BlpI GC'TnA_GC Cuts at: 0 452 2706 Size: 452 2254 Bme1580I G_kGCm'C Cuts at: 0 1149 2447 2706 Size: 1149 1298 259 Fragments arranged by size: 1298 1149 259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 1688 2706 Size: 397 1291 1018 Fragments arranged by size: 1291 1018 397 Bpu10I CC'TnA_GC Cuts at: 0 196 2706 Size: 196 2510 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 797 935 1584 2505 2706 Size: 797 138 649 921 201 Fragments arranged by size: 921 797 649 201 138 BsaAI yAC'GTr Cuts at: 0 1771 2450 2706 Size: 1771 679 256 Fragments arranged by size: 1771 679 256 BsaBI GATnn'nnATC Cuts at: 0 1131 1192 2630 2706 Size: 1131 61 1438 76 Fragments arranged by size: 1438 1131 76 61 BsaHI Gr'CG_yC Cuts at: 0 2655 2706 Size: 2655 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1439 1469 2045 2075 2230 2260 2290 2706 Size: 1439 30 576 30 155 30 30 416 Fragments arranged by size: 1439 576 416 155 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 935 938 2066 2281 2437 2706 Size: 935 3 1128 215 156 269 Fragments arranged by size: 1128 935 269 215 156 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 1611 2706 Size: 296 1315 1095 Fragments arranged by size: 1315 1095 296 BsiHKAI G_wGCw'C Cuts at: 0 587 845 2706 Size: 587 258 1861 Fragments arranged by size: 1861 587 258 BsmI GAATG_Cn' Cuts at: 0 1675 2706 Size: 1675 1031 Bsp1286I G_dGCh'C Cuts at: 0 587 829 845 1149 1307 1658 2447 2706 Size: 587 242 16 304 158 351 789 259 Fragments arranged by size: 789 587 351 304 259 242 158 16 BspHI T'CATG_A Cuts at: 0 90 219 1824 2706 Size: 90 129 1605 882 Fragments arranged by size: 1605 882 129 90 BsrFI r'CCGG_y Cuts at: 0 521 2706 Size: 521 2185 BsrGI T'GTAC_A Cuts at: 0 2074 2706 Size: 2074 632 BstYI r'GATC_y Cuts at: 0 1473 1971 2625 2706 Size: 1473 498 654 81 Fragments arranged by size: 1473 654 498 81 BtgI C'CryG_G Cuts at: 0 1857 2706 Size: 1857 849 ClaI AT'CG_AT Cuts at: 0 1485 2706 Size: 1485 1221 DraI TTT'AAA Cuts at: 0 1752 2358 2706 Size: 1752 606 348 Fragments arranged by size: 1752 606 348 DraIII CAC_nnn'GTG Cuts at: 0 591 2706 Size: 591 2115 EaeI y'GGCC_r Cuts at: 0 704 2706 Size: 704 2002 EarI CTCTTCn'nnn_ Cuts at: 0 2210 2258 2706 Size: 2210 48 448 Fragments arranged by size: 2210 448 48 EcoICRI GAG'CTC Cuts at: 0 843 2706 Size: 843 1863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 397 926 1157 1196 1666 1688 1870 Size: 305 92 529 231 39 470 22 182 Cuts at: 1870 2706 Size: 836 Fragments arranged by size: 836 529 470 305 231 182 92 39 22 EcoO109I rG'GnC_Cy Cuts at: 0 63 817 2706 Size: 63 754 1889 Fragments arranged by size: 1889 754 63 EcoRI G'AATT_C Cuts at: 0 172 2706 Size: 172 2534 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1547 1576 2706 Size: 1547 29 1130 Fragments arranged by size: 1547 1130 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 933 965 1956 1988 2209 2230 Size: 730 32 171 32 991 32 221 21 Cuts at: 2230 2241 2260 2262 2292 2706 Size: 11 19 2 30 414 Fragments arranged by size: 991 730 414 221 171 32 32 32 30 21 19 11 2 HincII GTy'rAC Cuts at: 0 519 2706 Size: 519 2187 HindIII A'AGCT_T Cuts at: 0 397 1385 2706 Size: 397 988 1321 Fragments arranged by size: 1321 988 397 HpaI GTT'AAC Cuts at: 0 519 2706 Size: 519 2187 MscI TGG'CCA Cuts at: 0 706 2706 Size: 706 2000 MslI CAynn'nnrTG Cuts at: 0 1668 2303 2706 Size: 1668 635 403 Fragments arranged by size: 1668 635 403 MspA1I CmG'CkG Cuts at: 0 20 195 451 2706 Size: 20 175 256 2255 Fragments arranged by size: 2255 256 175 20 NcoI C'CATG_G Cuts at: 0 1857 2706 Size: 1857 849 NdeI CA'TA_TG Cuts at: 0 293 2706 Size: 293 2413 NsiI A_TGCA'T Cuts at: 0 1675 2706 Size: 1675 1031 PasI CC'CwG_GG Cuts at: 0 1648 2706 Size: 1648 1058 PflMI CCAn_nnn'nTGG Cuts at: 0 855 2706 Size: 855 1851 PfoI T'CCnGG_A Cuts at: 0 1974 2706 Size: 1974 732 PmlI CAC'GTG Cuts at: 0 1771 2450 2706 Size: 1771 679 256 Fragments arranged by size: 1771 679 256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1803 1835 2706 Size: 1803 32 871 Fragments arranged by size: 1803 871 32 PpuMI rG'GwC_Cy Cuts at: 0 63 817 2706 Size: 63 754 1889 Fragments arranged by size: 1889 754 63 PshAI GACnn'nnGTC Cuts at: 0 689 2706 Size: 689 2017 PsiI TTA'TAA Cuts at: 0 1222 2706 Size: 1222 1484 PspXI vC'TCGA_Gb Cuts at: 0 968 2706 Size: 968 1738 PstI C_TGCA'G Cuts at: 0 42 2706 Size: 42 2664 PvuII CAG'CTG Cuts at: 0 20 195 451 2706 Size: 20 175 256 2255 Fragments arranged by size: 2255 256 175 20 SacI G_AGCT'C Cuts at: 0 845 2706 Size: 845 1861 SexAI A'CCwGG_T Cuts at: 0 1846 2706 Size: 1846 860 SfcI C'TryA_G Cuts at: 0 38 379 761 2706 Size: 38 341 382 1945 Fragments arranged by size: 1945 382 341 38 SmlI C'TyrA_G Cuts at: 0 812 950 968 1599 2520 2706 Size: 812 138 18 631 921 186 Fragments arranged by size: 921 812 631 186 138 18 SspI AAT'ATT Cuts at: 0 2675 2706 Size: 2675 31 StuI AGG'CCT Cuts at: 0 1612 2706 Size: 1612 1094 StyI C'CwwG_G Cuts at: 0 831 1245 1518 1857 2706 Size: 831 414 273 339 849 Fragments arranged by size: 849 831 414 339 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2562 2706 Size: 2562 144 TatI w'GTAC_w Cuts at: 0 102 2074 2706 Size: 102 1972 632 Fragments arranged by size: 1972 632 102 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1439 1471 2045 2077 2706 Size: 1439 32 574 32 629 Fragments arranged by size: 1439 629 574 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2323 2706 Size: 2323 383 XbaI T'CTAG_A Cuts at: 0 1108 2706 Size: 1108 1598 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2564 2706 Size: 2564 142 XhoI C'TCGA_G Cuts at: 0 968 2706 Size: 968 1738 XmnI GAAnn'nnTTC Cuts at: 0 615 2093 2706 Size: 615 1478 613 Fragments arranged by size: 1478 615 613 ZraI GAC'GTC Cuts at: 0 2656 2706 Size: 2656 50 Enzymes that do cut: AatII AccI AcuI AflIII AloI AlwNI ApoI AvaI AvrII BanII BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BsrGI BstYI BtgI ClaI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MscI MslI MspA1I NcoI NdeI NsiI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuII SacI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanI BbeI BcgI BglI BmgBI BmrI BmtI BplI BsaI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XmaI