(Circular) (Six-base) MAPSORT of: pFN21AE4330.seq Check: 7987 from: 1 to: 5719 pFN21AE4330 5719 bp With 182 enzymes: SgfI * March 28, 2012 14:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2513 3529 278 Size: 53 83 186 936 977 1016 2468 Fragments arranged by size: 2468 1016 977 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2141 3062 1937 Size: 204 921 4594 Fragments arranged by size: 4594 921 204 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2380 2536 2675 3782 5166 5434 1632 Size: 447 301 156 139 1107 1384 268 1917 Fragments arranged by size: 1917 1384 1107 447 301 268 156 139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 2514 4448 2514 Size: 1934 3785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2857 1184 Size: 1673 4046 AlwNI CAG_nnn'CTG Cuts at: 2403 2498 2536 3462 5034 2403 Size: 95 38 926 1572 3088 Fragments arranged by size: 3088 1572 926 95 38 ApaLI G'TGCA_C Cuts at: 2741 3776 4932 2741 Size: 1035 1156 3528 Fragments arranged by size: 3528 1156 1035 ApoI r'AATT_y Cuts at: 1493 2180 2933 3028 3254 3406 5515 1493 Size: 687 753 95 226 152 2109 1697 Fragments arranged by size: 2109 1697 753 687 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2414 2736 3044 1870 Size: 73 471 322 308 4545 Fragments arranged by size: 4545 471 322 308 73 BamHI G'GATC_C Cuts at: 2709 3049 3416 2709 Size: 340 367 5012 Fragments arranged by size: 5012 367 340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2885 3040 4495 618 Size: 325 217 627 1098 155 1455 1842 Fragments arranged by size: 1842 1455 1098 627 325 217 155 BanII G_rGCy'C Cuts at: 1391 2394 2651 2989 3038 1391 Size: 1003 257 338 49 4072 Fragments arranged by size: 4072 1003 338 257 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2513 928 Size: 971 614 4134 Fragments arranged by size: 4134 971 614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 2355 1548 Size: 807 4912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2055 2585 4330 Size: 107 122 71 631 605 383 530 1745 Cuts at: 4330 136 Size: 1525 Fragments arranged by size: 1745 1525 631 605 530 383 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2745 2888 3780 4936 1790 Size: 955 143 892 1156 2573 Fragments arranged by size: 2573 1156 955 892 143 BmgBI CAC'GTC Cuts at: 1318 2878 1318 Size: 1560 4159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3450 4408 442 Size: 416 53 83 399 24 2033 958 1753 Fragments arranged by size: 2033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 4379 1239 Size: 1236 1904 2579 Fragments arranged by size: 2579 1904 1236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 3526 3908 4543 Size: 53 83 186 936 977 1016 382 635 Cuts at: 4543 275 Size: 1451 Fragments arranged by size: 1451 1016 977 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2826 4152 4550 4824 4971 5382 2826 Size: 1326 398 274 147 411 3163 Fragments arranged by size: 3163 1326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2633 2663 1178 Size: 30 165 30 1230 30 4234 Fragments arranged by size: 4234 1230 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5719 BseYI C'CCAG_C Cuts at: 2558 4922 5418 2558 Size: 2364 496 2859 Fragments arranged by size: 2859 2364 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 2510 2522 1163 Size: 573 653 121 12 4360 Fragments arranged by size: 4360 653 573 121 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 2070 3169 3930 4958 1540 Size: 275 186 58 11 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 58 11 BsiHKAI G_wGCw'C Cuts at: 2394 2598 2631 2745 2989 3780 3865 4936 2394 Size: 204 33 114 244 791 85 1071 3177 Fragments arranged by size: 3177 1071 791 244 204 114 85 33 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2598 2631 2651 2745 2888 2989 Size: 399 604 204 33 20 94 143 101 Cuts at: 2989 3038 3780 3865 4936 1391 Size: 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 204 143 101 94 85 49 33 20 BspEI T'CCGG_A Cuts at: 2826 5382 2826 Size: 2556 3163 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2544 3074 844 Size: 1700 530 3489 Fragments arranged by size: 3489 1700 530 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 2324 4523 2324 Size: 2199 3520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2709 2798 3049 3416 3801 3818 5270 5356 Size: 737 89 251 367 385 17 1452 86 Cuts at: 5356 5368 5714 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 737 385 367 346 251 89 86 17 12 BstZ17I GTA'TAC Cuts at: 2142 2142 Size: 5719 BtgI C'CryG_G Cuts at: 513 1065 2004 2704 2794 513 Size: 552 939 700 90 3438 Fragments arranged by size: 3438 939 700 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2603 487 Size: 683 56 1377 3603 Fragments arranged by size: 3603 1377 683 56 BtsI GCAGTG_nn' Cuts at: 3235 4029 4049 3235 Size: 794 20 4905 Fragments arranged by size: 4905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DraIII CAC_nnn'GTG Cuts at: 2749 2749 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2067 2460 Size: 54 1425 50 275 18 226 11 393 Cuts at: 2460 3166 4055 5684 8 Size: 706 889 1629 43 Fragments arranged by size: 1629 1425 889 706 393 275 226 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2056 2067 3166 1537 Size: 275 244 11 1099 4090 Fragments arranged by size: 4090 1099 275 244 11 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 3649 1353 Size: 1046 1250 3423 Fragments arranged by size: 3423 1250 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 2987 2392 Size: 595 5124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2380 2475 2536 2675 3782 4379 Size: 393 447 301 95 61 139 1107 597 Cuts at: 4379 5166 5434 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1107 787 597 447 393 301 268 139 95 61 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5719 EcoO109I rG'GnC_Cy Cuts at: 2533 2533 Size: 5719 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 5719 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 4866 1678 Size: 3188 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2500 2532 4360 4392 4434 Size: 32 135 32 928 32 1828 32 42 Cuts at: 4434 4466 1373 Size: 32 2626 Fragments arranged by size: 2626 1828 928 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2692 3063 3309 669 Size: 988 281 754 371 246 3079 Fragments arranged by size: 3079 988 754 371 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 2692 3309 2692 Size: 617 5102 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 3267 Fragments arranged by size: 3267 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3677 4036 4195 518 Size: 648 1710 801 359 159 2042 Fragments arranged by size: 2042 1710 801 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3135 3812 4960 5205 1606 Size: 1529 677 1148 245 2120 Fragments arranged by size: 2120 1529 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4228 Fragments arranged by size: 4228 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 3077 4622 1132 Size: 97 1848 1545 2229 Fragments arranged by size: 2229 1848 1545 97 PasI CC'CwG_GG Cuts at: 1348 2818 1348 Size: 1470 4249 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 2498 1108 Size: 1390 4329 PfoI T'CCnGG_A Cuts at: 2367 2376 2712 2367 Size: 9 336 5374 Fragments arranged by size: 5374 336 9 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2533 2533 Size: 5719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PstI C_TGCA'G Cuts at: 830 2037 2273 3071 830 Size: 1207 236 798 3478 Fragments arranged by size: 3478 1207 798 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 SacI G_AGCT'C Cuts at: 2394 2989 2394 Size: 595 5124 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2399 2399 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SexAI A'CCwGG_T Cuts at: 2745 2745 Size: 5719 SfcI C'TryA_G Cuts at: 826 1045 2033 2269 3067 4202 4883 5074 826 Size: 219 988 236 798 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 798 681 236 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2055 2055 Size: 5719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 2738 3046 1872 Size: 866 308 4545 Fragments arranged by size: 4545 866 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 3823 4724 4986 820 Size: 197 926 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 2455 3643 5 Size: 47 2403 1188 2081 Fragments arranged by size: 2403 2081 1188 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2004 3158 513 Size: 552 939 1154 3074 Fragments arranged by size: 3074 1154 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3746 3763 3916 4101 4546 325 Size: 1255 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3965 5677 96 Size: 274 80 33 51 1443 1988 1712 138 Fragments arranged by size: 1988 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2633 2665 5679 5711 2633 Size: 32 3014 32 2641 Fragments arranged by size: 3014 2641 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 2736 3044 1870 Size: 866 308 4545 Fragments arranged by size: 4545 866 308 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2511 3527 276 Size: 53 83 186 936 977 1016 2468 Fragments arranged by size: 2468 1016 977 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfoI SrfI SwaI XcmI