(Linear) (Six-base) MAPSORT of: KIEE2229.seq Check: 1599 from: 1 to: 735 KIEE2229.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AccI GT'mk_AC Cuts at: 0 694 735 Size: 694 41 AflIII A'CryG_T Cuts at: 0 462 735 Size: 462 273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 183 735 Size: 183 552 AlwNI CAG_nnn'CTG Cuts at: 0 350 735 Size: 350 385 ApaI G_GGCC'C Cuts at: 0 324 735 Size: 324 411 AvaI C'yCGr_G Cuts at: 0 28 317 735 Size: 28 289 418 Fragments arranged by size: 418 289 28 BanI G'GyrC_C Cuts at: 0 415 435 735 Size: 415 20 300 Fragments arranged by size: 415 300 20 BanII G_rGCy'C Cuts at: 0 108 261 298 324 735 Size: 108 153 37 26 411 Fragments arranged by size: 411 153 108 37 26 BbvCI CC'TCA_GC Cuts at: 0 343 735 Size: 343 392 BciVI GTATCCnnnnn_n' Cuts at: 0 662 735 Size: 662 73 BglI GCCn_nnn'nGGC Cuts at: 0 480 735 Size: 480 255 Bme1580I G_kGCm'C Cuts at: 0 207 324 420 440 735 Size: 207 117 96 20 295 Fragments arranged by size: 295 207 117 96 20 BmgBI CAC'GTC Cuts at: 0 219 735 Size: 219 516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 256 559 580 735 Size: 245 11 303 21 155 Fragments arranged by size: 303 245 155 21 11 Bpu10I CC'TnA_GC Cuts at: 0 343 455 735 Size: 343 112 280 Fragments arranged by size: 343 280 112 BsaWI w'CCGG_w Cuts at: 0 108 735 Size: 108 627 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 735 Size: 25 710 BseYI C'CCAG_C Cuts at: 0 101 376 568 619 735 Size: 101 275 192 51 116 Fragments arranged by size: 275 192 116 101 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 735 Size: 336 399 Bsp1286I G_dGCh'C Cuts at: 0 108 207 261 298 324 420 440 735 Size: 108 99 54 37 26 96 20 295 Fragments arranged by size: 295 108 99 96 54 37 26 20 BspEI T'CCGG_A Cuts at: 0 108 735 Size: 108 627 BsrDI GCAATG_nn' Cuts at: 0 450 735 Size: 450 285 BstXI CCAn_nnnn'nTGG Cuts at: 0 280 735 Size: 280 455 BtsI GCAGTG_nn' Cuts at: 0 167 735 Size: 167 568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 459 494 735 Size: 459 35 241 Fragments arranged by size: 459 241 35 DrdI GACnn_nn'nnGTC Cuts at: 0 510 735 Size: 510 225 EaeI y'GGCC_r Cuts at: 0 128 429 735 Size: 128 301 306 Fragments arranged by size: 306 301 128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 256 559 580 735 Size: 245 11 303 21 155 Fragments arranged by size: 303 245 155 21 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 28 361 735 Size: 28 333 374 Fragments arranged by size: 374 333 28 EcoO109I rG'GnC_Cy Cuts at: 0 321 389 735 Size: 321 68 346 Fragments arranged by size: 346 321 68 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 692 735 Size: 692 43 HaeII r_GCGC'y Cuts at: 0 342 735 Size: 342 393 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 539 735 Size: 539 196 MscI TGG'CCA Cuts at: 0 130 735 Size: 130 605 MslI CAynn'nnrTG Cuts at: 0 92 735 Size: 92 643 MspA1I CmG'CkG Cuts at: 0 101 276 619 735 Size: 101 175 343 116 Fragments arranged by size: 343 175 116 101 NspI r_CATG'y Cuts at: 0 466 735 Size: 466 269 PasI CC'CwG_GG Cuts at: 0 298 357 735 Size: 298 59 378 Fragments arranged by size: 378 298 59 PciI A'CATG_T Cuts at: 0 462 735 Size: 462 273 PflMI CCAn_nnn'nTGG Cuts at: 0 126 735 Size: 126 609 PfoI T'CCnGG_A Cuts at: 0 212 735 Size: 212 523 PpuMI rG'GwC_Cy Cuts at: 0 389 735 Size: 389 346 PspOMI G'GGCC_C Cuts at: 0 320 735 Size: 320 415 PvuII CAG'CTG Cuts at: 0 276 735 Size: 276 459 SmaI CCC'GGG Cuts at: 0 319 735 Size: 319 416 SrfI GCCC'GGGC Cuts at: 0 319 735 Size: 319 416 StuI AGG'CCT Cuts at: 0 236 584 735 Size: 236 348 151 Fragments arranged by size: 348 236 151 StyI C'CwwG_G Cuts at: 0 667 735 Size: 667 68 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 595 627 735 Size: 595 32 108 Fragments arranged by size: 595 108 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 13 735 Size: 13 722 XmaI C'CCGG_G Cuts at: 0 317 735 Size: 317 418 Enzymes that do cut: AccI AflIII AlfI AlwNI ApaI AvaI BanI BanII BbvCI BciVI BglI Bme1580I BmgBI BpmI Bpu10I BsaWI BseRI BseYI BsgI Bsp1286I BspEI BsrDI BstXI BtsI CspCI DrdI EaeI Eco57MI EcoNI EcoO109I FalI HaeII MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII SmaI SrfI StuI StyI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI