(Circular) (Six-base) MAPSORT of: pFN21AB8486.seq Check: 5743 from: 1 to: 7270 pFN21AB8486 7270 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3200 4337 3200 Size: 1137 6133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5080 278 Size: 53 83 186 936 3544 2468 Fragments arranged by size: 3544 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4613 1937 Size: 2676 4594 Acc65I G'GTAC_C Cuts at: 4591 4591 Size: 7270 AclI AA'CG_TT Cuts at: 1563 5397 5770 1563 Size: 3834 373 3063 Fragments arranged by size: 3834 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2425 2588 3577 5333 6717 6985 1632 Size: 764 29 163 989 1756 1384 268 1917 Fragments arranged by size: 1917 1756 1384 989 764 268 163 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7073 AflIII A'CryG_T Cuts at: 1203 6169 7146 1203 Size: 4966 977 1327 Fragments arranged by size: 4966 1327 977 AgeI A'CCGG_T Cuts at: 6101 6101 Size: 7270 AhdI GACnn_n'nnGTC Cuts at: 2800 5999 2800 Size: 3199 4071 AleI CACnn'nnGTG Cuts at: 1166 2588 1166 Size: 1422 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7270 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2315 Size: 32 7238 AlwNI CAG_nnn'CTG Cuts at: 2497 5013 6585 2497 Size: 2516 1572 3182 Fragments arranged by size: 3182 2516 1572 ApaI G_GGCC'C Cuts at: 2023 2042 2411 2746 2023 Size: 19 369 335 6547 Fragments arranged by size: 6547 369 335 19 ApaLI G'TGCA_C Cuts at: 5327 6483 5327 Size: 1156 6114 ApoI r'AATT_y Cuts at: 1493 2302 3152 4579 4805 4957 7066 1493 Size: 809 850 1427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 850 809 226 152 AseI AT'TA_AT Cuts at: 160 1032 5824 160 Size: 872 4792 1606 Fragments arranged by size: 4792 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 AvaI C'yCGr_G Cuts at: 1870 1943 2158 3364 4595 1870 Size: 73 215 1206 1231 4545 Fragments arranged by size: 4545 1231 1206 215 73 AvrII C'CTAG_G Cuts at: 3008 3008 Size: 7270 BamHI G'GATC_C Cuts at: 4600 4967 4600 Size: 367 6903 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2888 3118 3188 4591 6046 Size: 325 217 627 1101 230 70 1403 1455 Cuts at: 6046 618 Size: 1842 Fragments arranged by size: 1842 1455 1403 1101 627 325 230 217 70 BanII G_rGCy'C Cuts at: 1391 2023 2042 2411 2746 2855 2865 3174 3456 Size: 632 19 369 335 109 10 309 282 Cuts at: 3456 3738 4589 1391 Size: 282 851 4072 Fragments arranged by size: 4072 851 632 369 335 309 282 282 109 19 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 4210 928 Size: 971 364 1947 3988 Fragments arranged by size: 3988 1947 971 364 BbvCI CC'TCA_GC Cuts at: 4385 4385 Size: 7270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2440 2474 5461 5495 1903 Size: 34 503 34 2987 34 3678 Fragments arranged by size: 3678 2987 503 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 5162 6372 3407 Size: 1755 1210 4305 Fragments arranged by size: 4305 1755 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7270 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4232 5881 136 Size: 107 122 71 631 605 2560 1649 1525 Fragments arranged by size: 2560 1649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7265 7265 Size: 7270 BlpI GC'TnA_GC Cuts at: 2771 4687 2771 Size: 1916 5354 Bme1580I G_kGCm'C Cuts at: 1790 2023 2042 2411 2746 3123 3191 3860 5331 Size: 233 19 369 335 377 68 669 1471 Cuts at: 5331 6487 1790 Size: 1156 2573 Fragments arranged by size: 2573 1471 1156 669 377 369 335 233 68 19 BmgBI CAC'GTC Cuts at: 1318 2254 1318 Size: 936 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 4258 4572 Size: 416 53 83 399 24 1824 1017 314 Cuts at: 4572 5001 5959 442 Size: 429 958 1753 Fragments arranged by size: 1824 1753 1017 958 429 416 399 314 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7270 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3858 4192 3858 Size: 334 6936 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3188 3334 4169 4175 5930 1239 Size: 1949 146 835 6 1755 2579 Fragments arranged by size: 2579 1949 1755 835 146 6 Bpu10I CC'TnA_GC Cuts at: 2849 4385 2849 Size: 1536 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 3763 4070 5395 6260 6558 2048 Size: 1715 307 1325 865 298 2760 Fragments arranged by size: 2760 1715 1325 865 307 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3964 5933 882 Size: 617 2465 1969 2219 Fragments arranged by size: 2465 2219 1969 617 BsaAI yAC'GTr Cuts at: 493 1204 3038 3208 3378 3489 5061 493 Size: 711 1834 170 170 111 1572 2702 Fragments arranged by size: 2702 1834 1572 711 170 170 111 BsaBI GATnn'nnATC Cuts at: 7264 7264 Size: 7270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 3471 4206 5077 Size: 53 83 186 936 1224 714 735 871 Cuts at: 5077 5459 6094 275 Size: 382 635 1451 Fragments arranged by size: 1451 1224 936 871 735 714 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3399 5703 6101 6375 6522 6933 3399 Size: 2304 398 274 147 411 3736 Fragments arranged by size: 3736 2304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2315 2339 2345 2881 Size: 30 165 30 906 6 24 6 536 Cuts at: 2881 2885 2911 2915 3659 3689 1178 Size: 4 26 4 744 30 4759 Fragments arranged by size: 4759 906 744 536 165 30 30 30 26 24 6 6 4 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2519 2543 2914 1186 Size: 1003 330 24 371 5542 Fragments arranged by size: 5542 1003 371 330 24 BseYI C'CCAG_C Cuts at: 2166 2212 3423 4251 4479 6473 6969 2166 Size: 46 1211 828 228 1994 496 2467 Fragments arranged by size: 2467 1994 1211 828 496 228 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 2897 3085 3418 3592 4091 1163 Size: 573 906 255 188 333 174 499 4342 Fragments arranged by size: 4342 906 573 499 333 255 188 174 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2017 4720 5481 6509 1540 Size: 275 186 16 2703 761 1028 2301 Fragments arranged by size: 2703 2301 1028 761 275 186 16 BsiHKAI G_wGCw'C Cuts at: 3456 5331 5416 6487 3456 Size: 1875 85 1071 4239 Fragments arranged by size: 4239 1875 1071 85 BsmI GAATG_Cn' Cuts at: 4781 4874 4781 Size: 93 7177 BsmBI CGTCTCn'nnnn_ Cuts at: 4479 4479 Size: 7270 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2042 2411 2746 2855 2865 3123 Size: 399 233 19 369 335 109 10 258 Cuts at: 3123 3174 3191 3456 3738 3860 4589 5331 5416 Size: 51 17 265 282 122 729 742 85 Cuts at: 5416 6487 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 729 399 369 335 282 265 258 233 122 109 85 51 19 17 10 BspEI T'CCGG_A Cuts at: 3399 6933 3399 Size: 3534 3736 BspHI T'CATG_A Cuts at: 5159 6889 5159 Size: 1730 5540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3171 3200 3880 4337 4625 844 Size: 2327 29 680 457 288 3489 Fragments arranged by size: 3489 2327 680 457 288 29 BsrBI CCG'CTC Cuts at: 1394 1823 2723 2968 5157 1394 Size: 429 900 245 2189 3507 Fragments arranged by size: 3507 2189 900 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 3643 5765 5939 65 Size: 1183 2395 2122 174 1396 Fragments arranged by size: 2395 2122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3317 3473 4162 4226 4466 5914 6101 Size: 110 1507 156 689 64 240 1448 187 Cuts at: 6101 1700 Size: 2869 Fragments arranged by size: 2869 1507 1448 689 240 187 156 110 64 BsrGI T'GTAC_A Cuts at: 96 2385 2838 4518 96 Size: 2289 453 1680 2848 Fragments arranged by size: 2848 2289 1680 453 BssHII G'CGCG_C Cuts at: 1925 2558 4281 1925 Size: 633 1723 4914 Fragments arranged by size: 4914 1723 633 BssSI C'ACGA_G Cuts at: 2081 3832 3843 5330 6342 2081 Size: 1751 11 1487 1012 3009 Fragments arranged by size: 3009 1751 1487 1012 11 BstAPI GCAn_nnn'nTGC Cuts at: 794 4371 794 Size: 3577 3693 BstBI TT'CG_AA Cuts at: 6074 6074 Size: 7270 BstEII G'GTnAC_C Cuts at: 1184 2581 1184 Size: 1397 5873 BstXI CCAn_nnnn'nTGG Cuts at: 3955 3955 Size: 7270 BstYI r'GATC_y Cuts at: 1972 4305 4455 4600 4967 5352 5369 6821 6907 Size: 2333 150 145 367 385 17 1452 86 Cuts at: 6907 6919 7265 1972 Size: 12 346 1977 Fragments arranged by size: 2333 1977 1452 385 367 346 150 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2552 2604 2665 2856 4179 513 Size: 552 939 548 52 61 191 1323 3604 Fragments arranged by size: 3604 1323 939 552 548 191 61 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 3462 4123 487 Size: 683 56 1217 1019 661 3634 Fragments arranged by size: 3634 1217 1019 683 661 56 BtsI GCAGTG_nn' Cuts at: 3638 3970 4022 4786 5580 5600 3638 Size: 332 52 764 794 20 5308 Fragments arranged by size: 5308 794 764 332 52 20 ClaI AT'CG_AT Cuts at: 1554 3981 1554 Size: 2427 4843 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2323 2358 529 Size: 35 1759 35 5441 Fragments arranged by size: 5441 1759 35 35 DraI TTT'AAA Cuts at: 4574 4574 Size: 7270 DraIII CAC_nnn'GTG Cuts at: 2500 4017 2500 Size: 1517 5753 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 6277 809 Size: 507 942 4019 1802 Fragments arranged by size: 4019 1802 942 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 2523 3475 Size: 54 1425 50 275 18 522 171 952 Cuts at: 3475 4717 5606 7235 8 Size: 1242 889 1629 43 Fragments arranged by size: 1629 1425 1242 952 889 522 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4717 1537 Size: 275 2905 4090 Fragments arranged by size: 4090 2905 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3521 5200 1353 Size: 2168 1679 3423 Fragments arranged by size: 3423 2168 1679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2046 4460 4468 5857 6231 6377 Size: 1064 138 457 2414 8 1389 374 146 Cuts at: 6377 387 Size: 1280 Fragments arranged by size: 2414 1389 1280 1064 457 374 146 138 8 EcoICRI GAG'CTC Cuts at: 3454 3454 Size: 7270 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2425 2588 3188 3334 3577 4169 Size: 393 764 29 163 600 146 243 592 Cuts at: 4169 4175 5333 5930 6717 6985 1239 Size: 6 1158 597 787 268 1524 Fragments arranged by size: 1524 1158 787 764 600 597 592 393 268 243 163 146 29 6 EcoNI CCTnn'n_nnAGG Cuts at: 2501 4295 2501 Size: 1794 5476 EcoO109I rG'GnC_Cy Cuts at: 2038 2408 2628 2743 3243 4424 2038 Size: 370 220 115 500 1181 4884 Fragments arranged by size: 4884 1181 500 370 220 115 EcoRI G'AATT_C Cuts at: 4579 4957 4579 Size: 378 6892 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3163 3532 3163 Size: 369 6901 FspI TGC'GCA Cuts at: 5776 5776 Size: 7270 HaeII r_GCGC'y Cuts at: 1678 2167 4062 6417 1678 Size: 489 1895 2355 2531 Fragments arranged by size: 2531 2355 1895 489 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2747 2779 2786 2818 3659 Size: 32 135 32 1175 32 7 32 841 Cuts at: 3659 3691 5911 5943 5985 6017 1373 Size: 32 2220 32 42 32 2626 Fragments arranged by size: 2626 2220 1175 841 135 42 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2128 4614 4860 669 Size: 988 281 190 2486 246 3079 Fragments arranged by size: 3079 2486 988 281 246 190 HindIII A'AGCT_T Cuts at: 748 748 Size: 7270 HpaI GTT'AAC Cuts at: 4860 4860 Size: 7270 KpnI G_GTAC'C Cuts at: 4595 4595 Size: 7270 MfeI C'AATT_G Cuts at: 4869 4869 Size: 7270 MluI A'CGCG_T Cuts at: 7146 7146 Size: 7270 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2352 3690 6384 6568 1305 Size: 1047 1338 2694 184 2007 Fragments arranged by size: 2694 2007 1338 1047 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2354 10 Size: 54 1425 343 522 4926 Fragments arranged by size: 4926 1425 522 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2588 3116 3447 5228 5587 5746 518 Size: 648 1422 528 331 1781 359 159 2042 Fragments arranged by size: 2042 1781 1422 648 528 359 331 159 MspA1I CmG'CkG Cuts at: 1606 2391 2554 2643 2667 2733 3004 3021 3723 Size: 785 163 89 24 66 271 17 702 Cuts at: 3723 4389 4686 5363 6511 6756 1606 Size: 666 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 785 702 677 666 297 271 245 163 89 66 24 17 NaeI GCC'GGC Cuts at: 3475 4228 3475 Size: 753 6517 NcoI C'CATG_G Cuts at: 513 1065 2004 2856 4179 513 Size: 552 939 852 1323 3604 Fragments arranged by size: 3604 1323 939 852 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7270 NgoMIV G'CCGG_C Cuts at: 3473 4226 3473 Size: 753 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7270 NotI GC'GGCC_GC Cuts at: 4717 4717 Size: 7270 NspI r_CATG'y Cuts at: 1132 1229 3137 3905 4628 6173 1132 Size: 97 1908 768 723 1545 2229 Fragments arranged by size: 2229 1908 1545 768 723 97 PasI CC'CwG_GG Cuts at: 1348 3046 4064 1348 Size: 1698 1018 4554 Fragments arranged by size: 4554 1698 1018 PciI A'CATG_T Cuts at: 6169 6169 Size: 7270 PflMI CCAn_nnn'nTGG Cuts at: 1108 2872 4017 1108 Size: 1764 1145 4361 Fragments arranged by size: 4361 1764 1145 PmeI GTTT'AAAC Cuts at: 4574 4574 Size: 7270 PmlI CAC'GTG Cuts at: 3378 3489 3378 Size: 111 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 5339 5371 6852 6884 2315 Size: 32 2992 32 1481 32 2701 Fragments arranged by size: 2992 2701 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2628 3243 4424 2628 Size: 615 1181 5474 Fragments arranged by size: 5474 1181 615 PshAI GACnn'nnGTC Cuts at: 1613 4429 1613 Size: 2816 4454 PsiI TTA'TAA Cuts at: 4840 4840 Size: 7270 PspOMI G'GGCC_C Cuts at: 2019 2038 2407 2742 2019 Size: 19 369 335 6547 Fragments arranged by size: 6547 369 335 19 PstI C_TGCA'G Cuts at: 830 2648 3464 4622 830 Size: 1818 816 1158 3478 Fragments arranged by size: 3478 1818 1158 816 PvuI CG_AT'CG Cuts at: 2001 2017 2001 Size: 16 7254 PvuII CAG'CTG Cuts at: 2643 3004 3021 2643 Size: 361 17 6892 Fragments arranged by size: 6892 361 17 SacI G_AGCT'C Cuts at: 3456 3456 Size: 7270 SacII CC_GC'GG Cuts at: 2555 2668 2555 Size: 113 7157 SalI G'TCGA_C Cuts at: 1936 4612 1936 Size: 2676 4594 SanDI GG'GwC_CC Cuts at: 4424 4424 Size: 7270 SbfI CC_TGCA'GG Cuts at: 4622 4622 Size: 7270 ScaI AGT'ACT Cuts at: 5518 7230 5518 Size: 1712 5558 SexAI A'CCwGG_T Cuts at: 2496 2826 2496 Size: 330 6940 SfcI C'TryA_G Cuts at: 826 1045 2644 3460 3781 4618 5753 6434 6625 Size: 219 1599 816 321 837 1135 681 191 Cuts at: 6625 826 Size: 1471 Fragments arranged by size: 1599 1471 1135 837 816 681 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7270 SmaI CCC'GGG Cuts at: 1872 2160 3366 4597 1872 Size: 288 1206 1231 4545 Fragments arranged by size: 4545 1231 1206 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 3742 4085 5374 6275 6537 Size: 197 926 120 1679 343 1289 901 262 Cuts at: 6537 820 Size: 1553 Fragments arranged by size: 1679 1553 1289 926 901 343 262 197 120 SnaBI TAC'GTA Cuts at: 493 3038 5061 493 Size: 2545 2023 2702 Fragments arranged by size: 2702 2545 2023 SpeI A'CTAG_T Cuts at: 152 152 Size: 7270 SphI G_CATG'C Cuts at: 1132 3905 4628 1132 Size: 2773 723 3774 Fragments arranged by size: 3774 2773 723 SspI AAT'ATT Cuts at: 5 52 5194 5 Size: 47 5142 2081 Fragments arranged by size: 5142 2081 47 StuI AGG'CCT Cuts at: 1517 3674 1517 Size: 2157 5113 StyI C'CwwG_G Cuts at: 513 1065 2004 2856 3008 3198 3684 4179 4709 Size: 552 939 852 152 190 486 495 530 Cuts at: 4709 513 Size: 3074 Fragments arranged by size: 3074 939 852 552 530 495 486 190 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 3103 4277 5297 5314 5467 5652 Size: 1255 578 945 1174 1020 17 153 185 Cuts at: 5652 6097 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1174 1020 945 578 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2385 2620 Size: 274 80 33 51 1443 90 318 235 Cuts at: 2620 2838 4170 4518 5516 7228 96 Size: 218 1332 348 998 1712 138 Fragments arranged by size: 1712 1443 1332 998 348 318 274 235 218 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2881 2913 7230 7262 2881 Size: 32 4317 32 2889 Fragments arranged by size: 4317 2889 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7270 XbaI T'CTAG_A Cuts at: 4606 4606 Size: 7270 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3205 3635 3205 Size: 430 6840 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7270 XmaI C'CCGG_G Cuts at: 1870 2158 3364 4595 1870 Size: 288 1206 1231 4545 Fragments arranged by size: 4545 1231 1206 288 XmnI GAAnn'nnTTC Cuts at: 2048 2270 3500 5399 2048 Size: 222 1230 1899 3919 Fragments arranged by size: 3919 1899 1230 222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5078 276 Size: 53 83 186 936 3544 2468 Fragments arranged by size: 3544 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI