(Circular) (Six-base) MAPSORT of: pFN21AB5775.seq Check: 5805 from: 1 to: 7144 pFN21AB5775 7144 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4954 278 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4487 1937 Size: 2550 4594 Acc65I G'GTAC_C Cuts at: 4465 4465 Size: 7144 AclI AA'CG_TT Cuts at: 1563 5271 5644 1563 Size: 3708 373 3063 Fragments arranged by size: 3708 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 2303 2345 2373 3945 5207 6591 6859 Size: 645 26 42 28 1572 1262 1384 268 Cuts at: 6859 1632 Size: 1917 Fragments arranged by size: 1917 1572 1384 1262 645 268 42 28 26 AflII C'TTAA_G Cuts at: 820 1017 2240 3746 820 Size: 197 1223 1506 4218 Fragments arranged by size: 4218 1506 1223 197 AflIII A'CryG_T Cuts at: 1203 3446 6043 7020 1203 Size: 2243 2597 977 1327 Fragments arranged by size: 2597 2243 1327 977 AgeI A'CCGG_T Cuts at: 5975 5975 Size: 7144 AhdI GACnn_n'nnGTC Cuts at: 5873 5873 Size: 7144 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7144 AlwNI CAG_nnn'CTG Cuts at: 2035 4887 6459 2035 Size: 2852 1572 2720 Fragments arranged by size: 2852 2720 1572 ApaLI G'TGCA_C Cuts at: 5201 6357 5201 Size: 1156 5988 ApoI r'AATT_y Cuts at: 1493 2600 3226 3509 4431 4453 4679 4831 6940 Size: 1107 626 283 922 22 226 152 2109 Cuts at: 6940 1493 Size: 1697 Fragments arranged by size: 2109 1697 1107 922 626 283 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 5698 160 Size: 872 4666 1606 Fragments arranged by size: 4666 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 AvaI C'yCGr_G Cuts at: 1870 1943 2150 4469 1870 Size: 73 207 2319 4545 Fragments arranged by size: 4545 2319 207 73 BamHI G'GATC_C Cuts at: 4474 4841 4474 Size: 367 6777 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2199 2833 4465 5920 Size: 325 217 627 359 53 634 1632 1455 Cuts at: 5920 618 Size: 1842 Fragments arranged by size: 1842 1632 1455 634 627 359 325 217 53 BanII G_rGCy'C Cuts at: 1391 2136 4463 1391 Size: 745 2327 4072 Fragments arranged by size: 4072 2327 745 BbeI G_GCGC'C Cuts at: 2150 2203 2150 Size: 53 7091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4012 4148 928 Size: 971 2113 136 3924 Fragments arranged by size: 3924 2113 971 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5335 5369 1903 Size: 34 3398 34 3678 Fragments arranged by size: 3678 3398 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5036 6246 5036 Size: 1210 5934 BclI T'GATC_A Cuts at: 1548 3807 1548 Size: 2259 4885 BfrBI ATG'CAT Cuts at: 4123 4325 4123 Size: 202 6942 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 5755 136 Size: 107 122 71 631 605 406 3677 1525 Fragments arranged by size: 3677 1525 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 7139 7139 Size: 7144 BlpI GC'TnA_GC Cuts at: 2126 4561 2126 Size: 2435 4709 Bme1580I G_kGCm'C Cuts at: 1790 5205 6361 1790 Size: 3415 1156 2573 Fragments arranged by size: 3415 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4875 5833 442 Size: 416 53 83 399 24 3458 958 1753 Fragments arranged by size: 3458 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7144 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2185 2246 2270 4302 5804 1239 Size: 946 61 24 2032 1502 2579 Fragments arranged by size: 2579 2032 1502 946 61 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5269 6134 6432 5269 Size: 865 298 5981 Fragments arranged by size: 5981 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 5807 882 Size: 617 1308 3000 2219 Fragments arranged by size: 3000 2219 1308 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 4935 493 Size: 711 2243 1488 2702 Fragments arranged by size: 2702 2243 1488 711 BsaBI GATnn'nnATC Cuts at: 3894 7138 3894 Size: 3244 3900 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2200 2667 4951 Size: 53 83 186 936 614 53 467 2284 Cuts at: 4951 5333 5968 275 Size: 382 635 1451 Fragments arranged by size: 2284 1451 936 635 614 467 382 186 83 53 53 BsaWI w'CCGG_w Cuts at: 3838 5577 5975 6249 6396 6807 3838 Size: 1739 398 274 147 411 4175 Fragments arranged by size: 4175 1739 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6919 Fragments arranged by size: 6919 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 4221 1186 Size: 996 2039 4109 Fragments arranged by size: 4109 2039 996 BseYI C'CCAG_C Cuts at: 2267 6347 6843 2267 Size: 4080 496 2568 Fragments arranged by size: 4080 2568 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6571 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2082 4594 5355 6383 1540 Size: 275 186 48 33 2512 761 1028 2301 Fragments arranged by size: 2512 2301 1028 761 275 186 48 33 BsiHKAI G_wGCw'C Cuts at: 2136 3039 3649 5205 5290 6361 2136 Size: 903 610 1556 85 1071 2919 Fragments arranged by size: 2919 1556 1071 903 610 85 BsmI GAATG_Cn' Cuts at: 3478 4655 4748 3478 Size: 1177 93 5874 Fragments arranged by size: 5874 1177 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2674 2674 Size: 7144 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 3039 3649 4463 5205 5290 6361 Size: 399 346 903 610 814 742 85 1071 Cuts at: 6361 1391 Size: 2174 Fragments arranged by size: 2174 1071 903 814 742 610 399 346 85 BspEI T'CCGG_A Cuts at: 6807 6807 Size: 7144 BspHI T'CATG_A Cuts at: 3721 3804 5033 6763 3721 Size: 83 1229 1730 4102 Fragments arranged by size: 4102 1730 1229 83 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4499 844 Size: 3655 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5031 1394 Size: 429 3208 3507 Fragments arranged by size: 3507 3208 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3967 4104 4226 4303 5639 5813 65 Size: 1183 2719 137 122 77 1336 174 1396 Fragments arranged by size: 2719 1396 1336 1183 174 137 122 77 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 5788 5975 1700 Size: 110 271 3707 187 2869 Fragments arranged by size: 3707 2869 271 187 110 BsrGI T'GTAC_A Cuts at: 96 3295 96 Size: 3199 3945 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7144 BssSI C'ACGA_G Cuts at: 5204 6216 5204 Size: 1012 6132 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7144 BstBI TT'CG_AA Cuts at: 3622 5948 3622 Size: 2326 4818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7144 BstXI CCAn_nnnn'nTGG Cuts at: 3482 3482 Size: 7144 BstYI r'GATC_y Cuts at: 1972 3124 3955 4474 4841 5226 5243 6695 6781 Size: 1152 831 519 367 385 17 1452 86 Cuts at: 6781 6793 7139 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1152 831 519 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4201 4201 Size: 7144 BtgI C'CryG_G Cuts at: 513 1065 2055 4243 513 Size: 552 990 2188 3414 Fragments arranged by size: 3414 2188 990 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6405 Fragments arranged by size: 6405 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2547 3992 4660 5454 5474 2237 Size: 310 1445 668 794 20 3907 Fragments arranged by size: 3907 1445 794 668 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7144 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2462 2497 529 Size: 35 1898 35 5176 Fragments arranged by size: 5176 1898 35 35 DraI TTT'AAA Cuts at: 3516 4448 3516 Size: 932 6212 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6151 809 Size: 507 4835 1802 Fragments arranged by size: 4835 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2079 4591 Size: 54 1425 50 275 18 216 33 2512 Cuts at: 4591 5480 7109 8 Size: 889 1629 43 Fragments arranged by size: 2512 1629 1425 889 275 216 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2079 4591 1537 Size: 275 234 33 2512 4090 Fragments arranged by size: 4090 2512 275 234 33 EarI CTCTTCn'nnn_ Cuts at: 1353 3635 5074 1353 Size: 2282 1439 3423 Fragments arranged by size: 3423 2282 1439 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 2195 5731 6105 6251 387 Size: 1064 138 603 3 3536 374 146 1280 Fragments arranged by size: 3536 1280 1064 603 374 146 138 3 EcoICRI GAG'CTC Cuts at: 2134 2134 Size: 7144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2246 2270 2277 2303 2345 2373 Size: 393 553 61 24 7 26 42 28 Cuts at: 2373 3945 4302 5207 5804 6591 6859 1239 Size: 1572 357 905 597 787 268 1524 Fragments arranged by size: 1572 1524 905 787 597 553 393 357 268 61 42 28 26 24 7 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2141 Size: 7144 EcoO109I rG'GnC_Cy Cuts at: 2020 2032 2020 Size: 12 7132 EcoRI G'AATT_C Cuts at: 2600 4453 4831 2600 Size: 1853 378 4913 Fragments arranged by size: 4913 1853 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 4161 4165 2434 Size: 1727 4 5413 Fragments arranged by size: 5413 1727 4 FspI TGC'GCA Cuts at: 5650 5650 Size: 7144 HaeII r_GCGC'y Cuts at: 1678 2150 2203 6291 1678 Size: 472 53 4088 2531 Fragments arranged by size: 4088 2531 472 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2654 2686 5785 5817 5859 Size: 32 135 32 1082 32 3099 32 42 Cuts at: 5859 5891 1373 Size: 32 2626 Fragments arranged by size: 3099 2626 1082 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4488 4734 669 Size: 988 281 2550 246 3079 Fragments arranged by size: 3079 2550 988 281 246 HindIII A'AGCT_T Cuts at: 748 3462 748 Size: 2714 4430 HpaI GTT'AAC Cuts at: 4734 4734 Size: 7144 KasI G'GCGC_C Cuts at: 2146 2199 2146 Size: 53 7091 KpnI G_GTAC'C Cuts at: 4469 4469 Size: 7144 MfeI C'AATT_G Cuts at: 4743 4743 Size: 7144 MluI A'CGCG_T Cuts at: 7020 7020 Size: 7144 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 3102 6258 6442 1305 Size: 1432 365 3156 184 2007 Fragments arranged by size: 3156 2007 1432 365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5322 Fragments arranged by size: 5322 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3219 5102 5461 5620 518 Size: 648 2053 1883 359 159 2042 Fragments arranged by size: 2053 2042 1883 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2113 2620 3097 3788 4560 5237 6385 Size: 459 48 507 477 691 772 677 1148 Cuts at: 6385 6630 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 772 691 677 507 477 459 245 48 NaeI GCC'GGC Cuts at: 2083 2083 Size: 7144 NarI GG'CG_CC Cuts at: 2147 2200 2147 Size: 53 7091 NcoI C'CATG_G Cuts at: 513 1065 4243 513 Size: 552 3178 3414 Fragments arranged by size: 3414 3178 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7144 NgoMIV G'CCGG_C Cuts at: 2081 2081 Size: 7144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7144 NotI GC'GGCC_GC Cuts at: 4591 4591 Size: 7144 NsiI A_TGCA'T Cuts at: 4125 4327 4125 Size: 202 6942 NspI r_CATG'y Cuts at: 1132 1229 2549 4123 4502 6047 1132 Size: 97 1320 1574 379 1545 2229 Fragments arranged by size: 2229 1574 1545 1320 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7144 PciI A'CATG_T Cuts at: 6043 6043 Size: 7144 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 4244 1108 Size: 1878 1258 4008 Fragments arranged by size: 4008 1878 1258 PfoI T'CCnGG_A Cuts at: 2136 2136 Size: 7144 PmeI GTTT'AAAC Cuts at: 4448 4448 Size: 7144 PmlI CAC'GTG Cuts at: 3447 3447 Size: 7144 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5213 5245 6726 6758 5213 Size: 32 1481 32 5599 Fragments arranged by size: 5599 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2020 Size: 7144 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7144 PsiI TTA'TAA Cuts at: 4714 4714 Size: 7144 PstI C_TGCA'G Cuts at: 830 2337 4195 4496 830 Size: 1507 1858 301 3478 Fragments arranged by size: 3478 1858 1507 301 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7144 PvuII CAG'CTG Cuts at: 2620 3097 3788 2620 Size: 477 691 5976 Fragments arranged by size: 5976 691 477 SacI G_AGCT'C Cuts at: 2136 2136 Size: 7144 SalI G'TCGA_C Cuts at: 1936 4486 1936 Size: 2550 4594 SanDI GG'GwC_CC Cuts at: 2020 2020 Size: 7144 SbfI CC_TGCA'GG Cuts at: 2337 4496 2337 Size: 2159 4985 ScaI AGT'ACT Cuts at: 4046 5392 7104 4046 Size: 1346 1712 4086 Fragments arranged by size: 4086 1712 1346 SfcI C'TryA_G Cuts at: 826 1045 2027 2333 3704 4065 4191 4492 5627 Size: 219 982 306 1371 361 126 301 1135 Cuts at: 5627 6308 6499 826 Size: 681 191 1471 Fragments arranged by size: 1471 1371 1135 982 681 361 306 301 219 191 126 SfoI GGC'GCC Cuts at: 2148 2201 2148 Size: 53 7091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7144 SmaI CCC'GGG Cuts at: 1872 4471 1872 Size: 2599 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 3746 5248 6149 6411 820 Size: 197 926 297 1506 1502 901 262 1553 Fragments arranged by size: 1553 1506 1502 926 901 297 262 197 SnaBI TAC'GTA Cuts at: 493 4935 493 Size: 4442 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7144 SphI G_CATG'C Cuts at: 1132 4502 1132 Size: 3370 3774 SspI AAT'ATT Cuts at: 5 52 3427 3677 5068 5 Size: 47 3375 250 1391 2081 Fragments arranged by size: 3375 2081 1391 250 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7144 StyI C'CwwG_G Cuts at: 513 1065 2857 3714 4243 4583 513 Size: 552 1792 857 529 340 3074 Fragments arranged by size: 3074 1792 857 552 529 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 5171 5188 5341 5526 5971 325 Size: 1255 702 2889 17 153 185 445 1498 Fragments arranged by size: 2889 1498 1255 702 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3295 4044 5390 Size: 274 80 33 51 1443 1318 749 1346 Cuts at: 5390 7102 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1346 1318 749 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2418 2450 3327 3359 7104 7136 2418 Size: 32 877 32 3745 32 2426 Fragments arranged by size: 3745 2426 877 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7144 XbaI T'CTAG_A Cuts at: 3613 3886 4480 3613 Size: 273 594 6277 Fragments arranged by size: 6277 594 273 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3397 4262 2011 Size: 1386 865 4893 Fragments arranged by size: 4893 1386 865 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7144 XmaI C'CCGG_G Cuts at: 1870 4469 1870 Size: 2599 4545 XmnI GAAnn'nnTTC Cuts at: 2358 3239 5273 2358 Size: 881 2034 4229 Fragments arranged by size: 4229 2034 881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4952 276 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI