Restriction map for
pFN21AE2229
(Circular) (Six-base) MAPSORT of: pFN21AE2229.seq Check: 7003 from: 1 to: 5440
pFN21AE2229 5440 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2699 2783 1937
Size: 762 84 4594
Fragments arranged by size:
4594 762 84
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 4887 5155 1632
Size: 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 2467 4339 5316 1203
Size: 1264 1872 977 1327
Fragments arranged by size:
1872 1327 1264 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2188 1184
Size: 1004 4436
AlwNI CAG_nnn'CTG
Cuts at: 2355 3183 4755 2355
Size: 828 1572 3040
Fragments arranged by size:
3040 1572 828
ApaI G_GGCC'C
Cuts at: 2329 2329
Size: 5440
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2322 2765 1870
Size: 73 90 289 443 4545
Fragments arranged by size:
4545 443 289 90 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 2440 2761 4216 618
Size: 325 217 627 633 20 321 1455 1842
Fragments arranged by size:
1842 1455 633 627 325 321 217 20
BanII G_rGCy'C
Cuts at: 1391 2113 2266 2303 2329 2759 1391
Size: 722 153 37 26 430 4072
Fragments arranged by size:
4072 722 430 153 37 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4469
BbvCI CC'TCA_GC
Cuts at: 2348 2348
Size: 5440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3332 4542 2667
Size: 665 1210 3565
Fragments arranged by size:
3565 1210 665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2485 4051 136
Size: 107 122 71 631 605 813 1566 1525
Fragments arranged by size:
1566 1525 813 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2212 2329 2425 2445 3501 4657 1790
Size: 422 117 96 20 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 422 117 96 20
BmgBI CAC'GTC
Cuts at: 1318 2224 1318
Size: 906 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2250 2261 2564 2585 4100 1239
Size: 1011 11 303 21 1515 2579
Fragments arranged by size:
2579 1515 1011 303 21 11
Bpu10I CC'TnA_GC
Cuts at: 2348 2460 2348
Size: 112 5328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3565 4430 4728 3565
Size: 865 298 4277
Fragments arranged by size:
4277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2113 3873 4271 4545 4692 5103 2113
Size: 1760 398 274 147 411 2450
Fragments arranged by size:
2450 1760 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 4596
BseYI C'CCAG_C
Cuts at: 2106 2381 2573 2624 4643 5139 2106
Size: 275 192 51 2019 496 2407
Fragments arranged by size:
2407 2019 496 275 192 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 1163
Size: 573 605 4262
Fragments arranged by size:
4262 605 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3501 3586 4657 3501
Size: 85 1071 4284
Fragments arranged by size:
4284 1071 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2212 2266 2303 2329 2425 2445
Size: 399 323 99 54 37 26 96 20
Cuts at: 2445 2759 3501 3586 4657 1391
Size: 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 314 99 96
85 54 37 26 20
BspEI T'CCGG_A
Cuts at: 2113 5103 2113
Size: 2990 2450
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 844
Size: 1951 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2455 3935 4109 65
Size: 1183 1207 1480 174 1396
Fragments arranged by size:
1480 1396 1207 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2285 2285
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435
Size: 798 367 385 17 1452 86 12 346
Cuts at: 5435 1972
Size: 1977
Fragments arranged by size:
1977 1452 798 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 2956 3750 3770 2172
Size: 784 794 20 3842
Fragments arranged by size:
3842 794 784 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2464 2499 529
Size: 35 1900 35 3470
Fragments arranged by size:
3470 1900 35 35
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2515 4447 809
Size: 507 1199 1932 1802
Fragments arranged by size:
1932 1802 1199 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2434 2887
Size: 54 1425 50 275 18 303 301 453
Cuts at: 2887 3776 5405 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 453 303 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2261 2564 2585 3503 4100 4887
Size: 393 618 11 303 21 918 597 787
Cuts at: 4887 5155 1239
Size: 268 1524
Fragments arranged by size:
1524 918 787 618 597 393 303 268
21 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2366 2033
Size: 333 5107
EcoO109I rG'GnC_Cy
Cuts at: 2326 2394 2326
Size: 68 5372
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2697 2697
Size: 5440
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 2347 4587 1678
Size: 669 2240 2531
Fragments arranged by size:
2531 2240 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2544 4554 4738 1305
Size: 1239 2010 184 2007
Fragments arranged by size:
2010 2007 1239 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 3315
Fragments arranged by size:
3315 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2097 3398 3757 3916 518
Size: 648 931 1301 359 159 2042
Fragments arranged by size:
2042 1301 931 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2281 2624 2856 3533 4681 4926 1606
Size: 500 175 343 232 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 500 343 245 232 175
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2471 2798 4343 1132
Size: 97 1242 327 1545 2229
Fragments arranged by size:
2229 1545 1242 327 97
PasI CC'CwG_GG
Cuts at: 1348 2303 2362 1348
Size: 955 59 4426
Fragments arranged by size:
4426 955 59
PciI A'CATG_T
Cuts at: 2467 4339 2467
Size: 1872 3568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 1108
Size: 1023 4417
PfoI T'CCnGG_A
Cuts at: 2217 2217
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 2394
Size: 5440
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2325 2325
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2792 830
Size: 1962 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2281 2281
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2788 3923 4604 4795 826
Size: 219 1743 1135 681 191 1471
Fragments arranged by size:
1743 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2324 2767 1872
Size: 452 443 4545
Fragments arranged by size:
4545 452 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3544 4445 4707 820
Size: 197 926 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SrfI GCCC'GGGC
Cuts at: 2324 2324
Size: 5440
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2241 2589 1517
Size: 724 348 4368
Fragments arranged by size:
4368 724 348
StyI C'CwwG_G
Cuts at: 513 1065 2004 2672 2879 513
Size: 552 939 668 207 3074
Fragments arranged by size:
3074 939 668 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3467 3484 3637 3822 4267 325
Size: 1255 1887 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2600 2632 5400 5432 2600
Size: 32 2768 32 2608
Fragments arranged by size:
2768 2608 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2018
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2322 2765 1870
Size: 452 443 4545
Fragments arranged by size:
4545 452 443
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SwaI