(Linear) (Six-base) MAPSORT of: KIEE4403.seq Check: 1576 from: 1 to: 1257 KIEE4403.orf With 182 enzymes: SgfI * March 22, 2012 17:55 .. Acc65I G'GTAC_C Cuts at: 0 86 1257 Size: 86 1171 AflIII A'CryG_T Cuts at: 0 709 1257 Size: 709 548 AhdI GACnn_n'nnGTC Cuts at: 0 339 1257 Size: 339 918 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 875 907 1257 Size: 875 32 350 Fragments arranged by size: 875 350 32 AvaI C'yCGr_G Cuts at: 0 488 542 1257 Size: 488 54 715 Fragments arranged by size: 715 488 54 AvrII C'CTAG_G Cuts at: 0 133 930 1257 Size: 133 797 327 Fragments arranged by size: 797 327 133 BamHI G'GATC_C Cuts at: 0 995 1257 Size: 995 262 BanI G'GyrC_C Cuts at: 0 64 86 1224 1257 Size: 64 22 1138 33 Fragments arranged by size: 1138 64 33 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 476 1257 Size: 476 781 BciVI GTATCCnnnnn_n' Cuts at: 0 928 1257 Size: 928 329 BfrBI ATG'CAT Cuts at: 0 439 1257 Size: 439 818 BlpI GC'TnA_GC Cuts at: 0 520 1006 1257 Size: 520 486 251 Fragments arranged by size: 520 486 251 Bme1580I G_kGCm'C Cuts at: 0 67 1257 Size: 67 1190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 545 1257 Size: 545 712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 299 515 607 1036 1257 Size: 299 216 92 429 221 Fragments arranged by size: 429 299 221 216 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 1257 Size: 580 677 BsaAI yAC'GTr Cuts at: 0 477 1257 Size: 477 780 BsaBI GATnn'nnATC Cuts at: 0 1159 1257 Size: 1159 98 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 901 931 1257 Size: 901 30 326 Fragments arranged by size: 901 326 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 352 808 1257 Size: 352 456 449 Fragments arranged by size: 456 449 352 BseYI C'CCAG_C Cuts at: 0 715 905 1257 Size: 715 190 352 Fragments arranged by size: 715 352 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 1257 Size: 522 735 BsiEI CG_ry'CG Cuts at: 0 1091 1257 Size: 1091 166 Bsp1286I G_dGCh'C Cuts at: 0 67 1257 Size: 67 1190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 98 176 1257 Size: 98 78 1081 Fragments arranged by size: 1081 98 78 BsrDI GCAATG_nn' Cuts at: 0 42 1257 Size: 42 1215 BsrFI r'CCGG_y Cuts at: 0 98 1257 Size: 98 1159 BstAPI GCAn_nnn'nTGC Cuts at: 0 477 1257 Size: 477 780 BstBI TT'CG_AA Cuts at: 0 955 1257 Size: 955 302 BstXI CCAn_nnnn'nTGG Cuts at: 0 1060 1257 Size: 1060 197 BstYI r'GATC_y Cuts at: 0 429 995 1031 1257 Size: 429 566 36 226 Fragments arranged by size: 566 429 226 36 BtgI C'CryG_G Cuts at: 0 59 513 1085 1257 Size: 59 454 572 172 Fragments arranged by size: 572 454 172 59 BtsI GCAGTG_nn' Cuts at: 0 578 1079 1257 Size: 578 501 178 Fragments arranged by size: 578 501 178 ClaI AT'CG_AT Cuts at: 0 665 1163 1257 Size: 665 498 94 Fragments arranged by size: 665 498 94 EaeI y'GGCC_r Cuts at: 0 2 30 191 294 501 572 612 733 Size: 2 28 161 103 207 71 40 121 Cuts at: 733 1257 Size: 524 Fragments arranged by size: 524 207 161 121 103 71 40 28 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 299 515 607 1036 1257 Size: 299 216 92 429 221 Fragments arranged by size: 429 299 221 216 92 EcoO109I rG'GnC_Cy Cuts at: 0 591 603 1014 1257 Size: 591 12 411 243 Fragments arranged by size: 591 411 243 12 FspI TGC'GCA Cuts at: 0 172 496 1257 Size: 172 324 761 Fragments arranged by size: 761 324 172 FspAI rTGC'GCAy Cuts at: 0 496 1257 Size: 496 761 HaeII r_GCGC'y Cuts at: 0 930 1257 Size: 930 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 325 357 968 1000 1257 Size: 325 32 611 32 257 Fragments arranged by size: 611 325 257 32 32 HindIII A'AGCT_T Cuts at: 0 223 1257 Size: 223 1034 KpnI G_GTAC'C Cuts at: 0 90 1257 Size: 90 1167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1136 1181 1257 Size: 1136 45 76 Fragments arranged by size: 1136 76 45 MscI TGG'CCA Cuts at: 0 4 296 574 735 1257 Size: 4 292 278 161 522 Fragments arranged by size: 522 292 278 161 4 MslI CAynn'nnrTG Cuts at: 0 761 1257 Size: 761 496 MspA1I CmG'CkG Cuts at: 0 657 1087 1257 Size: 657 430 170 Fragments arranged by size: 657 430 170 NcoI C'CATG_G Cuts at: 0 59 1257 Size: 59 1198 NsiI A_TGCA'T Cuts at: 0 441 1257 Size: 441 816 NspI r_CATG'y Cuts at: 0 713 1257 Size: 713 544 PasI CC'CwG_GG Cuts at: 0 407 1257 Size: 407 850 PciI A'CATG_T Cuts at: 0 709 1257 Size: 709 548 PfoI T'CCnGG_A Cuts at: 0 122 1034 1257 Size: 122 912 223 Fragments arranged by size: 912 223 122 PpuMI rG'GwC_Cy Cuts at: 0 603 1014 1257 Size: 603 411 243 Fragments arranged by size: 603 411 243 SacII CC_GC'GG Cuts at: 0 1088 1257 Size: 1088 169 SexAI A'CCwGG_T Cuts at: 0 1178 1257 Size: 1178 79 SmaI CCC'GGG Cuts at: 0 544 1257 Size: 544 713 SmlI C'TyrA_G Cuts at: 0 595 1257 Size: 595 662 StyI C'CwwG_G Cuts at: 0 59 133 453 930 960 1257 Size: 59 74 320 477 30 297 Fragments arranged by size: 477 320 297 74 59 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 258 466 1257 Size: 258 208 791 Fragments arranged by size: 791 258 208 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 711 743 901 933 1257 Size: 711 32 158 32 324 Fragments arranged by size: 711 324 158 32 32 Tth111I GACn'n_nGTC Cuts at: 0 373 1257 Size: 373 884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 582 1257 Size: 582 675 XmaI C'CCGG_G Cuts at: 0 542 1257 Size: 542 715 Enzymes that do cut: Acc65I AflIII AhdI AloI AvaI AvrII BamHI BanI BbsI BciVI BfrBI BlpI Bme1580I BplI BpmI BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI ClaI EaeI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PfoI PpuMI SacII SexAI SmaI SmlI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BanII BbeI BbvCI BcgI BclI BglI BglII BmgBI BmrI BmtI Bpu10I BsaI BsaHI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XhoI XmnI ZraI