Restriction map for
pFN21AE6233
(Linear) (Six-base) MAPSORT of: KIEE6233.seq Check: 5106 from: 1 to: 873
KIEE6233.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 290 873
Size: 290 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 873
Size: 657 216
AflII C'TTAA_G
Cuts at: 0 249 873
Size: 249 624
AflIII A'CryG_T
Cuts at: 0 25 873
Size: 25 848
AlwNI CAG_nnn'CTG
Cuts at: 0 87 314 639 873
Size: 87 227 325 234
Fragments arranged by size:
325 234 227 87
BamHI G'GATC_C
Cuts at: 0 498 873
Size: 498 375
BanI G'GyrC_C
Cuts at: 0 667 873
Size: 667 206
BanII G_rGCy'C
Cuts at: 0 161 629 873
Size: 161 468 244
Fragments arranged by size:
468 244 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 873
Size: 28 845
BbvCI CC'TCA_GC
Cuts at: 0 770 873
Size: 770 103
BmgBI CAC'GTC
Cuts at: 0 26 873
Size: 26 847
BmrI ACTGGGnnnn_n'
Cuts at: 0 164 873
Size: 164 709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 873
Size: 347 526
Bpu10I CC'TnA_GC
Cuts at: 0 486 770 873
Size: 486 284 103
Fragments arranged by size:
486 284 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 873
Size: 844 29
BsaAI yAC'GTr
Cuts at: 0 132 144 807 873
Size: 132 12 663 66
Fragments arranged by size:
663 132 66 12
BsaWI w'CCGG_w
Cuts at: 0 210 873
Size: 210 663
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 320 350 379 409 873
Size: 320 30 29 30 464
Fragments arranged by size:
464 320 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 739 873
Size: 58 681 134
Fragments arranged by size:
681 134 58
BseYI C'CCAG_C
Cuts at: 0 87 571 710 873
Size: 87 484 139 163
Fragments arranged by size:
484 163 139 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 664 873
Size: 375 289 209
Fragments arranged by size:
375 289 209
BsiHKAI G_wGCw'C
Cuts at: 0 629 806 873
Size: 629 177 67
Fragments arranged by size:
629 177 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 303 873
Size: 303 570
Bsp1286I G_dGCh'C
Cuts at: 0 161 629 806 873
Size: 161 468 177 67
Fragments arranged by size:
468 177 161 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 873
Size: 633 240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 389 873
Size: 389 484
BstEII G'GTnAC_C
Cuts at: 0 233 243 873
Size: 233 10 630
Fragments arranged by size:
630 233 10
BstYI r'GATC_y
Cuts at: 0 498 873
Size: 498 375
EaeI y'GGCC_r
Cuts at: 0 30 630 873
Size: 30 600 243
Fragments arranged by size:
600 243 30
EarI CTCTTCn'nnn_
Cuts at: 0 166 873
Size: 166 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 511 873
Size: 511 362
EcoICRI GAG'CTC
Cuts at: 0 627 873
Size: 627 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 657 873
Size: 347 310 216
Fragments arranged by size:
347 310 216
MscI TGG'CCA
Cuts at: 0 32 873
Size: 32 841
MspA1I CmG'CkG
Cuts at: 0 639 873
Size: 639 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 873
Size: 39 834
PfoI T'CCnGG_A
Cuts at: 0 413 608 873
Size: 413 195 265
Fragments arranged by size:
413 265 195
PmlI CAC'GTG
Cuts at: 0 807 873
Size: 807 66
PstI C_TGCA'G
Cuts at: 0 14 479 644 873
Size: 14 465 165 229
Fragments arranged by size:
465 229 165 14
SacI G_AGCT'C
Cuts at: 0 629 873
Size: 629 244
SapI GCTCTTCn'nnn_
Cuts at: 0 166 873
Size: 166 707
ScaI AGT'ACT
Cuts at: 0 406 652 873
Size: 406 246 221
Fragments arranged by size:
406 246 221
SfcI C'TryA_G
Cuts at: 0 10 475 640 720 873
Size: 10 465 165 80 153
Fragments arranged by size:
465 165 153 80 10
SmlI C'TyrA_G
Cuts at: 0 249 859 873
Size: 249 610 14
Fragments arranged by size:
610 249 14
StuI AGG'CCT
Cuts at: 0 278 491 873
Size: 278 213 382
Fragments arranged by size:
382 278 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 99 873
Size: 99 774
TatI w'GTAC_w
Cuts at: 0 125 404 650 873
Size: 125 279 246 223
Fragments arranged by size:
279 246 223 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 320 352 873
Size: 320 32 521
Fragments arranged by size:
521 320 32
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI BamHI BanI BanII
BbsI BbvCI BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BstAPI BstEII BstYI EaeI EarI EciI EcoICRI
Eco57MI MscI MspA1I PflMI PfoI PmlI PstI SacI
SapI ScaI SfcI SmlI StuI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmtI BplI BsaI BsaBI
BsaHI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI