(Circular) (Six-base) MAPSORT of: pFN21AE6415.seq Check: 3214 from: 1 to: 6145 pFN21AE6415 6145 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2317 3955 278 Size: 53 83 186 936 781 1638 2468 Fragments arranged by size: 2468 1638 936 781 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 4208 5592 5860 1632 Size: 654 1922 1384 268 1917 Fragments arranged by size: 1922 1917 1384 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 2820 1166 Size: 1654 4491 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaLI G'TGCA_C Cuts at: 2658 4202 5358 2658 Size: 1544 1156 3445 Fragments arranged by size: 3445 1544 1156 ApoI r'AATT_y Cuts at: 1493 2472 3162 3380 3454 3680 3832 5941 1493 Size: 979 690 218 74 226 152 2109 1697 Fragments arranged by size: 2109 1697 979 690 226 218 152 74 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 3470 1870 Size: 73 1527 4545 Fragments arranged by size: 4545 1527 73 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3035 3466 4921 618 Size: 325 217 627 1248 431 1455 1842 Fragments arranged by size: 1842 1455 1248 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2677 3093 3335 3464 1391 Size: 1286 416 242 129 4072 Fragments arranged by size: 4072 1286 416 242 129 BbeI G_GCGC'C Cuts at: 3039 3039 Size: 6145 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3264 928 Size: 971 1365 3809 Fragments arranged by size: 3809 1365 971 BbvCI CC'TCA_GC Cuts at: 3030 3030 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2261 4756 Size: 107 122 71 631 605 391 198 2495 Cuts at: 4756 136 Size: 1525 Fragments arranged by size: 2495 1525 631 605 391 198 122 107 71 BglII A'GATC_T Cuts at: 2121 3000 3141 6140 2121 Size: 879 141 2999 2126 Fragments arranged by size: 2999 2126 879 141 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2662 4206 5362 1790 Size: 872 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 872 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3074 3876 4834 Size: 416 53 83 399 24 1657 802 958 Cuts at: 4834 442 Size: 1753 Fragments arranged by size: 1753 1657 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2757 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2583 4805 1239 Size: 1344 2222 2579 Fragments arranged by size: 2579 2222 1344 Bpu10I CC'TnA_GC Cuts at: 3030 3030 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2314 2430 3036 Size: 53 83 186 936 533 248 116 606 Cuts at: 3036 3952 4334 4969 275 Size: 916 382 635 1451 Fragments arranged by size: 1451 936 916 635 606 533 382 248 186 116 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 1178 Size: 30 165 30 781 30 5109 Fragments arranged by size: 5109 781 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 2906 3152 1186 Size: 1705 15 246 4179 Fragments arranged by size: 4179 1705 246 15 BseYI C'CCAG_C Cuts at: 2460 3287 5348 5844 2460 Size: 827 2061 496 2761 Fragments arranged by size: 2761 2061 827 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2267 2833 1163 Size: 573 321 210 566 4475 Fragments arranged by size: 4475 573 566 321 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2662 4206 4291 5362 2662 Size: 1544 85 1071 3445 Fragments arranged by size: 3445 1544 1071 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2662 2677 3093 3335 3464 4206 4291 Size: 399 872 15 416 242 129 742 85 Cuts at: 4291 5362 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 872 742 416 399 242 129 85 15 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 844 Size: 2656 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2917 4032 1394 Size: 429 1094 1115 3507 Fragments arranged by size: 3507 1115 1094 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3285 4640 4814 65 Size: 1183 2037 1355 174 1396 Fragments arranged by size: 2037 1396 1355 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 2363 794 Size: 1286 283 4576 Fragments arranged by size: 4576 1286 283 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2121 3000 3141 3426 3475 3842 4227 4244 Size: 149 879 141 285 49 367 385 17 Cuts at: 4244 5696 5782 5794 6140 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 879 385 367 346 285 149 141 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 2558 513 Size: 552 1305 188 4100 Fragments arranged by size: 4100 1305 552 188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 2250 2850 3661 4455 4475 2250 Size: 600 811 794 20 3920 Fragments arranged by size: 3920 811 794 600 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DraIII CAC_nnn'GTG Cuts at: 2657 2657 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3592 4481 6110 Size: 54 1425 50 275 18 1762 889 1629 Cuts at: 6110 8 Size: 43 Fragments arranged by size: 1762 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 4075 1353 Size: 1373 1349 3423 Fragments arranged by size: 3423 1373 1349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2583 4208 4805 5592 5860 1239 Size: 393 654 297 1625 597 787 268 1524 Fragments arranged by size: 1625 1524 787 654 597 393 297 268 EcoNI CCTnn'n_nnAGG Cuts at: 2681 2681 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2969 3259 2969 Size: 290 5855 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 3039 5292 1678 Size: 1361 2253 2531 Fragments arranged by size: 2531 2253 1361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 2664 2696 4786 Size: 32 135 32 966 32 94 32 2090 Cuts at: 4786 4818 4860 4892 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2090 966 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 2327 2406 2696 748 Size: 1579 79 290 4197 Fragments arranged by size: 4197 1579 290 79 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KasI G'GCGC_C Cuts at: 3035 3035 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3304 3744 3304 Size: 440 5705 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2671 3129 5259 5443 1305 Size: 996 370 458 2130 184 2007 Fragments arranged by size: 2130 2007 996 458 370 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 2820 2870 4103 4462 4621 518 Size: 648 1371 283 50 1233 359 159 2042 Fragments arranged by size: 2042 1371 1233 648 359 283 159 50 MspA1I CmG'CkG Cuts at: 1606 2714 3233 3561 4238 5386 5631 1606 Size: 1108 519 328 677 1148 245 2120 Fragments arranged by size: 2120 1148 1108 677 519 328 245 NarI GG'CG_CC Cuts at: 3036 3036 Size: 6145 NcoI C'CATG_G Cuts at: 513 1065 2370 2558 513 Size: 552 1305 188 4100 Fragments arranged by size: 4100 1305 552 188 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3503 5048 1132 Size: 97 2274 1545 2229 Fragments arranged by size: 2274 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2969 2969 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 2129 3715 2129 Size: 1586 4559 PstI C_TGCA'G Cuts at: 830 2857 3497 830 Size: 2027 640 3478 Fragments arranged by size: 3478 2027 640 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2714 3233 2714 Size: 519 5626 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SanDI GG'GwC_CC Cuts at: 2969 2969 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 3398 4393 6105 3398 Size: 995 1712 3438 Fragments arranged by size: 3438 1712 995 SfcI C'TryA_G Cuts at: 826 1045 2493 2853 3493 4628 5309 5500 826 Size: 219 1448 360 640 1135 681 191 1471 Fragments arranged by size: 1471 1448 1135 681 640 360 219 191 SfoI GGC'GCC Cuts at: 3037 3037 Size: 6145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 2770 4069 5 Size: 47 2718 1299 2081 Fragments arranged by size: 2718 2081 1299 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2370 2558 3132 3584 513 Size: 552 1305 188 574 452 3074 Fragments arranged by size: 3074 1305 574 552 452 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4172 4189 4342 4527 4972 325 Size: 1255 2592 17 153 185 445 1498 Fragments arranged by size: 2592 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 2991 3396 Size: 274 80 33 51 1443 135 879 405 Cuts at: 3396 4391 6103 96 Size: 995 1712 138 Fragments arranged by size: 1712 1443 995 879 405 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 6105 6137 2184 Size: 32 3889 32 2192 Fragments arranged by size: 3889 2192 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2711 3335 2711 Size: 624 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2315 3953 276 Size: 53 83 186 936 781 1638 2468 Fragments arranged by size: 2468 1638 936 781 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI