(Linear) (Six-base) MAPSORT of: KIEE4330.seq Check: 1636 from: 1 to: 1014 KIEE4330.orf With 182 enzymes: SgfI * March 22, 2012 17:45 .. AatII G_ACGT'C Cuts at: 0 508 1014 Size: 508 506 AccI GT'mk_AC Cuts at: 0 136 1014 Size: 136 878 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 375 531 670 1014 Size: 74 301 156 139 344 Fragments arranged by size: 344 301 156 139 74 AhdI GACnn_n'nnGTC Cuts at: 0 509 1014 Size: 509 505 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 852 1014 Size: 852 162 AlwNI CAG_nnn'CTG Cuts at: 0 398 493 531 1014 Size: 398 95 38 483 Fragments arranged by size: 483 398 95 38 ApaLI G'TGCA_C Cuts at: 0 736 1014 Size: 736 278 ApoI r'AATT_y Cuts at: 0 175 928 1014 Size: 175 753 86 Fragments arranged by size: 753 175 86 AvaI C'yCGr_G Cuts at: 0 409 731 1014 Size: 409 322 283 Fragments arranged by size: 409 322 283 BamHI G'GATC_C Cuts at: 0 704 1014 Size: 704 310 BanI G'GyrC_C Cuts at: 0 880 1014 Size: 880 134 BanII G_rGCy'C Cuts at: 0 389 646 984 1014 Size: 389 257 338 30 Fragments arranged by size: 389 338 257 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 508 1014 Size: 508 506 BclI T'GATC_A Cuts at: 0 350 1014 Size: 350 664 BglI GCCn_nnn'nGGC Cuts at: 0 50 580 1014 Size: 50 530 434 Fragments arranged by size: 530 434 50 Bme1580I G_kGCm'C Cuts at: 0 740 883 1014 Size: 740 143 131 Fragments arranged by size: 740 143 131 BmgBI CAC'GTC Cuts at: 0 873 1014 Size: 873 141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1014 Size: 470 544 BsaHI Gr'CG_yC Cuts at: 0 505 1014 Size: 505 509 BsaWI w'CCGG_w Cuts at: 0 821 1014 Size: 821 193 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 628 658 1014 Size: 628 30 356 Fragments arranged by size: 628 356 30 BseYI C'CCAG_C Cuts at: 0 553 1014 Size: 553 461 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 505 517 1014 Size: 384 121 12 497 Fragments arranged by size: 497 384 121 12 BsiEI CG_ry'CG Cuts at: 0 54 65 1014 Size: 54 11 949 Fragments arranged by size: 949 54 11 BsiHKAI G_wGCw'C Cuts at: 0 389 593 626 740 984 1014 Size: 389 204 33 114 244 30 Fragments arranged by size: 389 244 204 114 33 30 Bsp1286I G_dGCh'C Cuts at: 0 389 593 626 646 740 883 984 1014 Size: 389 204 33 20 94 143 101 30 Fragments arranged by size: 389 204 143 101 94 33 30 20 BspEI T'CCGG_A Cuts at: 0 821 1014 Size: 821 193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 539 1014 Size: 539 475 BstBI TT'CG_AA Cuts at: 0 319 1014 Size: 319 695 BstXI CCAn_nnnn'nTGG Cuts at: 0 796 1014 Size: 796 218 BstYI r'GATC_y Cuts at: 0 704 793 1014 Size: 704 89 221 Fragments arranged by size: 704 221 89 BstZ17I GTA'TAC Cuts at: 0 137 1014 Size: 137 877 BtgI C'CryG_G Cuts at: 0 699 789 1014 Size: 699 90 225 Fragments arranged by size: 699 225 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 598 1014 Size: 598 416 DraIII CAC_nnn'GTG Cuts at: 0 744 1014 Size: 744 270 EaeI y'GGCC_r Cuts at: 0 51 62 455 1014 Size: 51 11 393 559 Fragments arranged by size: 559 393 51 11 EagI C'GGCC_G Cuts at: 0 51 62 1014 Size: 51 11 952 Fragments arranged by size: 952 51 11 EarI CTCTTCn'nnn_ Cuts at: 0 394 1014 Size: 394 620 EcoICRI GAG'CTC Cuts at: 0 387 982 1014 Size: 387 595 32 Fragments arranged by size: 595 387 32 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 375 470 531 670 1014 Size: 74 301 95 61 139 344 Fragments arranged by size: 344 301 139 95 74 61 EcoNI CCTnn'n_nnAGG Cuts at: 0 523 1014 Size: 523 491 EcoO109I rG'GnC_Cy Cuts at: 0 528 1014 Size: 528 486 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 405 1014 Size: 405 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 495 527 1014 Size: 495 32 487 Fragments arranged by size: 495 487 32 HincII GTy'rAC Cuts at: 0 687 1014 Size: 687 327 HpaI GTT'AAC Cuts at: 0 687 1014 Size: 687 327 MscI TGG'CCA Cuts at: 0 457 1014 Size: 457 557 MslI CAynn'nnrTG Cuts at: 0 871 1014 Size: 871 143 PasI CC'CwG_GG Cuts at: 0 813 1014 Size: 813 201 PflMI CCAn_nnn'nTGG Cuts at: 0 493 1014 Size: 493 521 PfoI T'CCnGG_A Cuts at: 0 362 371 707 1014 Size: 362 9 336 307 Fragments arranged by size: 362 336 307 9 PpuMI rG'GwC_Cy Cuts at: 0 528 1014 Size: 528 486 PstI C_TGCA'G Cuts at: 0 32 268 1014 Size: 32 236 746 Fragments arranged by size: 746 236 32 SacI G_AGCT'C Cuts at: 0 389 984 1014 Size: 389 595 30 Fragments arranged by size: 595 389 30 SapI GCTCTTCn'nnn_ Cuts at: 0 394 1014 Size: 394 620 SexAI A'CCwGG_T Cuts at: 0 740 1014 Size: 740 274 SfcI C'TryA_G Cuts at: 0 28 264 1014 Size: 28 236 750 Fragments arranged by size: 750 236 28 SfiI GGCCn_nnn'nGGCC Cuts at: 0 50 1014 Size: 50 964 SmaI CCC'GGG Cuts at: 0 733 1014 Size: 733 281 SspI AAT'ATT Cuts at: 0 450 1014 Size: 450 564 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 628 660 1014 Size: 628 32 354 Fragments arranged by size: 628 354 32 XmaI C'CCGG_G Cuts at: 0 731 1014 Size: 731 283 ZraI GAC'GTC Cuts at: 0 506 1014 Size: 506 508 Enzymes that do cut: AatII AccI AcuI AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI BglI Bme1580I BmgBI BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BstBI BstXI BstYI BstZ17I BtgI BtgZI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HpaI MscI MslI PasI PflMI PfoI PpuMI PstI SacI SapI SexAI SfcI SfiI SmaI SspI TstI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII Bsu36I BtsI ClaI CspCI DraI DrdI EciI EcoRI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI