Restriction map for
pFN21AE1481
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 864 CCGC CCGC(-3/-1)
AcsI 913 GAATTC [GA]^AATT[CT]
AfaI 744 GTAC GT^AC
AlwI 657 GGATC GGATC(4/5)
AlwNI 958 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 913 GAATTC [GA]^AATT[CT]
AvaI 523 CCCGAG C^[CT]CG[GA]G
BanII 991 GAGCCC G[GA]GC[CT]^C
BbsI 22 GAAGAC GAAGAC(2/6)
BbvCI 937 GCTGAGG CCTCAGC(-5/-2)
BclI 533 TGATCA T^GATCA
BpuAI 22 GAAGAC GAAGAC(2/6)
BsaBI 361 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 800 GAGACC GGTCTC(1/5)
BsmFI 236 GGGAC GGGAC(10/14)
BsoBI 523 CCCGAG C^[CT]CG[GA]G
BstYI 657 GGATCT [GA]^GATC[CT]
DrdI 309 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 913 GAATTC G^AATTC
FbaI 533 TGATCA T^GATCA
HindIII 793 AAGCTT A^AGCTT
MamI 361 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 657 GGATCT [GA]^GATC[CT]
MseI 227 TTAA T^TAA
MspA1I 568 CAGCTG C[AC]G^C[GT]G
NspI 629 ACATGC [GA]CATG^[CT]
PleI 25 GACTC GAGTC(4/5)
PpuMI 236 GGGACCT [GA]G^G[AT]CC[CT]
PstI 406 CTGCAG CTGCA^G
PvuII 568 CAGCTG CAG^CTG
RsaI 744 GTAC GT^AC
SmlI 352 CTCAAG C^T[CT][GA]AG
Tru9I 227 TTAA T^TAA
Tsp509I 914 AATT ^AATT
XbaI 18 TCTAGA T^CTAGA
XhoII 657 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 118 CCTGAGG CC^T[ACGT]AGG
753 CCTGAGG
AvaII 237 GGACC G^G[AT]CC
519 GGACC
BalI 770 TGGCCA TGG^CCA
788 TGGCCA
BfaI 19 CTAG C^TAG
416 CTAG
BmyI 380 GTGCCC G[AGT]GC[ACT]^C
991 GAGCCC
BpmI 720 CTCCAG CTGGAG(16/14)
220 CTGGAG
BsgI 541 CTGCAC GTGCAG(16/14)
880 GTGCAG
Bsp1286I 380 GTGCCC G[AGT]GC[ACT]^C
991 GAGCCC
BsrDI 737 CATTGC GCAATG(2/0)
191 GCAATG
Bsu36I 118 CCTGAGG CC^T[ACGT]AGG
753 CCTGAGG
CfoI 261 GCGC GCG^C
279 GCGC
DraII 236 GGGACCT [GA]G^G[ACGT]CC[CT]
872 AGGCCCT
DraIII 502 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
544 CACACAGTG
EaeI 770 TGGCCA [CT]^GGCC[GA]
788 TGGCCA
EarI 58 GAAGAG CTCTTC(1/4)
109 GAAGAG
Eco81I 118 CCTGAGG CC^T[ACGT]AGG
753 CCTGAGG
EcoNI 141 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
903 CCTGGACAAGG
EcoO109I 236 GGGACCT [GA]G^G[ACGT]CC[CT]
872 AGGCCCT
HhaI 261 GCGC GCG^C
279 GCGC
HinP1I 261 GCGC G^CGC
279 GCGC
Ksp632I 58 GAAGAG CTCTTC(1/4)
109 GAAGAG
MaeI 19 CTAG C^TAG
416 CTAG
MaeIII 508 GTGAC ^GT[ACGT]AC
1001 GTGAC
MluNI 770 TGGCCA TGG^CCA
788 TGGCCA
MscI 770 TGGCCA TGG^CCA
788 TGGCCA
MslI 205 CATGCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CATCCACATG
SfcI 93 CTACAG C^T[GA][CT]AG
406 CTGCAG
Tsp45I 508 GTGAC ^GT[GC]AC
1001 GTGAC
TspRI 444 CACTG CA[GC]TG[ACGT][ACGT]^
548 CAGTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbvI BsaJI BseRI BsiYI
BslI BsmAI BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI TthHB8I