(Circular) (Six-base) MAPSORT of: pFN21AE1481.seq Check: 151 from: 1 to: 5734 pFN21AE1481 5734 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2355 2355 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 2630 2660 2990 3797 5181 5449 1632 Size: 840 158 30 330 807 1384 268 1917 Fragments arranged by size: 1917 1384 840 807 330 268 158 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2393 1184 Size: 1209 4525 AlwNI CAG_nnn'CTG Cuts at: 2968 3477 5049 2968 Size: 509 1572 3653 Fragments arranged by size: 3653 1572 509 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 2918 3043 3269 3421 5530 1493 Size: 1425 125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1425 226 152 125 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2528 3059 1870 Size: 73 585 531 4545 Fragments arranged by size: 4545 585 531 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 4510 618 Size: 325 217 627 1268 1455 1842 Fragments arranged by size: 1842 1455 1268 627 325 217 BanII G_rGCy'C Cuts at: 1391 3000 3053 1391 Size: 1609 53 4072 Fragments arranged by size: 4072 1609 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 928 Size: 971 135 4628 Fragments arranged by size: 4628 971 135 BbvCI CC'TCA_GC Cuts at: 2943 2943 Size: 5734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 2538 1548 Size: 990 4744 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2389 3795 4951 1790 Size: 599 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 599 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2710 4394 1239 Size: 1007 464 1684 2579 Fragments arranged by size: 2579 1684 1007 464 Bpu10I CC'TnA_GC Cuts at: 2604 2943 2994 2604 Size: 339 51 5344 Fragments arranged by size: 5344 339 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 3859 4724 5022 2342 Size: 1517 865 298 3054 Fragments arranged by size: 3054 1517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2799 4397 882 Size: 617 1300 1598 2219 Fragments arranged by size: 2219 1598 1300 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 2370 5728 2370 Size: 3358 2376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5509 Fragments arranged by size: 5509 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2942 2945 1186 Size: 913 843 3 3975 Fragments arranged by size: 3975 913 843 3 BseYI C'CCAG_C Cuts at: 3000 4937 5433 3000 Size: 1937 496 3301 Fragments arranged by size: 3301 1937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2531 2906 1163 Size: 573 795 375 3991 Fragments arranged by size: 3991 795 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2389 3000 3053 3795 3880 4951 1391 Size: 399 599 611 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 599 399 85 53 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2355 2418 2817 3089 844 Size: 1511 63 399 272 3489 Fragments arranged by size: 3489 1511 399 272 63 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2203 2741 4229 4403 65 Size: 1183 955 538 1488 174 1396 Fragments arranged by size: 1488 1396 1183 955 538 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2662 3064 3431 3816 3833 5285 5371 5383 Size: 690 402 367 385 17 1452 86 12 Cuts at: 5383 5729 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 690 402 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2124 2759 2124 Size: 635 5099 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 3250 4044 4064 3250 Size: 794 20 4920 Fragments arranged by size: 4920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DraIII CAC_nnn'GTG Cuts at: 2512 2554 2512 Size: 42 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2320 4741 809 Size: 507 1004 2421 1802 Fragments arranged by size: 2421 1802 1004 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2775 2793 3181 Size: 54 1425 50 275 18 945 18 388 Cuts at: 3181 4070 5699 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 945 889 388 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2109 3664 1353 Size: 705 51 1555 3423 Fragments arranged by size: 3423 1555 705 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2472 2630 2660 2710 2990 3797 Size: 393 614 226 158 30 50 280 807 Cuts at: 3797 4394 5181 5449 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 614 597 393 280 268 226 158 50 30 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2912 2150 Size: 762 4972 EcoO109I rG'GnC_Cy Cuts at: 2242 2878 2242 Size: 636 5098 EcoRI G'AATT_C Cuts at: 2918 3043 3421 2918 Size: 125 378 5231 Fragments arranged by size: 5231 378 125 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373 Size: 32 135 32 2803 32 42 32 2626 Fragments arranged by size: 2803 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 2798 748 Size: 2050 3684 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2777 2795 10 Size: 54 1425 343 945 18 2949 Fragments arranged by size: 2949 1425 945 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2214 2507 3692 4051 4210 518 Size: 648 1048 293 1185 359 159 2042 Fragments arranged by size: 2042 1185 1048 648 359 293 159 MspA1I CmG'CkG Cuts at: 1606 2575 3150 3827 4975 5220 1606 Size: 969 575 677 1148 245 2120 Fragments arranged by size: 2120 1148 969 677 575 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2638 3092 4637 1132 Size: 97 1409 454 1545 2229 Fragments arranged by size: 2229 1545 1409 454 97 PasI CC'CwG_GG Cuts at: 1348 2587 1348 Size: 1239 4495 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2242 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 2433 3304 2433 Size: 871 4863 PstI C_TGCA'G Cuts at: 830 2415 3086 830 Size: 1585 671 3478 Fragments arranged by size: 3478 1585 671 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2575 2575 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2098 2411 3082 4217 4898 5089 826 Size: 219 1053 313 671 1135 681 191 1471 Fragments arranged by size: 1471 1135 1053 681 671 313 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2357 3838 4739 5001 820 Size: 197 926 414 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 414 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 3173 513 Size: 552 2108 3074 Fragments arranged by size: 3074 2108 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 3761 3778 3931 4116 4561 325 Size: 1255 777 1404 17 153 185 445 1498 Fragments arranged by size: 1498 1404 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 2023 3070 2023 Size: 1047 4687 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI