################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MaeI MamI MluI MluNI MroI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 308 GACGTC GACGT^C AccII 925 CGCG CG^CG Alw44I 653 GTGCAC G^TGCAC ApaLI 653 GTGCAC G^TGCAC AspHI 653 GTGCAC G[AT]GC[AT]^C AvaII 964 GGTCC G^G[AT]CC BanI 1030 GGCGCC G^G[CT][GA]CC BbeI 1030 GGCGCC GGCGC^C BbsI 1266 GTCTTC GAAGAC(2/6) BbvCI 1024 GCTGAGG CCTCAGC(-5/-2) BcnI 25 CCCGG CC^[GC]GG BpmI 593 CTCCAG CTGGAG(16/14) BpuAI 1266 GTCTTC GAAGAC(2/6) BsiHKAI 653 GTGCAC G[AT]GC[AT]^C BsmAI 497 GAGAC GTCTC(1/5) BsrBI 910 GAGCGG CCGCTC(-3/-3) BsrDI 1273 GCAATG GCAATG(2/0) BsrI 1059 ACTGG ACTGG(1/-1) BstUI 925 CGCG CG^CG DraIII 647 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 726 GAAGAG CTCTTC(1/4) EcoNI 672 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1030 GGCGCC [GA]GCGC^[CT] HapII 26 CCGG C^CGG HpaII 26 CCGG C^CGG KasI 1030 GGCGCC G^GCGCC Ksp632I 726 GAAGAG CTCTTC(1/4) MfeI 1299 CAATTG C^AATTG MspI 26 CCGG C^CGG MunI 1299 CAATTG C^AATTG MvnI 925 CGCG CG^CG NarI 1030 GGCGCC GG^CGCC NciI 25 CCCGG CC^[GC]GG PpuMI 963 GGGTCCC [GA]G^G[AT]CC[CT] PstI 848 CTGCAG CTGCA^G ScaI 1391 AGTACT AGT^ACT SfoI 1030 GGCGCC GGC^GCC SspI 763 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 1422 GATCC GGATC(4/5) 1295 GGATC BglI 52 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 250 GCCCGCCTGGC BstAPI 69 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 352 GCAGTTCTTGC CfoI 924 GCGC GCG^C 1031 GCGC DraII 963 GGGTCCC [GA]G^G[ACGT]CC[CT] 1253 AGGCCCT DsaI 365 CCATGG C^C[GA][CT]GG 553 CCATGG EcoO109I 963 GGGTCCC [GA]G^G[ACGT]CC[CT] 1253 AGGCCCT HgaI 61 GCGTC GACGC(5/10) 425 GCGTC HhaI 924 GCGC GCG^C 1031 GCGC HinP1I 924 GCGC G^CGC 1031 GCGC HphI 33 GGTGA GGTGA(8/7) 1410 GGTGA MaeII 309 ACGT A^CGT 1103 ACGT MspA1I 707 CAGCTG C[AC]G^C[GT]G 1226 CAGCTG NcoI 365 CCATGG C^CATGG 553 CCATGG PvuII 707 CAGCTG CAG^CTG 1226 CAGCTG SfcI 488 CTGTAG C^T[GA][CT]AG 848 CTGCAG TfiI 370 GAATC G^A[AT]TC 1340 GATTC Tsp45I 1105 GTCAC ^GT[GC]AC 1411 GTGAC XcmI 699 CCAAGAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1323 CCAGAGCCCCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI ApoI BanII BbvI BglII BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeIII Hin1I HindIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII