Restriction map for
pFN21AB7961
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HapII Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MflI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AlwI 82 GATCC GGATC(4/5)
BanII 692 GAGCCC G[GA]GC[CT]^C
BbsI 784 GAAGAC GAAGAC(2/6)
BglI 335 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 692 GAGCCC G[AGT]GC[ACT]^C
BpuAI 784 GAAGAC GAAGAC(2/6)
BsaI 539 GAGACC GGTCTC(1/5)
BsaJI 629 CCCTGG C^C[ACGT][ACGT]GG
BseRI 313 GAGGAG GAGGAG(10/8)
BsgI 297 GTGCAG GTGCAG(16/14)
BsiYI 201 CCATGTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 201 CCATGTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 539 GAGAC GTCTC(1/5)
BsmFI 645 GGGAC GGGAC(10/14)
BsmI 572 GCATTC GAATGC(1/-1)
Bsp1286I 692 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 749 TGTACA T^GTACA
BspMI 542 ACCTGC ACCTGC(4/8)
BsrBI 727 GAGCGG CCGCTC(-3/-3)
BsrDI 113 GCAATG GCAATG(2/0)
BsrGI 749 TGTACA T^GTACA
BssKI 630 CCTGG ^CC[ACGT]GG
BstNI 630 CCTGG CC^[AT]GG
CfoI 198 GCGC GCG^C
EarI 118 GAAGAG CTCTTC(1/4)
EcoRII 630 CCTGG ^CC[AT]GG
EcoT22I 68 ATGCAT ATGCA^T
FauI 729 GCGGG CCCGC(4/6)
HaeII 197 AGCGCC [GA]GCGC^[CT]
HgaI 403 GACGC GACGC(5/10)
HhaI 198 GCGC GCG^C
HinP1I 198 GCGC G^CGC
HincII 733 GTTGAC GT[CT]^[GA]AC
HindII 733 GTTGAC GT[CT]^[GA]AC
HphI 42 GGTGA GGTGA(8/7)
Ksp632I 118 GAAGAG CTCTTC(1/4)
MfeI 252 CAATTG C^AATTG
MslI 770 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 252 CAATTG C^AATTG
MvaI 630 CCTGG CC^[AT]GG
NdeI 674 CATATG CA^TATG
NlaIV 215 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 68 ATGCAT ATGCA^T
PshAI 168 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 289 CTGCAG CTGCA^G
ScaI 122 AGTACT AGT^ACT
ScrFI 630 CCTGG CC^[ACGT]GG
SfcI 289 CTGCAG C^T[GA][CT]AG
SspBI 749 TGTACA T^GTACA
TaqI 323 TCGA T^CGA
TfiI 442 GATTC G^A[AT]TC
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 323 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BpmI 217 CTCCAG CTGGAG(16/14)
364 CTCCAG
Cac8I 287 GCCTGC GC[ACGT]^[ACGT]GC
331 GCTGGC
DdeI 194 CTAAG C^T[ACGT]AG
697 CTGAG
DpnII 82 GATC ^GATC
103 GATC
MboI 82 GATC ^GATC
103 GATC
MseI 398 TTAA T^TAA
617 TTAA
MspA1I 292 CAGCTG C[AC]G^C[GT]G
329 CAGCTG
MwoI 3 GCCATCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
335 GCCTCTGCGGC
NdeII 82 GATC ^GATC
103 GATC
NlaIII 202 CATG CATG^
303 CATG
PleI 37 GACTC GAGTC(4/5)
157 GACTC
PvuII 292 CAGCTG CAG^CTG
329 CAGCTG
Sau3AI 82 GATC ^GATC
103 GATC
Tru9I 398 TTAA T^TAA
617 TTAA
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsrI BstF5I Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboII
MnlI RsaI SfaNI TseI Tsp45I
Tsp509I