Restriction map for
pFN21AE6233
(Circular) (Six-base) MAPSORT of: pFN21AE6233.seq Check: 8287 from: 1 to: 5578
pFN21AE6233 5578 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2295 2921 1937
Size: 358 626 4594
Fragments arranged by size:
4594 626 358
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2662 3641 5025 5293 1632
Size: 1030 979 1384 268 1917
Fragments arranged by size:
1917 1384 1030 979 268
AflII C'TTAA_G
Cuts at: 820 1017 2254 820
Size: 197 1237 4144
Fragments arranged by size:
4144 1237 197
AflIII A'CryG_T
Cuts at: 1203 2030 4477 5454 1203
Size: 827 2447 977 1327
Fragments arranged by size:
2447 1327 977 827
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2092 2319 2644 3321 4893 2092
Size: 227 325 677 1572 2777
Fragments arranged by size:
2777 1572 677 325 227
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 1870
Size: 73 960 4545
Fragments arranged by size:
4545 960 73
BamHI G'GATC_C
Cuts at: 2503 2908 3275 2503
Size: 405 367 4806
Fragments arranged by size:
4806 405 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2672 2899 4354 618
Size: 325 217 627 885 227 1455 1842
Fragments arranged by size:
1842 1455 885 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2166 2634 2897 1391
Size: 775 468 263 4072
Fragments arranged by size:
4072 775 468 263
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 928
Size: 971 134 4473
Fragments arranged by size:
4473 971 134
BbvCI CC'TCA_GC
Cuts at: 2775 2775
Size: 5578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4189 136
Size: 107 122 71 631 605 337 2180 1525
Fragments arranged by size:
2180 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 3639 4795 1790
Size: 1849 1156 2573
Fragments arranged by size:
2573 1849 1156
BmgBI CAC'GTC
Cuts at: 1318 2031 1318
Size: 713 4865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 3309 4267
Size: 416 53 83 399 24 752 1140 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1140 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 4238 1239
Size: 1113 1886 2579
Fragments arranged by size:
2579 1886 1113
Bpu10I CC'TnA_GC
Cuts at: 2491 2775 2491
Size: 284 5294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3703 4568 4866 2849
Size: 854 865 298 3561
Fragments arranged by size:
3561 865 854 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 2149 2812 3369 493
Size: 711 933 12 663 557 2702
Fragments arranged by size:
2702 933 711 663 557 12
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3385 3767 4402 275
Size: 53 83 186 936 1852 382 635 1451
Fragments arranged by size:
1852 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2215 4011 4409 4683 4830 5241 2215
Size: 1796 398 274 147 411 2552
Fragments arranged by size:
2552 1796 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2325 2355 2384 2414 1178
Size: 30 165 30 922 30 29 30 4342
Fragments arranged by size:
4342 922 165 30 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2744 1186
Size: 877 681 4020
Fragments arranged by size:
4020 877 681
BseYI C'CCAG_C
Cuts at: 2092 2576 2715 4781 5277 2092
Size: 484 139 2066 496 2393
Fragments arranged by size:
2393 2066 496 484 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2380 2669 1163
Size: 573 644 289 4072
Fragments arranged by size:
4072 644 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 2811 3639 3724 4795 2634
Size: 177 828 85 1071 3417
Fragments arranged by size:
3417 1071 828 177 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2308 2308
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2634 2811 2897 3639 3724 4795
Size: 399 376 468 177 86 742 85 1071
Cuts at: 4795 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 468 399 376 177 86
85
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 2933 844
Size: 1794 295 3489
Fragments arranged by size:
3489 1794 295
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4222 4409 1700
Size: 110 2412 187 2869
Fragments arranged by size:
2869 2412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2394 794
Size: 1600 3978
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 2238 2248 1184
Size: 1054 10 4514
Fragments arranged by size:
4514 1054 10
BstYI r'GATC_y
Cuts at: 1972 2503 2908 3275 3660 3677 5129 5215 5227
Size: 531 405 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 531 405 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4087
Fragments arranged by size:
4087 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2635 3025
Size: 54 1425 50 275 18 205 600 390
Cuts at: 3025 3914 5543 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 600 390 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3508 1353
Size: 818 1337 3423
Fragments arranged by size:
3423 1337 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2516 4165 4539 4685 387
Size: 1064 138 927 1649 374 146 1280
Fragments arranged by size:
1649 1280 1064 927 374 146 138
EcoICRI GAG'CTC
Cuts at: 2632 2632
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2662 3641 4238 5025 5293 1239
Size: 393 720 310 979 597 787 268 1524
Fragments arranged by size:
1524 979 787 720 597 393 310 268
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 4725 1678
Size: 3047 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4219 4251 4293 4325 1373
Size: 32 135 32 2647 32 42 32 2626
Fragments arranged by size:
2647 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2037 10
Size: 54 1425 343 205 3551
Fragments arranged by size:
3551 1425 343 205 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3536 3895 4054 518
Size: 648 2370 359 159 2042
Fragments arranged by size:
2370 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2644 2994 3671 4819 5064 1606
Size: 1038 350 677 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 350 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4087
Fragments arranged by size:
4087 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 1108
Size: 936 4642
PfoI T'CCnGG_A
Cuts at: 2418 2613 2418
Size: 195 5383
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PmlI CAC'GTG
Cuts at: 2812 2812
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2019 2484 2649 2930 830
Size: 1189 465 165 281 3478
Fragments arranged by size:
3478 1189 465 281 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
SacI G_AGCT'C
Cuts at: 2634 2634
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SapI GCTCTTCn'nnn_
Cuts at: 2171 2171
Size: 5578
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 2411 2657 3826 5538 2411
Size: 246 1169 1712 2451
Fragments arranged by size:
2451 1712 1169 246
SfcI C'TryA_G
Cuts at: 826 1045 2015 2480 2645 2725 2926 4061 4742
Size: 219 970 465 165 80 201 1135 681
Cuts at: 4742 4933 826
Size: 191 1471
Fragments arranged by size:
1471 1135 970 681 465 219 201 191
165 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2254 2864 3682 4583 4845 820
Size: 197 926 311 610 818 901 262 1553
Fragments arranged by size:
1553 926 901 818 610 311 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 2283 2496 1517
Size: 766 213 4599
Fragments arranged by size:
4599 766 213
StyI C'CwwG_G
Cuts at: 513 1065 2004 3017 513
Size: 552 939 1013 3074
Fragments arranged by size:
3074 1013 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2104 3605 3622 3775 3960 4405 325
Size: 1255 524 1501 17 153 185 445 1498
Fragments arranged by size:
1501 1498 1255 524 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2409 2655
Size: 274 80 33 51 1443 153 279 246
Cuts at: 2655 3824 5536 96
Size: 1169 1712 138
Fragments arranged by size:
1712 1443 1169 279 274 246 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2325 2357 5538 5570 2325
Size: 32 3181 32 2333
Fragments arranged by size:
3181 2333 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI