################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MluI MluNI MroI MscI MslI NarI NciI NcoI NheI NotI NruI NsiI NspI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AfaI 980 GTAC GT^AC AlwI 607 GATCC GGATC(4/5) AlwNI 124 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 155 GGGCCC GGGCC^C Asp700I 897 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbvCI 17 CCTCAGC CCTCAGC(-5/-2) BciVI 873 GGATAC GTATCC(6/5) BglI 32 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1114 AGATCT A^GATCT BsaI 887 GGTCTC GGTCTC(1/5) BsaWI 1181 ACCGGA [AT]^CCGG[AT] BsgI 145 GTGCAG GTGCAG(16/14) BsiEI 276 CGGCCG CG[GA][CT]^CG BsmAI 888 GTCTC GTCTC(1/5) BsmI 118 GAATGC GAATGC(1/-1) BspHI 504 TCATGA T^CATGA BspMI 147 GCAGGT ACCTGC(4/8) BsrBI 279 CCGCTC CCGCTC(-3/-3) BstBI 115 TTCGAA TT^CGAA BstYI 1114 AGATCT [GA]^GATC[CT] CfoI 311 GCGC GCG^C DraI 568 TTTAAA TTT^AAA DrdI 332 GACTATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 276 CGGCCG C^GGCCG EclXI 276 CGGCCG C^GGCCG Eco52I 276 CGGCCG C^GGCCG EcoT14I 449 CCTTGG C^C[AT][AT]GG HgaI 187 GACGC GACGC(5/10) HhaI 311 GCGC GCG^C HinP1I 311 GCGC G^CGC HphI 604 GGTGA GGTGA(8/7) KspI 34 CCGCGG CCGC^GG MaeIII 1048 GTAAC ^GT[ACGT]AC MfeI 479 CAATTG C^AATTG MflI 1114 AGATCT [GA]^GATC[CT] MunI 479 CAATTG C^AATTG NaeI 223 GCCGGC GCC^GGC NgoMIV 223 GCCGGC G^CCGGC NspV 115 TTCGAA TT^CGAA PacI 523 TTAATTAA TTAAT^TAA PaeR7I 133 CTCGAG C^TCGAG PflMI 702 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1038 GAGTC GAGTC(4/5) PpuMI 298 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1185 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 504 TCATGA T^CATGA RsaI 980 GTAC GT^AC SacII 34 CCGCGG CCGC^GG SapI 1004 GAAGAGC GCTCTTC(1/4) SfcI 539 CTATAG C^T[GA][CT]AG SfuI 115 TTCGAA TT^CGAA SspI 597 AATATT AAT^ATT StyI 449 CCTTGG C^C[AT][AT]GG TspRI 1011 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 702 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 133 CTCGAG C^TCGAG XhoII 1114 AGATCT [GA]^GATC[CT] XmnI 897 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 299 GGACC G^G[AT]CC 1214 GGACC BanII 30 GAGCCC G[GA]GC[CT]^C 155 GGGCCC BbsI 1099 GAAGAC GAAGAC(2/6) 1252 GAAGAC BfaI 833 CTAG C^TAG 1070 CTAG BmyI 30 GAGCCC G[AGT]GC[ACT]^C 155 GGGCCC BpuAI 1099 GAAGAC GAAGAC(2/6) 1252 GAAGAC Bsp1286I 30 GAGCCC G[AGT]GC[ACT]^C 155 GGGCCC BsrFI 223 GCCGGC [GA]^CCGG[CT] 1044 GCCGGT Cfr10I 223 GCCGGC [GA]^CCGG[CT] 1044 GCCGGT DpnII 607 GATC ^GATC 1115 GATC DsaI 34 CCGCGG C^C[GA][CT]GG 215 CCGTGG EaeI 200 TGGCCG [CT]^GGCC[GA] 276 CGGCCG EarI 959 GAAGAG CTCTTC(1/4) 1004 GAAGAG Ksp632I 959 GAAGAG CTCTTC(1/4) 1004 GAAGAG MaeI 833 CTAG C^TAG 1070 CTAG MboI 607 GATC ^GATC 1115 GATC NdeI 372 CATATG CA^TATG 619 CATATG NdeII 607 GATC ^GATC 1115 GATC Sau3AI 607 GATC ^GATC 1115 GATC SmlI 133 CTCGAG C^T[CT][GA]AG 821 CTCAAG TfiI 454 GAATC G^A[AT]TC 897 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AcsI AluI ApoI AvaI BbvI BsaJI BseRI BsiYI BslI BsoBI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TthHB8I