################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BanI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 640 GAATTT [GA]^AATT[CT] AhdI 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 369 GTGCAC G^TGCAC ApaLI 369 GTGCAC G^TGCAC ApoI 640 GAATTT [GA]^AATT[CT] AspEI 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 369 GTGCAC G[AT]GC[AT]^C AvaI 695 CCCGGG C^[CT]CG[GA]G BamHI 774 GGATCC G^GATCC BanII 275 GGGCTC G[GA]GC[CT]^C BbsI 801 GAAGAC GAAGAC(2/6) BbvCI 541 CCTCAGC CCTCAGC(-5/-2) BciVI 516 GTATCC GTATCC(6/5) BfaI 103 CTAG C^TAG BpmI 136 CTGGAG CTGGAG(16/14) BpuAI 801 GAAGAC GAAGAC(2/6) BsaWI 76 ACCGGA [AT]^CCGG[AT] BsiHKAI 369 GTGCAC G[AT]GC[AT]^C BsiYI 811 CCACTGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 811 CCACTGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 821 GAGAC GTCTC(1/5) BsoBI 695 CCCGGG C^[CT]CG[GA]G BsrDI 15 CATTGC GCAATG(2/0) BstYI 774 GGATCC [GA]^GATC[CT] DsaI 60 CCATGG C^C[GA][CT]GG Eam1105I 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 568 GATATC GAT^ATC EcoT14I 60 CCATGG C^C[AT][AT]GG HaeII 112 GGCGCT [GA]GCGC^[CT] HaeIII 855 GGCC GG^CC HindIII 878 AAGCTT A^AGCTT MaeI 103 CTAG C^TAG MaeII 458 ACGT A^CGT MflI 774 GGATCC [GA]^GATC[CT] NcoI 60 CCATGG C^CATGG NdeI 260 CATATG CA^TATG NspI 600 ACATGC [GA]CATG^[CT] PstI 739 CTGCAG CTGCA^G SfcI 739 CTGCAG C^T[GA][CT]AG SmaI 695 CCCGGG CCC^GGG StyI 60 CCATGG C^C[AT][AT]GG XhoII 774 GGATCC [GA]^GATC[CT] XmaCI 695 CCCGGG C^CCGGG XmaI 695 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 857 CCGC CCGC(-3/-1) 818 GCGG AvaII 301 GGTCC G^G[AT]CC 538 GGTCC BbvI 718 GCTGC GCAGC(8/12) 926 GCTGC BcnI 695 CCCGG CC^[GC]GG 696 CCGGG BmyI 275 GGGCTC G[AGT]GC[ACT]^C 369 GTGCAC Bsp1286I 275 GGGCTC G[AGT]GC[ACT]^C 369 GTGCAC BsrI 752 ACTGG ACTGG(1/-1) 813 ACTGG BstNI 392 CCAGG CC^[AT]GG 769 CCTGG CfoI 113 GCGC GCG^C 653 GCGC Cfr13I 301 GGTCC G^G[ACGT]CC 538 GGTCC EcoRII 392 CCAGG ^CC[AT]GG 769 CCTGG HapII 77 CCGG C^CGG 696 CCGG HhaI 113 GCGC GCG^C 653 GCGC HinP1I 113 GCGC G^CGC 653 GCGC HpaII 77 CCGG C^CGG 696 CCGG MaeIII 11 GTCAC ^GT[ACGT]AC 200 GTGAC MslI 61 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 622 CATTTCAATG MspI 77 CCGG C^CGG 696 CCGG MvaI 392 CCAGG CC^[AT]GG 769 CCTGG NciI 695 CCCGG CC^[GC]GG 696 CCGGG Sau96I 301 GGTCC G^G[ACGT]CC 538 GGTCC SfaNI 474 GCATC GCATC(5/9) 765 GCATC TseI 718 GCTGC G^C[AT]GC 926 GCTGC Tsp45I 11 GTCAC ^GT[GC]AC 200 GTGAC XcmI 353 CCACCTCTAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 512 CCAAGTATCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI AluI AlwI BsaJI BssKI BstF5I DdeI DpnII Fnu4HI FokI HinfI ItaI MboI MboII MnlI MseI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SmlI TfiI Tru9I Tsp509I TspRI