Restriction map for
pFN21AB9983
(Linear) (Six-base) MAPSORT of: KIBB9983.seq Check: 8939 from: 1 to: 1710
KIBB9983.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 364 1710
Size: 364 1346
Acc65I G'GTAC_C
Cuts at: 0 844 1710
Size: 844 866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1710
Size: 614 1096
AflIII A'CryG_T
Cuts at: 0 1471 1710
Size: 1471 239
AgeI A'CCGG_T
Cuts at: 0 784 1710
Size: 784 926
AlwNI CAG_nnn'CTG
Cuts at: 0 238 356 1710
Size: 238 118 1354
Fragments arranged by size:
1354 238 118
ApaLI G'TGCA_C
Cuts at: 0 1510 1710
Size: 1510 200
ApoI r'AATT_y
Cuts at: 0 624 1500 1710
Size: 624 876 210
Fragments arranged by size:
876 624 210
AvaI C'yCGr_G
Cuts at: 0 464 1710
Size: 464 1246
BanI G'GyrC_C
Cuts at: 0 844 1367 1477 1710
Size: 844 523 110 233
Fragments arranged by size:
844 523 233 110
BbeI G_GCGC'C
Cuts at: 0 1481 1710
Size: 1481 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 998 1710
Size: 998 712
BciVI GTATCCnnnnn_n'
Cuts at: 0 1130 1710
Size: 1130 580
BclI T'GATC_A
Cuts at: 0 945 1710
Size: 945 765
BfrBI ATG'CAT
Cuts at: 0 1332 1710
Size: 1332 378
Bme1580I G_kGCm'C
Cuts at: 0 132 1514 1710
Size: 132 1382 196
Fragments arranged by size:
1382 196 132
BmgBI CAC'GTC
Cuts at: 0 1067 1710
Size: 1067 643
BmtI G_CTAG'C
Cuts at: 0 1549 1710
Size: 1549 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 497 1710
Size: 497 1213
Bpu10I CC'TnA_GC
Cuts at: 0 675 1710
Size: 675 1035
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 1603 1710
Size: 775 828 107
Fragments arranged by size:
828 775 107
BsaBI GATnn'nnATC
Cuts at: 0 221 1097 1710
Size: 221 876 613
Fragments arranged by size:
876 613 221
BsaHI Gr'CG_yC
Cuts at: 0 1478 1710
Size: 1478 232
BsaWI w'CCGG_w
Cuts at: 0 784 1086 1364 1581 1710
Size: 784 302 278 217 129
Fragments arranged by size:
784 302 278 217 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1065 1095 1710
Size: 1065 30 615
Fragments arranged by size:
1065 615 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 904 1073 1533 1647 1710
Size: 18 886 169 460 114 63
Fragments arranged by size:
886 460 169 114 63 18
BseYI C'CCAG_C
Cuts at: 0 911 1710
Size: 911 799
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1710
Size: 519 1191
BsiHKAI G_wGCw'C
Cuts at: 0 823 1514 1710
Size: 823 691 196
Fragments arranged by size:
823 691 196
Bsp1286I G_dGCh'C
Cuts at: 0 132 823 1514 1710
Size: 132 691 691 196
Fragments arranged by size:
691 691 196 132
BspEI T'CCGG_A
Cuts at: 0 1086 1710
Size: 1086 624
BspHI T'CATG_A
Cuts at: 0 368 1710
Size: 368 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 364 1216 1710
Size: 364 852 494
Fragments arranged by size:
852 494 364
BsrDI GCAATG_nn'
Cuts at: 0 1136 1710
Size: 1136 574
BsrFI r'CCGG_y
Cuts at: 0 784 1710
Size: 784 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1251 1710
Size: 1251 459
BstYI r'GATC_y
Cuts at: 0 216 1710
Size: 216 1494
Bsu36I CC'TnA_GG
Cuts at: 0 1516 1710
Size: 1516 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1318 1710
Size: 1318 392
BtsI GCAGTG_nn'
Cuts at: 0 162 507 1572 1710
Size: 162 345 1065 138
Fragments arranged by size:
1065 345 162 138
ClaI AT'CG_AT
Cuts at: 0 1101 1710
Size: 1101 609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1455 1490 1710
Size: 1455 35 220
Fragments arranged by size:
1455 220 35
EarI CTCTTCn'nnn_
Cuts at: 0 36 675 1601 1710
Size: 36 639 926 109
Fragments arranged by size:
926 639 109 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1710
Size: 614 1096
EcoO109I rG'GnC_Cy
Cuts at: 0 984 1710
Size: 984 726
EcoRI G'AATT_C
Cuts at: 0 624 1710
Size: 624 1086
EcoRV GAT'ATC
Cuts at: 0 96 1710
Size: 96 1614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1003 1630 1710
Size: 1003 627 80
Fragments arranged by size:
1003 627 80
HaeII r_GCGC'y
Cuts at: 0 1481 1710
Size: 1481 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1710
Size: 892 32 786
Fragments arranged by size:
892 786 32
HincII GTy'rAC
Cuts at: 0 666 1710
Size: 666 1044
KasI G'GCGC_C
Cuts at: 0 1477 1710
Size: 1477 233
KpnI G_GTAC'C
Cuts at: 0 848 1710
Size: 848 862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1228 1710
Size: 194 1034 482
Fragments arranged by size:
1034 482 194
MslI CAynn'nnrTG
Cuts at: 0 89 565 1281 1710
Size: 89 476 716 429
Fragments arranged by size:
716 476 429 89
MspA1I CmG'CkG
Cuts at: 0 1292 1710
Size: 1292 418
NarI GG'CG_CC
Cuts at: 0 1478 1710
Size: 1478 232
NheI G'CTAG_C
Cuts at: 0 1545 1710
Size: 1545 165
NsiI A_TGCA'T
Cuts at: 0 1334 1710
Size: 1334 376
NspI r_CATG'y
Cuts at: 0 1254 1475 1710
Size: 1254 221 235
Fragments arranged by size:
1254 235 221
PciI A'CATG_T
Cuts at: 0 1471 1710
Size: 1471 239
PfoI T'CCnGG_A
Cuts at: 0 110 538 960 1710
Size: 110 428 422 750
Fragments arranged by size:
750 428 422 110
PpuMI rG'GwC_Cy
Cuts at: 0 984 1710
Size: 984 726
SfcI C'TryA_G
Cuts at: 0 1013 1710
Size: 1013 697
SfoI GGC'GCC
Cuts at: 0 1479 1710
Size: 1479 231
SmlI C'TyrA_G
Cuts at: 0 464 1710
Size: 464 1246
SspI AAT'ATT
Cuts at: 0 300 1710
Size: 300 1410
StyI C'CwwG_G
Cuts at: 0 685 1710
Size: 685 1025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 411 1710
Size: 411 1299
TatI w'GTAC_w
Cuts at: 0 1156 1710
Size: 1156 554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1065 1097 1710
Size: 1065 32 613
Fragments arranged by size:
1065 613 32
Tth111I GACn'n_nGTC
Cuts at: 0 1169 1710
Size: 1169 541
XbaI T'CTAG_A
Cuts at: 0 420 1710
Size: 420 1290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 49 1710
Size: 49 1661
XhoI C'TCGA_G
Cuts at: 0 464 1710
Size: 464 1246
XmnI GAAnn'nnTTC
Cuts at: 0 1112 1303 1710
Size: 1112 191 407
Fragments arranged by size:
1112 407 191
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AlwNI ApaLI ApoI
AvaI BanI BbeI BbsI BciVI BclI BfrBI Bme1580I
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BstAPI BstYI Bsu36I BtgZI BtsI
ClaI CspCI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I
NarI NheI NsiI NspI PciI PfoI PpuMI SfcI
SfoI SmlI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbvCI BcgI BglI BglII BlpI BmrI BpmI
BpuEI BsaAI BsiEI BsiWI BsmI BsmBI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI FseI
FspI FspAI HindIII HpaI MfeI MluI MscI NaeI
NcoI NdeI NgoMIV NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI XmaI ZraI