################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 289 GTCTAC GT^[AC][GT]AC AflII 249 CTTAAG C^TTAAG AflIII 25 ACGTGT A^C[GA][CT]GT BalI 30 TGGCCA TGG^CCA BamHI 498 GGATCC G^GATCC BanI 667 GGCACC G^G[CT][GA]CC BbrPI 805 CACGTG CAC^GTG BbsI 21 GAAGAC GAAGAC(2/6) BbvCI 769 GCTGAGG CCTCAGC(-5/-2) BfaI 349 CTAG C^TAG BfrI 249 CTTAAG C^TTAAG BpmI 362 CTCCAG CTGGAG(16/14) BpuAI 21 GAAGAC GAAGAC(2/6) BsaWI 210 ACCGGA [AT]^CCGG[AT] BsmAI 298 GTCTC GTCTC(1/5) BsmBI 297 CGTCTC CGTCTC(1/5) BsmFI 102 GTCCC GGGAC(10/14) BspMI 642 GCAGGT ACCTGC(4/8) BsrI 154 ACTGG ACTGG(1/-1) BstAPI 383 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 498 GGATCC [GA]^GATC[CT] EarI 160 CTCTTC CTCTTC(1/4) FauI 620 CCCGC CCCGC(4/6) HinfI 333 GACTC G^A[ACGT]TC Ksp632I 160 CTCTTC CTCTTC(1/4) MaeI 349 CTAG C^TAG MflI 498 GGATCC [GA]^GATC[CT] MluNI 30 TGGCCA TGG^CCA MscI 30 TGGCCA TGG^CCA MspA1I 637 CCGCTG C[AC]G^C[GT]G PflMI 33 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 333 GACTC GAGTC(4/5) PmaCI 805 CACGTG CAC^GTG PmlI 805 CACGTG CAC^GTG SacI 625 GAGCTC GAGCT^C SapI 159 GCTCTTC GCTCTTC(1/4) Tsp509I 285 AATT ^AATT TspRI 194 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 33 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 498 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 427 CGCG CG^CG 622 CGCG AspHI 625 GAGCTC G[AT]GC[AT]^C 802 GAGCAC AvaII 198 GGACC G^G[AT]CC 612 GGACC BanII 157 GGGCTC G[GA]GC[CT]^C 625 GAGCTC BcnI 69 CCCGG CC^[GC]GG 104 CCCGG BseRI 748 CTCCTC GAGGAG(10/8) 43 GAGGAG BsgI 390 CTGCAC GTGCAG(16/14) 679 CTGCAC BsiHKAI 625 GAGCTC G[AT]GC[AT]^C 802 GAGCAC BstEII 233 GGTCACC G^GT[ACGT]ACC 243 GGTAACC BstF5I 756 CATCC GGATG(2/0) 258 GGATG BstPI 233 GGTCACC G^GT[ACGT]ACC 243 GGTAACC BstUI 427 CGCG CG^CG 622 CGCG DdeI 486 CTCAG C^T[ACGT]AG 770 CTGAG EaeI 30 TGGCCA [CT]^GGCC[GA] 630 CGGCCA EcoO65I 233 GGTCACC G^GT[ACGT]ACC 243 GGTAACC FokI 756 CATCC GGATG(9/13) 258 GGATG HphI 147 GGTGA GGTGA(8/7) 235 TCACC MseI 250 TTAA T^TAA 542 TTAA MvnI 427 CGCG CG^CG 622 CGCG NciI 69 CCCGG CC^[GC]GG 104 CCCGG ScaI 404 AGTACT AGT^ACT 650 AGTACT SmlI 249 CTTAAG C^T[CT][GA]AG 859 CTCAAG StuI 276 AGGCCT AGG^CCT 489 AGGCCT Tru9I 250 TTAA T^TAA 542 TTAA Tsp45I 234 GTCAC ^GT[GC]AC 808 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BmyI BsaAI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI