(Circular) (Six-base) MAPSORT of: pFN21AB7152.seq Check: 5269 from: 1 to: 5758 pFN21AB7152 5758 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 3821 5205 5473 1632 Size: 965 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 965 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2738 1166 Size: 1572 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 3041 3501 5073 3041 Size: 460 1572 3726 Fragments arranged by size: 3726 1572 460 ApaI G_GGCC'C Cuts at: 2404 2977 2404 Size: 573 5185 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 3067 3293 3445 5554 1493 Size: 1574 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2120 3083 1870 Size: 73 177 963 4545 Fragments arranged by size: 4545 963 177 73 BamHI G'GATC_C Cuts at: 2082 3088 3455 2082 Size: 1006 367 4385 Fragments arranged by size: 4385 1006 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2558 2795 3079 4534 618 Size: 325 217 627 771 237 284 1455 1842 Fragments arranged by size: 1842 1455 771 627 325 284 237 217 BanII G_rGCy'C Cuts at: 1391 2404 2673 2870 2977 3077 1391 Size: 1013 269 197 107 100 4072 Fragments arranged by size: 4072 1013 269 197 107 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2335 928 Size: 971 436 4351 Fragments arranged by size: 4351 971 436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2404 2798 2977 3819 4975 1790 Size: 614 394 179 842 1156 2573 Fragments arranged by size: 2573 1156 842 614 394 179 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2987 4418 1239 Size: 1748 1431 2579 Fragments arranged by size: 2579 1748 1431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2163 2616 4191 4589 4863 5010 5421 2163 Size: 453 1575 398 274 147 411 2500 Fragments arranged by size: 2500 1575 453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2016 2582 1186 Size: 830 566 4362 Fragments arranged by size: 4362 830 566 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2870 3819 3904 4975 2870 Size: 949 85 1071 3653 Fragments arranged by size: 3653 1071 949 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2404 2673 2798 2870 2977 3077 3819 Size: 399 614 269 125 72 107 100 742 Cuts at: 3819 3904 4975 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 399 269 125 107 100 85 72 BspEI T'CCGG_A Cuts at: 2163 5421 2163 Size: 3258 2500 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 2358 2673 3060 4253 4427 65 Size: 1183 892 218 315 387 1193 174 1396 Fragments arranged by size: 1396 1193 1183 892 387 315 218 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 2107 1184 Size: 923 4835 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2965 2263 Size: 702 5056 BstYI r'GATC_y Cuts at: 1972 2082 2685 3088 3455 3840 3857 5309 5395 Size: 110 603 403 367 385 17 1452 86 Cuts at: 5395 5407 5753 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 603 403 385 367 346 110 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2565 2565 Size: 5758 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 2262 513 Size: 552 939 252 6 4009 Fragments arranged by size: 4009 939 552 252 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2733 3274 4068 4088 2733 Size: 541 794 20 4403 Fragments arranged by size: 4403 794 541 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2314 2314 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2259 2427 Size: 54 1425 50 275 18 399 30 168 Cuts at: 2427 2583 3205 4094 5723 8 Size: 156 622 889 1629 43 Fragments arranged by size: 1629 1425 889 622 399 275 168 156 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3688 1353 Size: 2335 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2868 2868 Size: 5758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 2987 3821 4418 5205 5473 1239 Size: 393 965 390 834 597 787 268 1524 Fragments arranged by size: 1524 965 834 787 597 393 390 268 EcoNI CCTnn'n_nnAGG Cuts at: 2981 2981 Size: 5758 EcoO109I rG'GnC_Cy Cuts at: 2125 2728 2973 2974 2125 Size: 603 245 1 4909 Fragments arranged by size: 4909 603 245 1 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 2598 2630 4399 Size: 32 135 32 874 32 120 32 1769 Cuts at: 4399 4431 4473 4505 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1769 874 135 120 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3102 3348 669 Size: 988 281 919 245 246 3079 Fragments arranged by size: 3079 988 919 281 246 245 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 2857 3348 2857 Size: 491 5267 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2425 4872 5056 1305 Size: 1120 2447 184 2007 Fragments arranged by size: 2447 2007 1120 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2429 2585 10 Size: 54 1425 343 429 168 156 3183 Fragments arranged by size: 3183 1425 429 343 168 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2315 2435 2591 2738 3716 4075 Size: 648 1095 54 120 156 147 978 359 Cuts at: 4075 4234 518 Size: 159 2042 Fragments arranged by size: 2042 1095 978 648 359 159 156 147 120 54 MspA1I CmG'CkG Cuts at: 1606 2373 2889 3174 3851 4999 5244 1606 Size: 767 516 285 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 516 285 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2256 2262 513 Size: 552 939 252 6 4009 Fragments arranged by size: 4009 939 552 252 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2804 2836 3827 3859 5340 5372 2804 Size: 32 991 32 1481 32 3190 Fragments arranged by size: 3190 1481 991 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2728 2125 Size: 603 5155 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2400 2973 2400 Size: 573 5185 PstI C_TGCA'G Cuts at: 830 3037 3110 830 Size: 2207 73 3478 Fragments arranged by size: 3478 2207 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2889 2889 Size: 5758 SacI G_AGCT'C Cuts at: 2870 2870 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2499 3033 3106 4241 4922 5113 826 Size: 219 1454 534 73 1135 681 191 1471 Fragments arranged by size: 1471 1454 1135 681 534 219 191 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2262 2450 3197 513 Size: 552 939 252 6 188 747 3074 Fragments arranged by size: 3074 939 747 552 252 188 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 3785 3802 3955 4140 4585 325 Size: 1255 560 1645 17 153 185 445 1498 Fragments arranged by size: 1645 1498 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2531 2832 4004 Size: 274 80 33 51 1443 554 301 1172 Cuts at: 4004 5716 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 554 301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI