(Circular) (Six-base) MAPSORT of: pFN21AE4552.seq Check: 8423 from: 1 to: 6691 pFN21AE4552 6691 bp With 182 enzymes: SgfI * March 28, 2012 14:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3237 3634 3237 Size: 397 6294 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3533 4501 278 Size: 53 83 186 936 1997 968 2468 Fragments arranged by size: 2468 1997 968 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2534 2564 3129 3461 4754 6138 6406 Size: 642 260 30 565 332 1293 1384 268 Cuts at: 6406 1632 Size: 1917 Fragments arranged by size: 1917 1384 1293 642 565 332 268 260 30 AfeI AGC'GCT Cuts at: 2186 2601 2186 Size: 415 6276 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 3121 5420 3121 Size: 2299 4392 AleI CACnn'nnGTG Cuts at: 1166 3656 1166 Size: 2490 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2921 1184 Size: 1737 4954 AlwNI CAG_nnn'CTG Cuts at: 3607 4434 6006 3607 Size: 827 1572 4292 Fragments arranged by size: 4292 1572 827 ApaI G_GGCC'C Cuts at: 3665 3665 Size: 6691 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2225 2301 4000 4226 4378 6487 1493 Size: 732 76 1699 226 152 2109 1697 Fragments arranged by size: 2109 1699 1697 732 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2439 3062 3694 3920 4016 1870 Size: 73 496 623 632 226 96 4545 Fragments arranged by size: 4545 632 623 496 226 96 73 BamHI G'GATC_C Cuts at: 4021 4388 4021 Size: 367 6324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 3096 3578 4012 5467 Size: 325 217 627 237 1072 482 434 1455 Cuts at: 5467 618 Size: 1842 Fragments arranged by size: 1842 1455 1072 627 482 434 325 237 217 BanII G_rGCy'C Cuts at: 1391 2070 2721 2778 3665 4010 1391 Size: 679 651 57 887 345 4072 Fragments arranged by size: 4072 887 679 651 345 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5720 BbvCI CC'TCA_GC Cuts at: 3197 3197 Size: 6691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4882 4916 1903 Size: 34 2945 34 3678 Fragments arranged by size: 3678 2945 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2273 2384 4583 5793 2273 Size: 111 2199 1210 3171 Fragments arranged by size: 3171 2199 1210 111 BclI T'GATC_A Cuts at: 1548 2995 1548 Size: 1447 5244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3814 6686 3814 Size: 2872 3819 BlpI GC'TnA_GC Cuts at: 4108 4108 Size: 6691 Bme1580I G_kGCm'C Cuts at: 1790 3480 3581 3665 4752 5908 1790 Size: 1690 101 84 1087 1156 2573 Fragments arranged by size: 2573 1690 1156 1087 101 84 BmgBI CAC'GTC Cuts at: 1318 3475 3636 3840 3883 1318 Size: 2157 161 204 43 4126 Fragments arranged by size: 4126 2157 204 161 43 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3491 3659 4422 Size: 416 53 83 399 24 2074 168 763 Cuts at: 4422 5380 442 Size: 958 1753 Fragments arranged by size: 2074 1753 958 763 416 399 168 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3136 3136 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2682 2699 3413 5351 1239 Size: 1443 17 714 1938 2579 Fragments arranged by size: 2579 1938 1443 714 17 Bpu10I CC'TnA_GC Cuts at: 3197 3197 Size: 6691 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2766 3331 4816 5681 5979 2766 Size: 565 1485 865 298 3478 Fragments arranged by size: 3478 1485 865 565 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2764 3051 5354 882 Size: 617 1265 287 2303 2219 Fragments arranged by size: 2303 2219 1265 617 287 BsaAI yAC'GTr Cuts at: 493 1204 3729 4482 493 Size: 711 2525 753 2702 Fragments arranged by size: 2702 2525 753 711 BsaBI GATnn'nnATC Cuts at: 2378 6685 2378 Size: 4307 2384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3530 3546 4498 4880 Size: 53 83 186 936 1997 16 952 382 Cuts at: 4880 5515 275 Size: 635 1451 Fragments arranged by size: 1997 1451 952 936 635 382 186 83 53 16 BsaWI w'CCGG_w Cuts at: 2457 5124 5522 5796 5943 6354 2457 Size: 2667 398 274 147 411 2794 Fragments arranged by size: 2794 2667 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 2726 2756 3020 Size: 30 165 30 654 30 639 30 264 Cuts at: 3020 3050 1178 Size: 30 4819 Fragments arranged by size: 4819 654 639 264 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 3109 3251 1186 Size: 996 927 142 4626 Fragments arranged by size: 4626 996 927 142 BseYI C'CCAG_C Cuts at: 2181 2602 2819 2953 3574 5894 6390 2181 Size: 421 217 134 621 2320 496 2482 Fragments arranged by size: 2482 2320 621 496 421 217 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 3803 1163 Size: 573 798 1269 4051 Fragments arranged by size: 4051 1269 798 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 4141 4902 5930 1540 Size: 275 186 21 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 3687 4752 4837 5908 3687 Size: 1065 85 1071 4470 Fragments arranged by size: 4470 1071 1065 85 BsmI GAATG_Cn' Cuts at: 2041 4202 4295 2041 Size: 2161 93 4437 Fragments arranged by size: 4437 2161 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2120 2393 3830 2120 Size: 273 1437 4981 Fragments arranged by size: 4981 1437 273 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2721 2778 3480 3581 3665 3687 Size: 399 280 651 57 702 101 84 22 Cuts at: 3687 4010 4752 4837 5908 1391 Size: 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 651 399 323 280 101 85 84 57 22 BspEI T'CCGG_A Cuts at: 2457 6354 2457 Size: 3897 2794 BspHI T'CATG_A Cuts at: 3204 4580 6310 3204 Size: 1376 1730 3585 Fragments arranged by size: 3585 1730 1376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 2454 3237 3634 4046 844 Size: 1298 312 783 397 412 3489 Fragments arranged by size: 3489 1298 783 412 397 312 BsrBI CCG'CTC Cuts at: 1394 1823 3020 4578 1394 Size: 429 1197 1558 3507 Fragments arranged by size: 3507 1558 1197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3948 5186 5360 65 Size: 1183 2700 1238 174 1396 Fragments arranged by size: 2700 1396 1238 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3099 5335 5522 1700 Size: 110 1289 2236 187 2869 Fragments arranged by size: 2869 2236 1289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 4751 5763 4751 Size: 1012 5679 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6691 BstBI TT'CG_AA Cuts at: 3774 5495 3774 Size: 1721 4970 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2677 2731 3466 3658 2113 Size: 564 54 735 192 5146 Fragments arranged by size: 5146 735 564 192 54 BstYI r'GATC_y Cuts at: 1972 3814 4021 4388 4773 4790 6242 6328 6340 Size: 1842 207 367 385 17 1452 86 12 Cuts at: 6340 6686 1972 Size: 346 1977 Fragments arranged by size: 1977 1842 1452 385 367 346 207 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3965 3965 Size: 6691 BtgI C'CryG_G Cuts at: 513 1065 2004 3126 3465 513 Size: 552 939 1122 339 3739 Fragments arranged by size: 3739 1122 939 552 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2656 487 Size: 683 56 1430 4522 Fragments arranged by size: 4522 1430 683 56 BtsI GCAGTG_nn' Cuts at: 4207 5001 5021 4207 Size: 794 20 5877 Fragments arranged by size: 5877 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2334 2667 Size: 54 1425 50 275 18 177 327 333 Cuts at: 2667 3456 3648 4138 5027 6656 8 Size: 789 192 490 889 1629 43 Fragments arranged by size: 1629 1425 889 789 490 333 327 275 192 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3105 3185 3335 4621 1353 Size: 1484 268 80 150 1286 3423 Fragments arranged by size: 3423 1484 1286 268 150 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3932 5278 5652 5798 387 Size: 1064 138 2343 1346 374 146 1280 Fragments arranged by size: 2343 1346 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2534 2564 2682 2699 3129 3413 Size: 393 642 260 30 118 17 430 284 Cuts at: 3413 3461 4754 5351 6138 6406 1239 Size: 48 1293 597 787 268 1524 Fragments arranged by size: 1524 1293 787 642 597 430 393 284 268 260 118 48 30 17 EcoNI CCTnn'n_nnAGG Cuts at: 2266 3760 2266 Size: 1494 5197 EcoO109I rG'GnC_Cy Cuts at: 2233 2258 2345 2443 3189 2233 Size: 25 87 98 746 5735 Fragments arranged by size: 5735 746 98 87 25 EcoRI G'AATT_C Cuts at: 2225 4000 4378 2225 Size: 1775 378 4538 Fragments arranged by size: 4538 1775 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3208 3325 3208 Size: 117 6574 FspI TGC'GCA Cuts at: 3233 5197 3233 Size: 1964 4727 HaeII r_GCGC'y Cuts at: 1678 2188 2603 2665 2742 2826 5838 1678 Size: 510 415 62 77 84 3012 2531 Fragments arranged by size: 3012 2531 510 415 84 77 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2087 2089 2119 2726 Size: 32 135 32 485 30 2 30 607 Cuts at: 2726 2758 2963 2995 3107 3139 5332 5364 5406 Size: 32 205 32 112 32 2193 32 42 Cuts at: 5406 5438 1373 Size: 32 2626 Fragments arranged by size: 2626 2193 607 485 205 135 112 42 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 4035 4281 669 Size: 988 281 2097 246 3079 Fragments arranged by size: 3079 2097 988 281 246 HindIII A'AGCT_T Cuts at: 748 2546 748 Size: 1798 4893 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3567 3837 5805 5989 1305 Size: 2262 270 1968 184 2007 Fragments arranged by size: 2262 2007 1968 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 2669 3458 3650 10 Size: 54 1425 343 504 333 789 192 3051 Fragments arranged by size: 3051 1425 789 504 343 333 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2372 2528 3656 3680 4649 5008 Size: 648 864 342 156 1128 24 969 359 Cuts at: 5008 5167 518 Size: 159 2042 Fragments arranged by size: 2042 1128 969 864 648 359 342 159 156 24 MspA1I CmG'CkG Cuts at: 1606 2213 2727 3156 4107 4784 5932 6177 1606 Size: 607 514 429 951 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 607 514 429 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3126 3465 513 Size: 552 939 1122 339 3739 Fragments arranged by size: 3739 1122 939 552 339 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2494 3037 4049 5594 1132 Size: 97 1265 543 1012 1545 2229 Fragments arranged by size: 2229 1545 1265 1012 543 97 PasI CC'CwG_GG Cuts at: 1348 3607 1348 Size: 2259 4432 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6691 PfoI T'CCnGG_A Cuts at: 2306 2306 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2258 2345 2443 3189 2233 Size: 25 87 98 746 5735 Fragments arranged by size: 5735 746 98 87 25 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 4261 4261 Size: 6691 PspOMI G'GGCC_C Cuts at: 3661 3661 Size: 6691 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3416 3448 3416 Size: 32 6659 PstI C_TGCA'G Cuts at: 830 2056 3512 3645 4043 830 Size: 1226 1456 133 398 3478 Fragments arranged by size: 3478 1456 1226 398 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2213 3156 2213 Size: 943 5748 RsrII CG'GwC_CG Cuts at: 2631 2631 Size: 6691 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SbfI CC_TGCA'GG Cuts at: 3645 4043 3645 Size: 398 6293 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SfcI C'TryA_G Cuts at: 826 1045 2052 3508 3641 3734 4039 5174 5855 Size: 219 1007 1456 133 93 305 1135 681 Cuts at: 5855 6046 826 Size: 191 1471 Fragments arranged by size: 1471 1456 1135 1007 681 305 219 191 133 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 2441 4018 1872 Size: 569 1577 4545 Fragments arranged by size: 4545 1577 569 SmlI C'TyrA_G Cuts at: 820 1017 1943 2781 3310 4795 5696 5958 820 Size: 197 926 838 529 1485 901 262 1553 Fragments arranged by size: 1553 1485 926 901 838 529 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 4615 5 Size: 47 4563 2081 Fragments arranged by size: 4563 2081 47 StuI AGG'CCT Cuts at: 1517 3446 1517 Size: 1929 4762 StyI C'CwwG_G Cuts at: 513 1065 2004 3126 3465 4130 513 Size: 552 939 1122 339 665 3074 Fragments arranged by size: 3074 1122 939 665 552 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 3205 4718 4735 4888 5073 5518 Size: 1255 428 1197 1513 17 153 185 445 Cuts at: 5518 325 Size: 1498 Fragments arranged by size: 1513 1498 1255 1197 445 428 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3276 3519 4937 Size: 274 80 33 51 1443 1299 243 1418 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1418 1299 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2862 2894 3020 3052 6651 6683 2862 Size: 32 126 32 3599 32 2870 Fragments arranged by size: 3599 2870 126 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3215 1316 Size: 1899 4792 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2728 3589 3901 2728 Size: 861 312 5518 Fragments arranged by size: 5518 861 312 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 2439 4016 1870 Size: 569 1577 4545 Fragments arranged by size: 4545 1577 569 XmnI GAAnn'nnTTC Cuts at: 4820 4820 Size: 6691 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3531 4499 276 Size: 53 83 186 936 1997 968 2468 Fragments arranged by size: 2468 1997 968 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI