################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 693 GTCTAC GT^[AC][GT]AC AccIII 108 TCCGGA T^CCGGA AflIII 462 ACATGT A^C[GA][CT]GT AlwI 210 GATCC GGATC(4/5) AlwNI 345 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 320 GGGCCC GGGCC^C BalI 128 TGGCCA TGG^CCA BbvCI 342 CCTCAGC CCTCAGC(-5/-2) BciVI 651 GTATCC GTATCC(6/5) BfaI 238 CTAG C^TAG BglI 474 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 108 TCCGGA [AT]^CCGG[AT] BseAI 108 TCCGGA T^CCGGA BseRI 10 GAGGAG GAGGAG(10/8) BsgI 351 CTGCAC GTGCAG(16/14) BsmFI 388 GGGAC GGGAC(10/14) BspEI 108 TCCGGA T^CCGGA BspLU11I 462 ACATGT A^CATGT BsrDI 443 GCAATG GCAATG(2/0) BsrI 509 ACTGG ACTGG(1/-1) BstXI 273 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 210 GATC ^GATC DrdI 504 GACCAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 667 CCAAGG C^C[AT][AT]GG HaeII 338 AGCGCC [GA]GCGC^[CT] HgaI 397 GACGC GACGC(5/10) HphI 467 TCACC GGTGA(8/7) MaeI 238 CTAG C^TAG MaeII 218 ACGT A^CGT MboI 210 GATC ^GATC MluNI 128 TGGCCA TGG^CCA MroI 108 TCCGGA T^CCGGA MscI 128 TGGCCA TGG^CCA MslI 88 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 210 GATC ^GATC NspI 462 ACATGT [GA]CATG^[CT] PflMI 120 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 388 GGGACCT [GA]G^G[AT]CC[CT] PvuII 274 CAGCTG CAG^CTG Sau3AI 210 GATC ^GATC SmaI 317 CCCGGG CCC^GGG StyI 667 CCAAGG C^C[AT][AT]GG TaqI 395 TCGA T^CGA TthHB8I 395 TCGA T^CGA Van91I 120 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 6 CCAGGAGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 317 CCCGGG C^CCGGG XmaI 317 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 141 GTAC GT^AC 701 GTAC AvaI 28 CCCGAG C^[CT]CG[GA]G 317 CCCGGG BanI 415 GGTGCC G^G[CT][GA]CC 435 GGTGCC BsoBI 28 CCCGAG C^[CT]CG[GA]G 317 CCCGGG DraII 320 GGGCCCC [GA]G^G[ACGT]CC[CT] 388 GGGACCT EaeI 128 TGGCCA [CT]^GGCC[GA] 429 CGGCCA EcoNI 24 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 357 CCTGGGCCAGG EcoO109I 320 GGGCCCC [GA]G^G[ACGT]CC[CT] 388 GGGACCT MaeIII 425 GTGAC ^GT[ACGT]AC 466 GTCAC RsaI 141 GTAC GT^AC 701 GTAC StuI 234 AGGCCT AGG^CCT 582 AGGCCT Tsp45I 425 GTGAC ^GT[GC]AC 466 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI