Restriction map for
pFN21AE2543
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstBI
BstEII BstNI BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoT14I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI Ksp632I MamI MfeI MluI
MroI MslI MspI MunI MvaI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI ScrFI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 16 CGCG CG^CG
AfaI 43 GTAC GT^AC
AflII 111 CTTAAG C^TTAAG
AlwI 84 GGATC GGATC(4/5)
Aor51HI 315 AGCGCT AGC^GCT
AseI 504 ATTAAT AT^TAAT
AsnI 504 ATTAAT AT^TAAT
AvaI 92 CCCGAG C^[CT]CG[GA]G
AvaII 549 GGTCC G^G[AT]CC
BbvI 301 GCAGC GCAGC(8/12)
BfrI 111 CTTAAG C^TTAAG
BsaJI 15 CCGCGG C^C[ACGT][ACGT]GG
BseRI 67 GAGGAG GAGGAG(10/8)
BsiYI 126 CCAAAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 126 CCAAAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 539 GAGAC GTCTC(1/5)
BsmFI 76 GTCCC GGGAC(10/14)
BsmI 468 GAATGC GAATGC(1/-1)
BsoBI 92 CCCGAG C^[CT]CG[GA]G
BspDI 646 ATCGAT AT^CGAT
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BsrDI 561 CATTGC GCAATG(2/0)
BstAPI 556 GCATGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 16 CGCG CG^CG
BstYI 84 GGATCT [GA]^GATC[CT]
CfoI 316 GCGC GCG^C
ClaI 646 ATCGAT AT^CGAT
DdeI 191 CTAAG C^T[ACGT]AG
DraI 781 TTTAAA TTT^AAA
DraII 674 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 541 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 15 CCGCGG C^C[GA][CT]GG
Eco47III 315 AGCGCT AGC^GCT
EcoO109I 674 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 558 ATGCAT ATGCA^T
FauI 78 CCCGC CCCGC(4/6)
HaeII 315 AGCGCT [GA]GCGC^[CT]
HhaI 316 GCGC GCG^C
HinP1I 316 GCGC G^CGC
HindIII 193 AAGCTT A^AGCTT
KspI 15 CCGCGG CCGC^GG
MaeII 74 ACGT A^CGT
MaeIII 493 GTGAC ^GT[ACGT]AC
MflI 84 GGATCT [GA]^GATC[CT]
MvnI 16 CGCG CG^CG
NlaIII 557 CATG CATG^
NotI 17 GCGGCCGC GC^GGCCGC
NsiI 558 ATGCAT ATGCA^T
NspI 556 GCATGC [GA]CATG^[CT]
PflMI 126 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 504 ATTAAT AT^TAAT
PvuI 8 CGATCG CGAT^CG
RsaI 43 GTAC GT^AC
SacII 15 CCGCGG CCGC^GG
SmlI 111 CTTAAG C^T[CT][GA]AG
SphI 556 GCATGC GCATG^C
TseI 301 GCAGC G^C[AT]GC
Tsp45I 493 GTGAC ^GT[GC]AC
TspRI 216 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 126 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 84 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BalI 134 TGGCCA TGG^CCA
210 TGGCCA
BpmI 339 CTCCAG CTGGAG(16/14)
349 CTCCAG
BsrI 228 ACTGG ACTGG(1/-1)
341 CCAGT
BstF5I 330 CATCC GGATG(2/0)
709 CATCC
EagI 12 CGGCCG C^GGCCG
18 CGGCCG
EclXI 12 CGGCCG C^GGCCG
18 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
18 CGGCCG
EcoRV 247 GATATC GAT^ATC
323 GATATC
FokI 330 CATCC GGATG(9/13)
709 CATCC
MboII 30 GAAGA GAAGA(8/7)
220 GAAGA
MluNI 134 TGGCCA TGG^CCA
210 TGGCCA
MscI 134 TGGCCA TGG^CCA
210 TGGCCA
PleI 337 GACTC GAGTC(4/5)
717 GACTC
SspI 765 AATATT AAT^ATT
786 AATATT
TfiI 120 GATTC G^A[AT]TC
591 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BfaI BsiEI Cac8I
Cfr13I DpnII EaeI Fnu4HI HaeIII
HinfI ItaI MaeI MboI MnlI
MseI MspA1I MwoI NdeII Sau3AI
Sau96I TaqI Tru9I Tsp509I TthHB8I