################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 99 CGCG CG^CG AluI 79 AGCT AG^CT AocI 222 CCTCAGG CC^T[ACGT]AGG AspHI 274 GAGCAC G[AT]GC[AT]^C BbsI 388 GTCTTC GAAGAC(2/6) BcnI 312 CCGGG CC^[GC]GG BlpI 162 GCTCAGC GC^T[ACGT]AGC BmyI 274 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC BpuAI 388 GTCTTC GAAGAC(2/6) BsiHKAI 274 GAGCAC G[AT]GC[AT]^C BsmFI 304 GTCCC GGGAC(10/14) Bsp1286I 274 GAGCAC G[AGT]GC[ACT]^C BstAPI 364 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 369 GGATG GGATG(2/0) BstUI 99 CGCG CG^CG Bsu36I 222 CCTCAGG CC^T[ACGT]AGG CelII 162 GCTCAGC GC^T[ACGT]AGC CfoI 100 GCGC GCG^C DraIII 277 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 222 CCTCAGG CC^T[ACGT]AGG FokI 369 GGATG GGATG(9/13) HgaI 159 GACGC GACGC(5/10) HhaI 100 GCGC GCG^C HinP1I 100 GCGC G^CGC HphI 153 GGTGA GGTGA(8/7) MaeIII 259 GTAAC ^GT[ACGT]AC MslI 147 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 99 CGCG CG^CG NciI 312 CCGGG CC^[GC]GG NlaIV 303 GGTCCC GG[ACGT]^[ACGT]CC SexAI 278 ACCTGGT A^CC[AT]GGT TaqI 128 TCGA T^CGA TthHB8I 128 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 27 CCGC CCGC(-3/-1) 90 GCGG AvaII 219 GGACC G^G[AT]CC 303 GGTCC BbvI 102 GCTGC GCAGC(8/12) 105 GCTGC BpmI 318 CTGGAG CTGGAG(16/14) 331 CTGGAG BsgI 187 GTGCAG GTGCAG(16/14) 289 GTGCAG BsmAI 321 GAGAC GTCTC(1/5) 34 GTCTC BspMI 59 ACCTGC ACCTGC(4/8) 384 GCAGGT BsrI 16 ACTGG ACTGG(1/-1) 326 ACTGG Cac8I 63 GCAAGC GC[ACGT]^[ACGT]GC 380 GCGAGC Cfr13I 219 GGACC G^G[ACGT]CC 303 GGTCC DdeI 163 CTCAG C^T[ACGT]AG 223 CTCAG DpnII 96 GATC ^GATC 126 GATC DraII 218 AGGACCT [GA]G^G[ACGT]CC[CT] 302 AGGTCCC EcoO109I 218 AGGACCT [GA]G^G[ACGT]CC[CT] 302 AGGTCCC Fnu4HI 102 GCTGC GC^[ACGT]GC 105 GCTGC HapII 115 CCGG C^CGG 312 CCGG HpaII 115 CCGG C^CGG 312 CCGG ItaI 102 GCTGC GC^[ACGT]GC 105 GCTGC MboI 96 GATC ^GATC 126 GATC MspI 115 CCGG C^CGG 312 CCGG NdeII 96 GATC ^GATC 126 GATC NlaIII 48 CATG CATG^ 147 CATG PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT] 302 AGGTCCC Sau3AI 96 GATC ^GATC 126 GATC Sau96I 219 GGACC G^G[ACGT]CC 303 GGTCC SfaNI 342 GATGC GCATC(5/9) 370 GATGC TseI 102 GCTGC G^C[AT]GC 105 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### BsaJI BseRI BsiYI BslI BssKI BstNI EcoRII MboII MnlI MvaI MwoI ScrFI TspRI