(Circular) (Six-base) MAPSORT of: pFN21AB9983.seq Check: 7037 from: 1 to: 6415 pFN21AB9983 6415 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2369 2369 Size: 6415 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 2849 3736 2849 Size: 887 5528 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2619 4478 5862 6130 1632 Size: 987 1859 1384 268 1917 Fragments arranged by size: 1917 1859 1384 987 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 3476 5314 6291 1203 Size: 2273 1838 977 1327 Fragments arranged by size: 2273 1838 1327 977 AgeI A'CCGG_T Cuts at: 2789 5246 2789 Size: 2457 3958 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6415 AlwNI CAG_nnn'CTG Cuts at: 2243 2361 4158 5730 2243 Size: 118 1797 1572 2928 Fragments arranged by size: 2928 1797 1572 118 ApaLI G'TGCA_C Cuts at: 3515 4472 5628 3515 Size: 957 1156 4302 Fragments arranged by size: 4302 1156 957 ApoI r'AATT_y Cuts at: 1493 2629 3505 3724 3950 4102 6211 1493 Size: 1136 876 219 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 876 226 219 152 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 2469 3740 1870 Size: 73 526 1271 4545 Fragments arranged by size: 4545 1271 526 73 BamHI G'GATC_C Cuts at: 3745 4112 3745 Size: 367 6048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2849 3372 3482 3736 5191 Size: 325 217 627 1062 523 110 254 1455 Cuts at: 5191 618 Size: 1842 Fragments arranged by size: 1842 1455 1062 627 523 325 254 217 110 BanII G_rGCy'C Cuts at: 1391 3734 1391 Size: 2343 4072 BbeI G_GCGC'C Cuts at: 3486 3486 Size: 6415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3003 928 Size: 971 1104 4340 Fragments arranged by size: 4340 1104 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3135 4307 5517 3135 Size: 1172 1210 4033 Fragments arranged by size: 4033 1210 1172 BclI T'GATC_A Cuts at: 1548 2950 1548 Size: 1402 5013 BfrBI ATG'CAT Cuts at: 3337 3337 Size: 6415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5026 136 Size: 107 122 71 631 605 3354 1525 Fragments arranged by size: 3354 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 2137 3519 4476 5632 1790 Size: 347 1382 957 1156 2573 Fragments arranged by size: 2573 1382 1156 957 347 BmgBI CAC'GTC Cuts at: 1318 3072 1318 Size: 1754 4661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4146 5104 442 Size: 416 53 83 399 24 2729 958 1753 Fragments arranged by size: 2729 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3554 1056 Size: 2498 3917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2502 2502 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5075 1239 Size: 3836 2579 Bpu10I CC'TnA_GC Cuts at: 2680 2680 Size: 6415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4540 5405 5703 4540 Size: 865 298 5252 Fragments arranged by size: 5252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 3608 5078 882 Size: 617 1281 828 1470 2219 Fragments arranged by size: 2219 1470 1281 828 617 BsaAI yAC'GTr Cuts at: 493 1204 4206 493 Size: 711 3002 2702 Fragments arranged by size: 3002 2702 711 BsaBI GATnn'nnATC Cuts at: 2226 3102 6409 2226 Size: 876 3307 2232 Fragments arranged by size: 3307 2232 876 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3483 4222 4604 5239 Size: 53 83 186 936 1950 739 382 635 Cuts at: 5239 275 Size: 1451 Fragments arranged by size: 1950 1451 936 739 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2789 3091 3369 3586 4848 5246 5520 5667 6078 Size: 302 278 217 1262 398 274 147 411 Cuts at: 6078 2789 Size: 3126 Fragments arranged by size: 3126 1262 411 398 302 278 274 217 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3070 3100 1178 Size: 30 165 30 1667 30 4493 Fragments arranged by size: 4493 1667 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2909 3078 3538 3652 1186 Size: 837 886 169 460 114 3949 Fragments arranged by size: 3949 886 837 460 169 114 BseYI C'CCAG_C Cuts at: 2916 5618 6114 2916 Size: 2702 496 3217 Fragments arranged by size: 3217 2702 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2524 1163 Size: 573 788 5054 Fragments arranged by size: 5054 788 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3865 4626 5654 1540 Size: 275 186 1864 761 1028 2301 Fragments arranged by size: 2301 1864 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2828 3519 4476 4561 5632 2828 Size: 691 957 85 1071 3611 Fragments arranged by size: 3611 1071 957 691 85 BsmI GAATG_Cn' Cuts at: 3926 4019 3926 Size: 93 6322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2828 3519 3734 4476 4561 5632 Size: 399 347 691 691 215 742 85 1071 Cuts at: 5632 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 691 691 399 347 215 85 BspEI T'CCGG_A Cuts at: 3091 6078 3091 Size: 2987 3428 BspHI T'CATG_A Cuts at: 2373 4304 6034 2373 Size: 1931 1730 2754 Fragments arranged by size: 2754 1931 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2369 3221 3770 844 Size: 1525 852 549 3489 Fragments arranged by size: 3489 1525 852 549 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3141 4910 5084 65 Size: 1183 1893 1769 174 1396 Fragments arranged by size: 1893 1769 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2789 5059 5246 1700 Size: 110 979 2270 187 2869 Fragments arranged by size: 2869 2270 979 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 4475 5487 4475 Size: 1012 5403 BstAPI GCAn_nnn'nTGC Cuts at: 794 3256 794 Size: 2462 3953 BstBI TT'CG_AA Cuts at: 5219 5219 Size: 6415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstYI r'GATC_y Cuts at: 1972 2221 3745 4112 4497 4514 5966 6052 6064 Size: 249 1524 367 385 17 1452 86 12 Cuts at: 6064 6410 1972 Size: 346 1977 Fragments arranged by size: 1977 1524 1452 385 367 346 249 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3521 3521 Size: 6415 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4924 Fragments arranged by size: 4924 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3323 487 Size: 683 56 2097 3579 Fragments arranged by size: 3579 2097 683 56 BtsI GCAGTG_nn' Cuts at: 2167 2512 3577 3931 4725 4745 2167 Size: 345 1065 354 794 20 3837 Fragments arranged by size: 3837 1065 794 354 345 20 ClaI AT'CG_AT Cuts at: 1554 3106 1554 Size: 1552 4863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3460 3495 529 Size: 35 2896 35 3449 Fragments arranged by size: 3449 2896 35 35 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3862 4751 6380 Size: 54 1425 50 275 18 2032 889 1629 Cuts at: 6380 8 Size: 43 Fragments arranged by size: 2032 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2041 2680 3606 4345 1353 Size: 688 639 926 739 3423 Fragments arranged by size: 3423 926 739 688 639 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5002 5376 5522 387 Size: 1064 138 3413 374 146 1280 Fragments arranged by size: 3413 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2619 4478 5075 5862 6130 1239 Size: 393 987 1859 597 787 268 1524 Fragments arranged by size: 1859 1524 987 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2989 2989 Size: 6415 EcoRI G'AATT_C Cuts at: 2629 3724 4102 2629 Size: 1095 378 4942 Fragments arranged by size: 4942 1095 378 EcoRV GAT'ATC Cuts at: 2101 2101 Size: 6415 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3008 3635 3008 Size: 627 5788 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 3486 5562 1678 Size: 1808 2076 2531 Fragments arranged by size: 2531 2076 1808 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 5056 5088 5130 Size: 32 135 32 1325 32 2127 32 42 Cuts at: 5130 5162 1373 Size: 32 2626 Fragments arranged by size: 2626 2127 1325 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2671 3759 4005 669 Size: 988 281 733 1088 246 3079 Fragments arranged by size: 3079 1088 988 733 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KasI G'GCGC_C Cuts at: 3482 3482 Size: 6415 KpnI G_GTAC'C Cuts at: 2853 3740 2853 Size: 887 5528 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 3233 5529 5713 1305 Size: 894 1034 2296 184 2007 Fragments arranged by size: 2296 2007 1034 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4593 Fragments arranged by size: 4593 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2094 2570 3286 4373 4732 4891 Size: 648 843 85 476 716 1087 359 159 Cuts at: 4891 518 Size: 2042 Fragments arranged by size: 2042 1087 843 716 648 476 359 159 85 MspA1I CmG'CkG Cuts at: 1606 3297 3831 4508 5656 5901 1606 Size: 1691 534 677 1148 245 2120 Fragments arranged by size: 2120 1691 1148 677 534 245 NarI GG'CG_CC Cuts at: 3483 3483 Size: 6415 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4924 Fragments arranged by size: 4924 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NheI G'CTAG_C Cuts at: 1052 3550 1052 Size: 2498 3917 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NsiI A_TGCA'T Cuts at: 3339 3339 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 3259 3480 3773 5318 1132 Size: 97 2030 221 293 1545 2229 Fragments arranged by size: 2229 2030 1545 293 221 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6415 PciI A'CATG_T Cuts at: 3476 5314 3476 Size: 1838 4577 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6415 PfoI T'CCnGG_A Cuts at: 2115 2543 2965 2115 Size: 428 422 5565 Fragments arranged by size: 5565 428 422 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4484 4516 5997 6029 4484 Size: 32 1481 32 4870 Fragments arranged by size: 4870 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2989 2989 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 3985 3985 Size: 6415 PstI C_TGCA'G Cuts at: 830 3767 830 Size: 2937 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SfcI C'TryA_G Cuts at: 826 1045 3018 3763 4898 5579 5770 826 Size: 219 1973 745 1135 681 191 1471 Fragments arranged by size: 1973 1471 1135 745 681 219 191 SfoI GGC'GCC Cuts at: 3484 3484 Size: 6415 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2469 4519 5420 5682 820 Size: 197 926 526 2050 901 262 1553 Fragments arranged by size: 2050 1553 926 901 526 262 197 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 3773 1132 Size: 2641 3774 SspI AAT'ATT Cuts at: 5 52 2305 4339 5 Size: 47 2253 2034 2081 Fragments arranged by size: 2253 2081 2034 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2004 2690 3854 513 Size: 552 939 686 1164 3074 Fragments arranged by size: 3074 1164 939 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2416 4442 4459 4612 4797 5242 Size: 1255 417 419 2026 17 153 185 445 Cuts at: 5242 325 Size: 1498 Fragments arranged by size: 2026 1498 1255 445 419 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3161 4661 6373 Size: 274 80 33 51 1443 1184 1500 1712 Cuts at: 6373 96 Size: 138 Fragments arranged by size: 1712 1500 1443 1184 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3070 3102 6375 6407 3070 Size: 32 3273 32 3078 Fragments arranged by size: 3273 3078 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3174 1316 Size: 1858 4557 XbaI T'CTAG_A Cuts at: 2425 3751 2425 Size: 1326 5089 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2054 2054 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 2469 1943 Size: 526 5889 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 3117 3308 4544 3117 Size: 191 1236 4988 Fragments arranged by size: 4988 1236 191 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI