Restriction map for
pFN21AE4330
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseRI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstPI Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DdeI DraI
DrdI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HindIII KasI
KpnI KspI MamI MfeI MluI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 504 GACGTC GACGT^C
AccI 135 GTATAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AccIII 821 TCCGGA T^CCGGA
AcyI 504 GACGTC G[GA]^CG[CT]C
AfaI 217 GTAC GT^AC
AhdI 504 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 736 GTGCAC G^TGCAC
ApaLI 736 GTGCAC G^TGCAC
AspEI 504 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 455 TGGCCA TGG^CCA
BamHI 704 GGATCC G^GATCC
BanI 880 GGCACC G^G[CT][GA]CC
BbsI 501 GAAGAC GAAGAC(2/6)
BbvI 6 GCAGC GCAGC(8/12)
BclI 350 TGATCA T^GATCA
BfaI 199 CTAG C^TAG
BpmI 485 CTCCAG CTGGAG(16/14)
BpuAI 501 GAAGAC GAAGAC(2/6)
BsaHI 504 GACGTC G[GA]^CG[CT]C
BsaWI 821 TCCGGA [AT]^CCGG[AT]
BseAI 821 TCCGGA T^CCGGA
BspEI 821 TCCGGA T^CCGGA
BspMI 530 ACCTGC ACCTGC(4/8)
Bst1107I 135 GTATAC GTA^TAC
BstBI 318 TTCGAA TT^CGAA
BstUI 13 CGCG CG^CG
BstXI 789 CCACGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 135 GTATAC GTA^TAC
DraII 527 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 739 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 504 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 388 CTCTTC CTCTTC(1/4)
EcoNI 519 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 527 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 350 TGATCA T^GATCA
Hin1I 504 GACGTC G[GA]^CG[CT]C
HincII 685 GTTAAC GT[CT]^[GA]AC
HindII 685 GTTAAC GT[CT]^[GA]AC
HpaI 685 GTTAAC GTT^AAC
Ksp632I 388 CTCTTC CTCTTC(1/4)
MaeI 199 CTAG C^TAG
MluNI 455 TGGCCA TGG^CCA
MroI 821 TCCGGA T^CCGGA
MscI 455 TGGCCA TGG^CCA
MslI 867 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 13 CGCG CG^CG
NlaIII 459 CATG CATG^
NspV 318 TTCGAA TT^CGAA
PflMI 487 CCAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 527 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 217 GTAC GT^AC
SapI 387 GCTCTTC GCTCTTC(1/4)
SexAI 740 ACCTGGT A^CC[AT]GGT
SfaNI 223 GATGC GCATC(5/9)
SfiI 43 GGCCACCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 318 TTCGAA TT^CGAA
SmaI 731 CCCGGG CCC^GGG
SspI 448 AATATT AAT^ATT
TseI 6 GCAGC G^C[AT]GC
Van91I 487 CCAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 731 CCCGGG C^CCGGG
XmaI 731 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 175 AAATTT [GA]^AATT[CT]
928 AAATTC
ApoI 175 AAATTT [GA]^AATT[CT]
928 AAATTC
AvaI 409 CCCGAG C^[CT]CG[GA]G
731 CCCGGG
BglI 44 GCCACCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
574 GCCTGGACGGC
BsiEI 51 CGGCCG CG[GA][CT]^CG
62 CGGCCG
BsmFI 94 GGGAC GGGAC(10/14)
507 GTCCC
BsoBI 409 CCCGAG C^[CT]CG[GA]G
731 CCCGGG
BstYI 704 GGATCC [GA]^GATC[CT]
793 GGATCT
CfoI 14 GCGC GCG^C
572 GCGC
DsaI 699 CCACGG C^C[GA][CT]GG
789 CCACGG
EagI 51 CGGCCG C^GGCCG
62 CGGCCG
EclXI 51 CGGCCG C^GGCCG
62 CGGCCG
Eco52I 51 CGGCCG C^GGCCG
62 CGGCCG
HhaI 14 GCGC GCG^C
572 GCGC
HinP1I 14 GCGC G^CGC
572 GCGC
MaeII 505 ACGT A^CGT
872 ACGT
MaeIII 310 GTGAC ^GT[ACGT]AC
512 GTCAC
MflI 704 GGATCC [GA]^GATC[CT]
793 GGATCT
MseI 292 TTAA T^TAA
686 TTAA
PstI 28 CTGCAG CTGCA^G
264 CTGCAG
SacI 385 GAGCTC GAGCT^C
980 GAGCTC
SfcI 28 CTGCAG C^T[GA][CT]AG
264 CTGCAG
Tru9I 292 TTAA T^TAA
686 TTAA
Tsp45I 310 GTGAC ^GT[GC]AC
512 GTCAC
XhoII 704 GGATCC [GA]^GATC[CT]
793 GGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BcnI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TaqI TfiI
Tsp509I TspRI TthHB8I