Restriction map for
pFN21AB9983
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccII AclI AflII
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
HgaI HindIII HpaI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NcoI NdeI NgoMIV NotI
NruI NspV PacI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
Acc65I 844 GGTACC G^GTACC
AccIII 1086 TCCGGA T^CCGGA
AcyI 1477 GGCGCC G[GA]^CG[CT]C
AflIII 1471 ACATGT A^C[GA][CT]GT
AgeI 784 ACCGGT A^CCGGT
Alw44I 1510 GTGCAC G^TGCAC
AlwI 216 GGATC GGATC(4/5)
AocI 1515 CCTGAGG CC^T[ACGT]AGG
ApaLI 1510 GTGCAC G^TGCAC
Asp718I 844 GGTACC G^GTACC
AspI 1166 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 464 CTCGAG C^[CT]CG[GA]G
BbeI 1477 GGCGCC GGCGC^C
BbsI 1005 GTCTTC GAAGAC(2/6)
BciVI 1119 GTATCC GTATCC(6/5)
BclI 945 TGATCA T^GATCA
BpuAI 1005 GTCTTC GAAGAC(2/6)
BsaHI 1477 GGCGCC G[GA]^CG[CT]C
BseAI 1086 TCCGGA T^CCGGA
BsgI 498 GTGCAG GTGCAG(16/14)
BsoBI 464 CTCGAG C^[CT]CG[GA]G
BspDI 1100 ATCGAT AT^CGAT
BspEI 1086 TCCGGA T^CCGGA
BspHI 368 TCATGA T^CATGA
BspLU11I 1471 ACATGT A^CATGT
BsrDI 1137 CATTGC GCAATG(2/0)
BsrFI 784 ACCGGT [GA]^CCGG[CT]
BstAPI 1245 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 216 GGATCT [GA]^GATC[CT]
Bsu36I 1515 CCTGAGG CC^T[ACGT]AGG
Cfr10I 784 ACCGGT [GA]^CCGG[CT]
ClaI 1100 ATCGAT AT^CGAT
DraII 983 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 1515 CCTGAGG CC^T[ACGT]AGG
EcoO109I 983 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 624 GAATTC G^AATTC
EcoRV 94 GATATC GAT^ATC
EcoT14I 685 CCTTGG C^C[AT][AT]GG
EcoT22I 1330 ATGCAT ATGCA^T
FauI 1211 GCGGG CCCGC(4/6)
FbaI 945 TGATCA T^GATCA
HaeII 1477 GGCGCC [GA]GCGC^[CT]
Hin1I 1477 GGCGCC G[GA]^CG[CT]C
HincII 664 GTTGAC GT[CT]^[GA]AC
HindII 664 GTTGAC GT[CT]^[GA]AC
KasI 1477 GGCGCC G^GCGCC
KpnI 844 GGTACC GGTAC^C
MaeII 1066 ACGT A^CGT
MflI 216 GGATCT [GA]^GATC[CT]
MroI 1086 TCCGGA T^CCGGA
MspA1I 1290 CCGCTG C[AC]G^C[GT]G
NarI 1477 GGCGCC GG^CGCC
NheI 1545 GCTAGC G^CTAGC
NsiI 1330 ATGCAT ATGCA^T
PaeR7I 464 CTCGAG C^TCGAG
PflFI 1166 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 784 ACCGGT A^CCGGT
PpuMI 983 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 368 TCATGA T^CATGA
SfcI 1013 CTATAG C^T[GA][CT]AG
SfoI 1477 GGCGCC GGC^GCC
SmlI 464 CTCGAG C^T[CT][GA]AG
SspI 298 AATATT AAT^ATT
StyI 685 CCTTGG C^C[AT][AT]GG
Tth111I 1166 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 420 TCTAGA T^CTAGA
XcmI 42 CCAGGAAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 464 CTCGAG C^TCGAG
XhoII 216 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 624 GAATTC [GA]^AATT[CT]
1500 AAATTT
AlwNI 233 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
351 CAGCACCTG
ApoI 624 GAATTC [GA]^AATT[CT]
1500 AAATTT
Asp700I 1108 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1299 GAAAAAGTTC
AspHI 819 GAGCAC G[AT]GC[AT]^C
1510 GTGCAC
BsaBI 217 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1093 GATGATGATC
BsaI 781 GAGACC GGTCTC(1/5)
1609 GAGACC
BsiHKAI 819 GAGCAC G[AT]GC[AT]^C
1510 GTGCAC
BsmFI 909 GTCCC GGGAC(10/14)
1181 GTCCC
BspMI 355 ACCTGC ACCTGC(4/8)
1207 ACCTGC
BsrI 238 ACTGG ACTGG(1/-1)
661 CCAGT
CfoI 549 GCGC GCG^C
1478 GCGC
HaeIII 184 GGCC GG^CC
772 GGCC
HhaI 549 GCGC GCG^C
1478 GCGC
HinP1I 549 GCGC G^CGC
1478 GCGC
MamI 217 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1093 GATGATGATC
NspI 1250 ACATGC [GA]CATG^[CT]
1471 ACATGT
PleI 667 GACTC GAGTC(4/5)
1521 GAGTC
XmnI 1108 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1299 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BfaI BmyI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I