(Circular) (Six-base) MAPSORT of: pFN21AE4403.seq Check: 9810 from: 1 to: 5962 pFN21AE4403 5962 bp With 182 enzymes: SgfI * March 28, 2012 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 2091 3283 2091 Size: 1192 4770 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2714 4861 5838 1203 Size: 1511 2147 977 1327 Fragments arranged by size: 2147 1511 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 2344 4691 2344 Size: 2347 3615 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2880 2912 2880 Size: 32 5930 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2493 2547 3287 1870 Size: 73 550 54 740 4545 Fragments arranged by size: 4545 740 550 73 54 AvrII C'CTAG_G Cuts at: 2138 2935 2138 Size: 797 5165 BamHI G'GATC_C Cuts at: 3000 3292 3659 3000 Size: 292 367 5303 Fragments arranged by size: 5303 367 292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2091 3229 3283 4738 Size: 325 217 627 282 22 1138 54 1455 Cuts at: 4738 618 Size: 1842 Fragments arranged by size: 1842 1455 1138 627 325 282 217 54 22 BanII G_rGCy'C Cuts at: 1391 3281 1391 Size: 1890 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 928 Size: 971 582 4409 Fragments arranged by size: 4409 971 582 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2933 3854 5064 2933 Size: 921 1210 3831 Fragments arranged by size: 3831 1210 921 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2444 2444 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2525 3011 3379 2525 Size: 486 368 5108 Fragments arranged by size: 5108 486 368 Bme1580I G_kGCm'C Cuts at: 1790 2072 4023 5179 1790 Size: 282 1951 1156 2573 Fragments arranged by size: 2573 1951 1156 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2550 2550 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2304 2520 2612 3041 4622 1239 Size: 1065 216 92 429 1581 2579 Fragments arranged by size: 2579 1581 1065 429 216 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2585 4087 4952 5250 2585 Size: 1502 865 298 3297 Fragments arranged by size: 3297 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2482 3753 493 Size: 711 1278 1271 2702 Fragments arranged by size: 2702 1278 1271 711 BsaBI GATnn'nnATC Cuts at: 3164 5956 3164 Size: 2792 3170 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2906 2936 1178 Size: 30 165 30 1503 30 4204 Fragments arranged by size: 4204 1503 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 2813 1186 Size: 1171 456 4335 Fragments arranged by size: 4335 1171 456 BseYI C'CCAG_C Cuts at: 2720 2910 5165 5661 2720 Size: 190 2255 496 3021 Fragments arranged by size: 3021 2255 496 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2527 1163 Size: 573 791 4598 Fragments arranged by size: 4598 791 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3096 3412 4173 5201 1540 Size: 275 186 1095 316 761 1028 2301 Fragments arranged by size: 2301 1095 1028 761 316 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 3281 4023 4108 5179 1391 Size: 399 282 1209 742 85 1071 2174 Fragments arranged by size: 2174 1209 1071 742 399 282 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2181 3317 844 Size: 1259 78 1136 3489 Fragments arranged by size: 3489 1259 1136 78 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2047 4457 4631 65 Size: 1183 799 2410 174 1396 Fragments arranged by size: 2410 1396 1183 799 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 4606 4793 1700 Size: 110 293 2503 187 2869 Fragments arranged by size: 2869 2503 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2482 794 Size: 1688 4274 BstBI TT'CG_AA Cuts at: 2960 4766 2960 Size: 1806 4156 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 3065 3065 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2434 3000 3036 3292 3659 4044 4061 5513 Size: 462 566 36 256 367 385 17 1452 Cuts at: 5513 5599 5611 5957 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 566 462 385 367 346 256 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2518 3090 513 Size: 552 939 60 454 572 3385 Fragments arranged by size: 3385 939 572 552 454 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2583 3084 3478 4272 4292 2583 Size: 501 394 794 20 4253 Fragments arranged by size: 4253 794 501 394 20 ClaI AT'CG_AT Cuts at: 1554 2670 3168 1554 Size: 1116 498 4348 Fragments arranged by size: 4348 1116 498 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2035 2196 Size: 54 1425 50 275 18 177 28 161 Cuts at: 2196 2299 2506 2577 2617 2738 3409 4298 5927 Size: 103 207 71 40 121 671 889 1629 Cuts at: 5927 8 Size: 43 Fragments arranged by size: 1629 1425 889 671 275 207 177 161 121 103 71 54 50 43 40 28 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2520 2612 3041 4025 4622 5409 Size: 393 672 216 92 429 984 597 787 Cuts at: 5409 5677 1239 Size: 268 1524 Fragments arranged by size: 1524 984 787 672 597 429 393 268 216 92 EcoO109I rG'GnC_Cy Cuts at: 2596 2608 3019 2596 Size: 12 411 5539 Fragments arranged by size: 5539 411 12 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 2177 2501 4468 2177 Size: 324 1967 3671 Fragments arranged by size: 3671 1967 324 FspAI rTGC'GCAy Cuts at: 2501 2501 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2935 5109 1678 Size: 1257 2174 2531 Fragments arranged by size: 2531 2174 1257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2973 3005 4603 Size: 32 135 32 758 32 611 32 1598 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1598 758 611 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 2228 748 Size: 1480 4482 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 2095 3287 2095 Size: 1192 4770 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3141 3186 5076 5260 1305 Size: 1836 45 1890 184 2007 Fragments arranged by size: 2007 1890 1836 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2301 2579 2740 10 Size: 54 1425 343 177 292 278 161 3232 Fragments arranged by size: 3232 1425 343 292 278 177 161 54 MslI CAynn'nnrTG Cuts at: 518 1166 2766 3920 4279 4438 518 Size: 648 1600 1154 359 159 2042 Fragments arranged by size: 2042 1600 1154 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2662 3092 3378 4055 5203 5448 1606 Size: 1056 430 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 1056 677 430 286 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2064 513 Size: 552 939 60 4411 Fragments arranged by size: 4411 939 552 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 2446 2446 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2718 3320 4865 1132 Size: 97 1489 602 1545 2229 Fragments arranged by size: 2229 1545 1489 602 97 PasI CC'CwG_GG Cuts at: 1348 2412 1348 Size: 1064 4898 PciI A'CATG_T Cuts at: 2714 4861 2714 Size: 2147 3815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PfoI T'CCnGG_A Cuts at: 2127 3039 2127 Size: 912 5050 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 3019 2608 Size: 411 5551 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SacII CC_GC'GG Cuts at: 3093 3093 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SexAI A'CCwGG_T Cuts at: 3183 3183 Size: 5962 SfcI C'TryA_G Cuts at: 826 1045 3310 4445 5126 5317 826 Size: 219 2265 1135 681 191 1471 Fragments arranged by size: 2265 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2549 3289 1872 Size: 677 740 4545 Fragments arranged by size: 4545 740 677 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 4066 4967 5229 820 Size: 197 926 657 1466 901 262 1553 Fragments arranged by size: 1553 1466 926 901 657 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2138 2458 2935 2965 3401 Size: 552 939 60 74 320 477 30 436 Cuts at: 3401 513 Size: 3074 Fragments arranged by size: 3074 939 552 477 436 320 74 60 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2263 2471 3989 4006 4159 4344 4789 Size: 1255 683 208 1518 17 153 185 445 Cuts at: 4789 325 Size: 1498 Fragments arranged by size: 1518 1498 1255 683 445 208 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2716 2748 2906 2938 5922 5954 2716 Size: 32 158 32 2984 32 2724 Fragments arranged by size: 2984 2724 158 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2378 1316 Size: 1062 4900 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2587 2587 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2547 3287 1870 Size: 677 740 4545 Fragments arranged by size: 4545 740 677 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI